01541nam a2200217 a 450000100080000000500110000800800410001910000150006024501510007526000590022652008730028565000150115865000110117365000250118465000100120965300090121965300300122870000220125870000240128070000190130421444912023-09-06       2004    bl uuuu     u00u1 u    #d1 aNICKEL, O.  aProduction and Use of Polyclonal Antibodies to the Coat Protein of Apple stem grooving virus expressed in Escherichia coli.h[electronic resource]  aActa Horticulturae, v. 657, p. 35-40, ISHS 2004.c2004  aThe coat protein (cp) gene of Apple stem grooving Capillovirus was amplified by RT-PCR, cloned, sequenced and subcloned in the expression plasmid pMal-c2. The ASGV cp gene was expressed in E. coli as a fusion protein (fp) containing a fragment of E. coli maltose binding protein (MBP). Bacterial cells were disrupted by sonication and the ASGVcp/MBP-fp was purified by amylose resin affinity chromatography. Polyclonal antibodies from rabbits immunized with the fp, gave specific reactions to ASGV from several infected apple cultivars at dilutions of up to 1:2000 in indirect ELISA. The ASGVcp/MBP-fp reacted to antisera raised against it as well as to commercial antisera against ASGV virions in immunoblotting. These results are a fundamental contribution to the development of an efficient ASGVindexing of propagative and mother stock materials in Southern Brazil.  aAntibodies  aApples  aRecombinant antigens  aElisa  aASGV  aRecombinant viral antigen1 aFAJARDO, T. V. M.1 aTARGON, M. L. N. P.1 aMACHADO, M. A.