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Registro Completo |
Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
08/01/2021 |
Data da última atualização: |
22/03/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
PEREIRA, I. B.; CARVALHO, E. H. de S.; RODRIGUES, L. de B.; RODRIGUES, L. de B.; MAGALHAES, W. L. E.; LEME, D. M.; KRAWCZYK-SANTOS, A. P.; TAVEIRA, S. F.; OLIVEIRA, G. A. R. de. |
Afiliação: |
Iúri Barbosa Pereira, UFG; Endrew Henrique de Sousa Carvalho, UFG; Laís de Brito Rodrigues, UFG; Laís de Brito Rodrigues, Aalto University; WASHINGTON LUIZ ESTEVES MAGALHAES, CNPF; Daniela Morais Leme, UFPR; Anna Paula Krawczyk-Santos, UFG; Stephânia Fleury Taveira, UFG; Gisele Augusto Rodrigues de Oliveira, UFG. |
Título: |
Thymol-loaded biogenic silica nanoparticles on aquatic environment: the impact of particle aggregation on the ecotoxicity. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Environmental Toxicology and Chemistry, v. 40, n. 2, p. 333-341, 2021. |
DOI: |
https://doi.org/10.1002/etc.4938 |
Idioma: |
Inglês |
Conteúdo: |
Thymol (THY), a monoterpene phenol, is used as a natural biocide. To circumvent its chemical instability, we have proposed THY?loaded biogenic silica nanoparticle (BSiO2#THY); however, the toxicity of this system for aquatic organisms is unknown. Thus, this study aimed to evaluate the toxicogenetic effects induced by THY, BSiO2 nanoparticle (NP), and BSiO2#THY on Artemia salina and zebrafish (Danio rerio) early?life stages. We also investigated the impact of BSiO2 aggregation in the different exposure media (saline and freshwater). BSiO2#THY (LC50?48h = 1.06 mg/L) presented similar toxic potential as THY (LC50?48h = 1.03 mg/L) for A. salina, showing that BSiO2 had no influence on BSiO2#THY toxicity. As BSiO2 aggregated and sedimented faster in A. salina aqueous medium than in the other medium, this NP had lower interaction with this microcrustacean. Thus, BSiO2#THY toxicity for A. salina is probably due to the intrinsic toxicity of THY. For zebrafish early?life stages, BSiO2#THY (LC50?96h of 13.13 mg/L) was more toxic than free THY (LC50?96h of 25.60 mg/L); however, BSiO2 NP has no toxicity for zebrafish early?life stages. The lower aggregation of BSiO2 in the freshwater medium compared to the saline medium may have enhanced THY's availability for this aquatic organism. BSiO2#THY also significantly induced sublethal effects as THY, and both were genotoxic for zebrafish. In conclusion, although BSiO2#THY still needs improvements to ensure its safety for freshwater ecosystems, BSiO2 NP seems to be a safe nanocarrier for agriculture. MenosThymol (THY), a monoterpene phenol, is used as a natural biocide. To circumvent its chemical instability, we have proposed THY?loaded biogenic silica nanoparticle (BSiO2#THY); however, the toxicity of this system for aquatic organisms is unknown. Thus, this study aimed to evaluate the toxicogenetic effects induced by THY, BSiO2 nanoparticle (NP), and BSiO2#THY on Artemia salina and zebrafish (Danio rerio) early?life stages. We also investigated the impact of BSiO2 aggregation in the different exposure media (saline and freshwater). BSiO2#THY (LC50?48h = 1.06 mg/L) presented similar toxic potential as THY (LC50?48h = 1.03 mg/L) for A. salina, showing that BSiO2 had no influence on BSiO2#THY toxicity. As BSiO2 aggregated and sedimented faster in A. salina aqueous medium than in the other medium, this NP had lower interaction with this microcrustacean. Thus, BSiO2#THY toxicity for A. salina is probably due to the intrinsic toxicity of THY. For zebrafish early?life stages, BSiO2#THY (LC50?96h of 13.13 mg/L) was more toxic than free THY (LC50?96h of 25.60 mg/L); however, BSiO2 NP has no toxicity for zebrafish early?life stages. The lower aggregation of BSiO2 in the freshwater medium compared to the saline medium may have enhanced THY's availability for this aquatic organism. BSiO2#THY also significantly induced sublethal effects as THY, and both were genotoxic for zebrafish. In conclusion, although BSiO2#THY still needs improvements to ensure its safety for freshwater ecosystems,... Mostrar Tudo |
Palavras-Chave: |
Aggregation; Biocida; Biocide delivery systems; Comet assay; Nanopartícula; Teste cometa; Zebrafish. |
Thesagro: |
Artêmia Salina. |
Thesaurus Nal: |
Nanoparticles. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 02601naa a2200337 a 4500 001 2129128 005 2021-03-22 008 2021 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1002/etc.4938$2DOI 100 1 $aPEREIRA, I. B. 245 $aThymol-loaded biogenic silica nanoparticles on aquatic environment$bthe impact of particle aggregation on the ecotoxicity.$h[electronic resource] 260 $c2021 520 $aThymol (THY), a monoterpene phenol, is used as a natural biocide. To circumvent its chemical instability, we have proposed THY?loaded biogenic silica nanoparticle (BSiO2#THY); however, the toxicity of this system for aquatic organisms is unknown. Thus, this study aimed to evaluate the toxicogenetic effects induced by THY, BSiO2 nanoparticle (NP), and BSiO2#THY on Artemia salina and zebrafish (Danio rerio) early?life stages. We also investigated the impact of BSiO2 aggregation in the different exposure media (saline and freshwater). BSiO2#THY (LC50?48h = 1.06 mg/L) presented similar toxic potential as THY (LC50?48h = 1.03 mg/L) for A. salina, showing that BSiO2 had no influence on BSiO2#THY toxicity. As BSiO2 aggregated and sedimented faster in A. salina aqueous medium than in the other medium, this NP had lower interaction with this microcrustacean. Thus, BSiO2#THY toxicity for A. salina is probably due to the intrinsic toxicity of THY. For zebrafish early?life stages, BSiO2#THY (LC50?96h of 13.13 mg/L) was more toxic than free THY (LC50?96h of 25.60 mg/L); however, BSiO2 NP has no toxicity for zebrafish early?life stages. The lower aggregation of BSiO2 in the freshwater medium compared to the saline medium may have enhanced THY's availability for this aquatic organism. BSiO2#THY also significantly induced sublethal effects as THY, and both were genotoxic for zebrafish. In conclusion, although BSiO2#THY still needs improvements to ensure its safety for freshwater ecosystems, BSiO2 NP seems to be a safe nanocarrier for agriculture. 650 $aNanoparticles 650 $aArtêmia Salina 653 $aAggregation 653 $aBiocida 653 $aBiocide delivery systems 653 $aComet assay 653 $aNanopartícula 653 $aTeste cometa 653 $aZebrafish 700 1 $aCARVALHO, E. H. de S. 700 1 $aRODRIGUES, L. de B. 700 1 $aRODRIGUES, L. de B. 700 1 $aMAGALHAES, W. L. E. 700 1 $aLEME, D. M. 700 1 $aKRAWCZYK-SANTOS, A. P. 700 1 $aTAVEIRA, S. F. 700 1 $aOLIVEIRA, G. A. R. de 773 $tEnvironmental Toxicology and Chemistry$gv. 40, n. 2, p. 333-341, 2021.
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Embrapa Florestas (CNPF) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
30/10/2017 |
Data da última atualização: |
31/10/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
BEZERRA JUNIOR, R. Q.; ELOY, A. M. X.; FURTADO, J. R.; PINHEIRO, R. R.; ANDRIOLI, A.; MORENO, F. B.; LOBO, M. D. P.; MONTEIRO-MOREIRA, A. C. O.; MOREIRA, R. de A.; PINTO, T. M. F.; TEIXEIRA, M. F. da S. |
Afiliação: |
Rosivaldo Quirino Bezerra Júnior, Laboratory of Virology (LABOVIR), Ceará State University (UECE) - Fortaleza, Ceará, Brazil; ANGELA MARIA XAVIER ELOY, CNPC; JOAO RICARDO FURTADO, CNPC; RAYMUNDO RIZALDO PINHEIRO, CNPC; ALICE ANDRIOLI, CNPC; Frederico Bruno Moreno, Center of Experimental Biology (Nubex), University of Fortaleza (UNIFOR), Fortaleza, Ceará, Brazil; Marina Duarte Pinto Lobo, Center of Experimental Biology (Nubex), University of Fortaleza (UNIFOR), Fortaleza, Ceará, Brazil; Ana Cristina O. Monteiro-Moreira, Center of Experimental Biology (Nubex), University of Fortaleza (UNIFOR), Fortaleza, Ceará, Brazil; Renato de Azevedo Moreira; Tatiana Maria Farias Pinto, Molecular Biology Laboratory, Vale do Acaraú State University (UVA) - Sobral, Ceará, Brazil; Maria Fátima da Silva Teixeira, Laboratory of Virology (LABOVIR), Ceará State University (UECE) - Fortaleza, Ceará, Brazil. |
Título: |
A panel of protein candidates for comprehensive study of Caprine Arthritis Encephalitis (CAE) infection. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Tropical Animal Health and Production, v. 50, n. 1, n. 7, p. 43-48, Oct. 2018. |
DOI: |
10.1007/s11250-017-1398-1 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: The caprine arthrite encephalite (CAE) is a disease that affects especially dairy goat. The virus shows compartmentalization features, that allows it to hide at certain times during the course of the disease, making it difficult to control. The present study was conducted to identify the major seminal plasma protein profile of goats infected by CAE and its associations with seroconversion using Western blotting. Two groups containing five males each, were used in this experiment. The first group was composed by seropositive animals and the control by seronegative confirmed by Western blotting and PCR. The semen was collected through artificial vagina and after that, two-dimensional electrophoresis and MALDI-TOF MS were used. Seventy-five spots were identified in the goat seminal plasma gels, equivalent to 13 different proteins with more expression. The similar proteins found in both groups and related to reproduction were spermadhesin Z13-like, bodhesin and bodhesin-2, Lipocalin, protein PDC-109-like, and albumin. In infected goats, proteases such as arisulfatase A have been identified, whose function probably is related to metabolism control of sulfatides, involved to virus control. The other ones were bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase, cathepsin F isoform X1, disintegrin and metalloproteinase domain-containing protein 2-like isoform X1, clusterin, carbonic anhydrase 2, electron transfer flavoprotein subunit beta, and epididymal secretory glutathione peroxidase. The results of this study show the reaction of the innate immune system against chronic infection of goats by CAE. MenosAbstract: The caprine arthrite encephalite (CAE) is a disease that affects especially dairy goat. The virus shows compartmentalization features, that allows it to hide at certain times during the course of the disease, making it difficult to control. The present study was conducted to identify the major seminal plasma protein profile of goats infected by CAE and its associations with seroconversion using Western blotting. Two groups containing five males each, were used in this experiment. The first group was composed by seropositive animals and the control by seronegative confirmed by Western blotting and PCR. The semen was collected through artificial vagina and after that, two-dimensional electrophoresis and MALDI-TOF MS were used. Seventy-five spots were identified in the goat seminal plasma gels, equivalent to 13 different proteins with more expression. The similar proteins found in both groups and related to reproduction were spermadhesin Z13-like, bodhesin and bodhesin-2, Lipocalin, protein PDC-109-like, and albumin. In infected goats, proteases such as arisulfatase A have been identified, whose function probably is related to metabolism control of sulfatides, involved to virus control. The other ones were bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase, cathepsin F isoform X1, disintegrin and metalloproteinase domain-containing protein 2-like isoform X1, clusterin, carbonic anhydrase 2, electron transfer flavoprotein subunit beta, and epididymal secr... Mostrar Tudo |
Palavras-Chave: |
Artrite Encefalite Caprina; Arylsulfatase A; Proteomic; Viroses; Virus disease. |
Thesagro: |
Caprino; Doença animal; Genética Molecular; Infecção; Marcador genético; Sêmen; Vírus. |
Thesaurus NAL: |
Biomarkers; Caprine arthritis encephalitis virus; Dairy goats; Genetic markers; Infectious diseases; Lentivirus; Proteomics; Viral proteins. |
Categoria do assunto: |
H Saúde e Patologia |
Marc: |
LEADER 03050naa a2200493 a 4500 001 2078384 005 2023-10-31 008 2018 bl uuuu u00u1 u #d 024 7 $a10.1007/s11250-017-1398-1$2DOI 100 1 $aBEZERRA JUNIOR, R. Q. 245 $aA panel of protein candidates for comprehensive study of Caprine Arthritis Encephalitis (CAE) infection. 260 $c2018 520 $aAbstract: The caprine arthrite encephalite (CAE) is a disease that affects especially dairy goat. The virus shows compartmentalization features, that allows it to hide at certain times during the course of the disease, making it difficult to control. The present study was conducted to identify the major seminal plasma protein profile of goats infected by CAE and its associations with seroconversion using Western blotting. Two groups containing five males each, were used in this experiment. The first group was composed by seropositive animals and the control by seronegative confirmed by Western blotting and PCR. The semen was collected through artificial vagina and after that, two-dimensional electrophoresis and MALDI-TOF MS were used. Seventy-five spots were identified in the goat seminal plasma gels, equivalent to 13 different proteins with more expression. The similar proteins found in both groups and related to reproduction were spermadhesin Z13-like, bodhesin and bodhesin-2, Lipocalin, protein PDC-109-like, and albumin. In infected goats, proteases such as arisulfatase A have been identified, whose function probably is related to metabolism control of sulfatides, involved to virus control. The other ones were bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase, cathepsin F isoform X1, disintegrin and metalloproteinase domain-containing protein 2-like isoform X1, clusterin, carbonic anhydrase 2, electron transfer flavoprotein subunit beta, and epididymal secretory glutathione peroxidase. The results of this study show the reaction of the innate immune system against chronic infection of goats by CAE. 650 $aBiomarkers 650 $aCaprine arthritis encephalitis virus 650 $aDairy goats 650 $aGenetic markers 650 $aInfectious diseases 650 $aLentivirus 650 $aProteomics 650 $aViral proteins 650 $aCaprino 650 $aDoença animal 650 $aGenética Molecular 650 $aInfecção 650 $aMarcador genético 650 $aSêmen 650 $aVírus 653 $aArtrite Encefalite Caprina 653 $aArylsulfatase A 653 $aProteomic 653 $aViroses 653 $aVirus disease 700 1 $aELOY, A. M. X. 700 1 $aFURTADO, J. R. 700 1 $aPINHEIRO, R. R. 700 1 $aANDRIOLI, A. 700 1 $aMORENO, F. B. 700 1 $aLOBO, M. D. P. 700 1 $aMONTEIRO-MOREIRA, A. C. O. 700 1 $aMOREIRA, R. de A. 700 1 $aPINTO, T. M. F. 700 1 $aTEIXEIRA, M. F. da S. 773 $tTropical Animal Health and Production$gv. 50, n. 1, n. 7, p. 43-48, Oct. 2018.
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