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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
08/10/2014 |
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Data da última atualização: |
08/10/2014 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUZA, A. L.; ANGELO, P. C. da S.; NOGUEIRA, P. P. O.; GONÇALVES, J. F. C.; FRANCO, A. M.; ASTOLFI-FILHO, S.; LÓPEZ-LOZANO, J. L.; ANDRADE, E. V. |
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Afiliação: |
A. L. Souza, UFAM; PAULA CRISTINA DA SILVA ANGELO, CPAA; P. P. O. Nogueira, FIOCRUZ; J. F. C. Gonçalves, INPA; A. M. Franco, INPA; S. Astolfi-Filho, UFAM; J. L. López-Lozano, Fundação de Medicina Tropical do Amazonas; E. V. Andrade, UFAM. |
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Título: |
Method for obtaining high-resolution proteomic analysis from pericarps of guarana. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 13, n. 3, p. 8014-8024, 2014. |
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DOI: |
http://dx.doi.org/10.4238/2014.September.29.14 |
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Idioma: |
Inglês |
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Conteúdo: |
Guarana has great agricultural potential and is largely used therapeutically and in the production of non-alcoholic energy drinks. Genomic and proteomic studies are crucial to identify proteins that play central roles in the maintenance and viability of fruits, as well as to identify proteins related to the main metabolic pathways. However, the success of any protein analysis starts with the protein extract preparation, which needs to offer an extract that is free of contaminants. This study aimed to evaluate different extraction methods to obtain high-quantity and high-quality extracts that are compatible with analysis by 2-dimensional electrophoresis and tandem mass spectrometry protein identification. Three different methods were tested: trichloroacetic acid (TCA)/acetone, sodium dodecyl sulfate (SDS)/phenol, and polyvinylpolypyrrolidone (PVPP)/SDS/phenol. |
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Palavras-Chave: |
Plant proteomics; Two-dimensional electrophoresis. |
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Thesagro: |
Guaraná; Paullinia Cupana. |
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Thesaurus Nal: |
polyphenols. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/109684/1/gmr4011.pdf
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Marc: |
LEADER 01739naa a2200277 a 4500
001 1996859
005 2014-10-08
008 2014 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.4238/2014.September.29.14$2DOI
100 1 $aSOUZA, A. L.
245 $aMethod for obtaining high-resolution proteomic analysis from pericarps of guarana.$h[electronic resource]
260 $c2014
520 $aGuarana has great agricultural potential and is largely used therapeutically and in the production of non-alcoholic energy drinks. Genomic and proteomic studies are crucial to identify proteins that play central roles in the maintenance and viability of fruits, as well as to identify proteins related to the main metabolic pathways. However, the success of any protein analysis starts with the protein extract preparation, which needs to offer an extract that is free of contaminants. This study aimed to evaluate different extraction methods to obtain high-quantity and high-quality extracts that are compatible with analysis by 2-dimensional electrophoresis and tandem mass spectrometry protein identification. Three different methods were tested: trichloroacetic acid (TCA)/acetone, sodium dodecyl sulfate (SDS)/phenol, and polyvinylpolypyrrolidone (PVPP)/SDS/phenol.
650 $apolyphenols
650 $aGuaraná
650 $aPaullinia Cupana
653 $aPlant proteomics
653 $aTwo-dimensional electrophoresis
700 1 $aANGELO, P. C. da S.
700 1 $aNOGUEIRA, P. P. O.
700 1 $aGONÇALVES, J. F. C.
700 1 $aFRANCO, A. M.
700 1 $aASTOLFI-FILHO, S.
700 1 $aLÓPEZ-LOZANO, J. L.
700 1 $aANDRADE, E. V.
773 $tGenetics and Molecular Research$gv. 13, n. 3, p. 8014-8024, 2014.
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Registro original: |
Embrapa Amazônia Ocidental (CPAA) |
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Voltar
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pantanal. Para informações adicionais entre em contato com cpap.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
07/10/2020 |
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Data da última atualização: |
07/10/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
SILVA, W. A. L da; POEHLAND, R.; OLIVEIRA, C. C. de; FERREIRA, M. G. C. R.; ALMEIDA, R. G. de; CÁCERES, M. B. S.; MACEDO, G. G.; COSTA e SILVA, E. V. da; ALVES, F. V.; NOGUEIRA, E.; MELO-STERZA, F. de A. |
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Afiliação: |
WILIAN APARECIDO LEITE DA SILVA, State University of Mato Grosso do Sul,Aquidauana, Mato Grosso do Sul; RALF POEHLAND, Institute of ReproductiveBiology, Dummerstorf, Germany; CAROLINE CARVALHO DE OLIVEIRA, Federal University of Mato Grosso do Sul, CampoGrande, Mato Grosso do Sul; MARIANE GABRIELA CESAR RIBEIRO FERREIRA, Federal University of Mato Grosso do Sul, CampoGrande, Mato Grosso do Sul; RICARDO GARCIA DE ALMEIDA, Federal University of Mato Grosso do Sul, CampoGrande; MIRELA BROCHADO SOUZA CÁCERES, University of Londrina, Londrina; GUSTAVO GUERINO MACEDO, Federal University of Mato Grosso do Sul, Campo Grande; ELIANE VIANNA DA COSTA E SILVA, Federal University of Mato Grosso do Sul, Campo Grande; FABIANA VILLA ALVES, CNPGC; ERIKLIS NOGUEIRA, CPAP; FABIANA DE ANDRADE MELO-STERZA, University of Mato Grosso do Sul,Aquidauana, Mato Grosso do Sul. |
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Título: |
Shading effect on physiological parameters and in vitro embryoproduction of tropical adapted Nellore heifers in integratedcrop-livestock-forest systems. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Tropical Animal Health and Production, v. 52, n. 5, p. 2273-2281, 2020. |
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DOI: |
https://doi.org/10.1007/s11250-020-02244-3 |
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Idioma: |
Português |
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Conteúdo: |
The aim of this study was to evaluate the impact of increased shadow supply in integrated crop-livestock-forest systems onin vitro embryonic development and physiological parameters related to stress response in Nellore heifers (Bos indicus). For thestudy, animals (n= 16) were randomly divided into two groups and kept in areas with different afforestation systems, theintegrated crop-livestock-forest (ICLF) and the integrated crop-livestock (ICL) system. The microclimate of the ICLF systemprovided better comfort conditions than ICL. No differences of respiratory rate, rectal temperature, cortisol, T3, T4, oocytequality, and cleavage rate between the systems were verified. A higher blastocyst rate was observed in the ICLF (p<0.05).Theresults demonstrate that Nellore heifers managed in ICLF during summer in Midwest of Brazil showed higher production ofin vitro embryos, without typical changes in its physiological parameters. The results observed in the present study indicate thatzebu females are able to respond satisfactorily to the intense heat conditions; however, we believe that the long period to whichthese animals are exposed to these conditions interferes in the oocyte competence and embryo development. |
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Palavras-Chave: |
ILPF. |
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Thesagro: |
Novilho; Pecuária; Stress. |
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Thesaurus NAL: |
Heifers; Stress management. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02254naa a2200325 a 4500
001 2125344
005 2020-10-07
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s11250-020-02244-3$2DOI
100 1 $aSILVA, W. A. L da
245 $aShading effect on physiological parameters and in vitro embryoproduction of tropical adapted Nellore heifers in integratedcrop-livestock-forest systems.$h[electronic resource]
260 $c2020
520 $aThe aim of this study was to evaluate the impact of increased shadow supply in integrated crop-livestock-forest systems onin vitro embryonic development and physiological parameters related to stress response in Nellore heifers (Bos indicus). For thestudy, animals (n= 16) were randomly divided into two groups and kept in areas with different afforestation systems, theintegrated crop-livestock-forest (ICLF) and the integrated crop-livestock (ICL) system. The microclimate of the ICLF systemprovided better comfort conditions than ICL. No differences of respiratory rate, rectal temperature, cortisol, T3, T4, oocytequality, and cleavage rate between the systems were verified. A higher blastocyst rate was observed in the ICLF (p<0.05).Theresults demonstrate that Nellore heifers managed in ICLF during summer in Midwest of Brazil showed higher production ofin vitro embryos, without typical changes in its physiological parameters. The results observed in the present study indicate thatzebu females are able to respond satisfactorily to the intense heat conditions; however, we believe that the long period to whichthese animals are exposed to these conditions interferes in the oocyte competence and embryo development.
650 $aHeifers
650 $aStress management
650 $aNovilho
650 $aPecuária
650 $aStress
653 $aILPF
700 1 $aPOEHLAND, R.
700 1 $aOLIVEIRA, C. C. de
700 1 $aFERREIRA, M. G. C. R.
700 1 $aALMEIDA, R. G. de
700 1 $aCÁCERES, M. B. S.
700 1 $aMACEDO, G. G.
700 1 $aCOSTA e SILVA, E. V. da
700 1 $aALVES, F. V.
700 1 $aNOGUEIRA, E.
700 1 $aMELO-STERZA, F. de A.
773 $tTropical Animal Health and Production$gv. 52, n. 5, p. 2273-2281, 2020.
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