01739naa a2200277 a 450000100080000000500110000800800410001902400560006010000170011624501100013326000090024352008760025265000160112865000130114465000210115765300210117865300360119970000240123570000230125970000250128270000180130770000220132570000250134770000190137277300700139119968592014-10-08       2014    bl uuuu     u00u1 u    #d7 ahttp://dx.doi.org/10.4238/2014.September.29.142DOI1 aSOUZA, A. L.  aMethod for obtaining high-resolution proteomic analysis from pericarps of guarana.h[electronic resource]  c2014  aGuarana has great agricultural potential and is largely used therapeutically and in the production of non-alcoholic energy drinks. Genomic and proteomic studies are crucial to identify proteins that play central roles in the maintenance and viability of fruits, as well as to identify proteins related to the main metabolic pathways. However, the success of any protein analysis starts with the protein extract preparation, which needs to offer an extract that is free of contaminants. This study aimed to evaluate different extraction methods to obtain high-quantity and high-quality extracts that are compatible with analysis by 2-dimensional electrophoresis and tandem mass spectrometry protein identification. Three different methods were tested: trichloroacetic acid (TCA)/acetone, sodium dodecyl sulfate (SDS)/phenol, and polyvinylpolypyrrolidone (PVPP)/SDS/phenol.  apolyphenols  aGuaraná  aPaullinia Cupana  aPlant proteomics  aTwo-dimensional electrophoresis1 aANGELO, P. C. da S.1 aNOGUEIRA, P. P. O.1 aGONÇALVES, J. F. C.1 aFRANCO, A. M.1 aASTOLFI-FILHO, S.1 aLÓPEZ-LOZANO, J. L.1 aANDRADE, E. V.  tGenetics and Molecular Researchgv. 13, n. 3, p. 8014-8024, 2014.