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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
30/12/2019 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PILLAI, V. V.; SIQUEIRA, L. G. B.; DAS, M.; KEI, T. G.; TU, L. N.; HERREN, A. W.; PHINNEY, B. S.; CHEONG, S. H.; HANSEN, P. J.; SELVARAJ, V. |
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Afiliação: |
VIJU VIJAYAN PILLAI, Cornell University, Ithaca, NY; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; MOUBANI DAS, Cornell University, Ithaca, NY; TIFFANY G. KEI, Cornell University, Ithaca, NY; LAN N. TU, Cornell University, Ithaca, NY; ANTHONY W. HERREN, University of California; BRETT S. PHINNEY, University of California; SOON HON CHEONG, Cornell University, Ithaca, NY; PETER J. HANSEN, University of Florida, Gainesville, FL; VIMAL SELVARAJ, Cornell University, Ithaca, NY. |
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Título: |
Physiological profile of undifferentiated bovine blastocyst-derived trophoblasts. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Biology Open, v. 8, n. 5, article bio037937, 2019. |
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DOI: |
https://doi.org/10.1242/bio.037937 |
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Idioma: |
Inglês |
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Conteúdo: |
Trophectoderm of blastocysts mediate early events in fetal-maternal communication, enabling implantation and establishment of a functional placenta. Inadequate or impaired developmental events linked to trophoblasts directly impact early embryo survival and successful implantation during a crucial period that corresponds with high incidence of pregnancy losses in dairy cows. As yet, the molecular basis of bovine trophectoderm development and signaling towards initiation of implantation remains poorly understood. In this study, we developed methods for culturing undifferentiated bovine blastocyst-derived trophoblasts and used both transcriptomics and proteomics in early colonies to categorize and elucidate their functional characteristics. A total of 9270 transcripts and 1418 proteins were identified and analyzed based on absolute abundance. We profiled an extensive list of growth factors, cytokines and other relevant factors that can effectively influence paracrine communication in the uterine microenvironment. Functional categorization and analysis revealed novel information on structural organization, extracellular matrix composition, cell junction and adhesion components, transcription networks, and metabolic preferences. Our data showcase the fundamental physiology of bovine trophectoderm and indicate hallmarks of the self-renewing undifferentiated state akin to trophoblast stem cells described in other species. Functional features uncovered are essential for understanding early events in bovine pregnancy towards initiation of implantation. MenosTrophectoderm of blastocysts mediate early events in fetal-maternal communication, enabling implantation and establishment of a functional placenta. Inadequate or impaired developmental events linked to trophoblasts directly impact early embryo survival and successful implantation during a crucial period that corresponds with high incidence of pregnancy losses in dairy cows. As yet, the molecular basis of bovine trophectoderm development and signaling towards initiation of implantation remains poorly understood. In this study, we developed methods for culturing undifferentiated bovine blastocyst-derived trophoblasts and used both transcriptomics and proteomics in early colonies to categorize and elucidate their functional characteristics. A total of 9270 transcripts and 1418 proteins were identified and analyzed based on absolute abundance. We profiled an extensive list of growth factors, cytokines and other relevant factors that can effectively influence paracrine communication in the uterine microenvironment. Functional categorization and analysis revealed novel information on structural organization, extracellular matrix composition, cell junction and adhesion components, transcription networks, and metabolic preferences. Our data showcase the fundamental physiology of bovine trophectoderm and indicate hallmarks of the self-renewing undifferentiated state akin to trophoblast stem cells described in other species. Functional features uncovered are essential for understandi... Mostrar Tudo |
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Thesagro: |
Placenta. |
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Thesaurus Nal: |
Blastocyst; Embryo implantation; Pregnancy; Stem cells; Trophoblast. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/207915/1/BiolOpen-LGustavoSiq-Physiological-bio037937.full.pdf
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Marc: |
LEADER 02435naa a2200313 a 4500
001 2117829
005 2024-02-06
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1242/bio.037937$2DOI
100 1 $aPILLAI, V. V.
245 $aPhysiological profile of undifferentiated bovine blastocyst-derived trophoblasts.$h[electronic resource]
260 $c2019
520 $aTrophectoderm of blastocysts mediate early events in fetal-maternal communication, enabling implantation and establishment of a functional placenta. Inadequate or impaired developmental events linked to trophoblasts directly impact early embryo survival and successful implantation during a crucial period that corresponds with high incidence of pregnancy losses in dairy cows. As yet, the molecular basis of bovine trophectoderm development and signaling towards initiation of implantation remains poorly understood. In this study, we developed methods for culturing undifferentiated bovine blastocyst-derived trophoblasts and used both transcriptomics and proteomics in early colonies to categorize and elucidate their functional characteristics. A total of 9270 transcripts and 1418 proteins were identified and analyzed based on absolute abundance. We profiled an extensive list of growth factors, cytokines and other relevant factors that can effectively influence paracrine communication in the uterine microenvironment. Functional categorization and analysis revealed novel information on structural organization, extracellular matrix composition, cell junction and adhesion components, transcription networks, and metabolic preferences. Our data showcase the fundamental physiology of bovine trophectoderm and indicate hallmarks of the self-renewing undifferentiated state akin to trophoblast stem cells described in other species. Functional features uncovered are essential for understanding early events in bovine pregnancy towards initiation of implantation.
650 $aBlastocyst
650 $aEmbryo implantation
650 $aPregnancy
650 $aStem cells
650 $aTrophoblast
650 $aPlacenta
700 1 $aSIQUEIRA, L. G. B.
700 1 $aDAS, M.
700 1 $aKEI, T. G.
700 1 $aTU, L. N.
700 1 $aHERREN, A. W.
700 1 $aPHINNEY, B. S.
700 1 $aCHEONG, S. H.
700 1 $aHANSEN, P. J.
700 1 $aSELVARAJ, V.
773 $tBiology Open$gv. 8, n. 5, article bio037937, 2019.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
28/11/2006 |
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Data da última atualização: |
28/11/2006 |
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Autoria: |
CARVALHO, J. E. B. de. |
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Título: |
Manejo de superfície do solo com coberturas vegetais na cultura do mamão em Tabuleiros Costeiros. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
Cruz das Almas: Embrapa Mandioca e Fruticultura Tropical, 2006. |
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Idioma: |
Português |
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Notas: |
1 folder |
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Conteúdo: |
O mamoeiro necessita de uma boa aeração e de espaços vazios no solo para bom desenvolvimento do sistema radicular e conseqüentemente aumento da produção. A cultura do mamão é uma das mais exigentes quanto ao consumo de água, sofrendo severa concorrência de água pela competição das plantas infestantes em determinadas épocas do ano. Nos Tabuleiros Costeiros, as características do solo evidencia ainda mais essa competição sendo que o manejo empregado atualmente pelos produtores tem contribuido para o aumento da compactação do solo, com redução do ar e macroporos, refletindo negativamente na produtividade e longevidade do mamoeiro. |
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Palavras-Chave: |
Cobertura vegetais; Tratamentos. |
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Thesagro: |
Cobertura Vegetal; Controle Químico; Mamão; Manejo; Solo. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01251nam a2200205 a 4500
001 1653559
005 2006-11-28
008 2006 bl uuuu u0uu1 u #d
100 1 $aCARVALHO, J. E. B. de
245 $aManejo de superfície do solo com coberturas vegetais na cultura do mamão em Tabuleiros Costeiros.
260 $aCruz das Almas: Embrapa Mandioca e Fruticultura Tropical$c2006
500 $a1 folder
520 $aO mamoeiro necessita de uma boa aeração e de espaços vazios no solo para bom desenvolvimento do sistema radicular e conseqüentemente aumento da produção. A cultura do mamão é uma das mais exigentes quanto ao consumo de água, sofrendo severa concorrência de água pela competição das plantas infestantes em determinadas épocas do ano. Nos Tabuleiros Costeiros, as características do solo evidencia ainda mais essa competição sendo que o manejo empregado atualmente pelos produtores tem contribuido para o aumento da compactação do solo, com redução do ar e macroporos, refletindo negativamente na produtividade e longevidade do mamoeiro.
650 $aCobertura Vegetal
650 $aControle Químico
650 $aMamão
650 $aManejo
650 $aSolo
653 $aCobertura vegetais
653 $aTratamentos
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