02435naa a2200313 a 450000100080000000500110000800800410001902400440006010000180010424501090012226000090023152015750024065000150181565000240183065000140185465000150186865000160188365000130189970000230191270000120193570000150194770000140196270000180197670000190199470000180201370000180203170000170204977300550206621178292024-02-06       2019    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1242/bio.0379372DOI1 aPILLAI, V. V.  aPhysiological profile of undifferentiated bovine blastocyst-derived trophoblasts.h[electronic resource]  c2019  aTrophectoderm of blastocysts mediate early events in fetal-maternal communication, enabling implantation and establishment of a functional placenta. Inadequate or impaired developmental events linked to trophoblasts directly impact early embryo survival and successful implantation during a crucial period that corresponds with high incidence of pregnancy losses in dairy cows. As yet, the molecular basis of bovine trophectoderm development and signaling towards initiation of implantation remains poorly understood. In this study, we developed methods for culturing undifferentiated bovine blastocyst-derived trophoblasts and used both transcriptomics and proteomics in early colonies to categorize and elucidate their functional characteristics. A total of 9270 transcripts and 1418 proteins were identified and analyzed based on absolute abundance. We profiled an extensive list of growth factors, cytokines and other relevant factors that can effectively influence paracrine communication in the uterine microenvironment. Functional categorization and analysis revealed novel information on structural organization, extracellular matrix composition, cell junction and adhesion components, transcription networks, and metabolic preferences. Our data showcase the fundamental physiology of bovine trophectoderm and indicate hallmarks of the self-renewing undifferentiated state akin to trophoblast stem cells described in other species. Functional features uncovered are essential for understanding early events in bovine pregnancy towards initiation of implantation.  aBlastocyst  aEmbryo implantation  aPregnancy  aStem cells  aTrophoblast  aPlacenta1 aSIQUEIRA, L. G. B.1 aDAS, M.1 aKEI, T. G.1 aTU, L. N.1 aHERREN, A. W.1 aPHINNEY, B. S.1 aCHEONG, S. H.1 aHANSEN, P. J.1 aSELVARAJ, V.  tBiology Opengv. 8, n. 5, article bio037937, 2019.