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Registro Completo |
Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Agroenergia; Embrapa Meio-Norte. |
Data corrente: |
21/05/2019 |
Data da última atualização: |
18/11/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
CASARI, R. A. C. N.; PAIVA, D. S.; SILVA, V. N. B.; FERREIRA, T. M. M.; SOUZA JUNIOR, M. T.; OLIVEIRA, N. G.; KOBAYASHI, A. K.; MOLINARI, H. B. C.; SANTOS, T. T.; GOMIDE, R. L.; MAGALHÃES, P. C.; SOUSA, C. A. F. de. |
Afiliação: |
Raphael Augusto das Chagas Noqueli Casari, Bolsista da Embrapa Agroenergia; Dayane S. Paiva, Bolsista da Embrapa Agroenergia; Vivianny N. B. Silva, Bolsista da Embrapa Agroenergia; Thalita M. M. Ferreira, Bolsista da Embrapa Agroenergia; MANOEL TEIXEIRA SOUZA JUNIOR, CNPAE; Nelson G. Oliveira, Bolsista da Embrapa Agroenergia; ADILSON KENJI KOBAYASHI, CNPAE; HUGO BRUNO CORREA MOLINARI, CNPAE. |
Título: |
Using thermography to confirm genotypic variation for drought response in maize. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
International Journal of Molecular Sciences v. 20, n. 9, 2273, May-1, 2019. |
DOI: |
10.3390/ijms20092273 |
Idioma: |
Inglês Português |
Conteúdo: |
The feasibility of thermography as a technique for plant screening aiming at drought-tolerance has been proven by its relationship with gas exchange, biomass, and yield. In this study, unlike most of the previous, thermography was applied for phenotyping contrasting maize genotypes whose classification for drought tolerance had already been established in the field. Our objective was to determine whether thermography-based classification would discriminate the maize genotypes in a similar way as the field selection in which just grain yield was taken into account as a criterion. We evaluated gas exchange, daily water consumption, leaf relative water content, aboveground biomass, and grain yield. Indeed, the screening of maize genotypes based on canopy temperature showed similar results to traditional methods. Nevertheless, canopy temperature only partially reflected gas exchange rates and daily water consumption in plants under drought. Part of the explanation may lie in the changes that drought had caused in plant leaves and canopy structure, altering absorption and dissipation of energy, photosynthesis, transpiration, and partitioning rates. Accordingly, although there was a negative relationship between grain yield and plant canopy temperature, it does not necessarily mean that plants whose canopies were maintained cooler under drought achieved the highest yield. |
Palavras-Chave: |
Canopy temperature; Déficit hídrico; Fenotipagem de plantas; Plant phenotyping; Termografia; Thermal image; Tolerância à seca; Water deficit. |
Thesagro: |
Milho; Zea Mays. |
Thesaurus Nal: |
Abiotic stress; Drought tolerance; Thermography. |
Categoria do assunto: |
-- X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/198719/1/SEG-05.12.12.001.00.05-HUGO-3-ijms-20-02273-v2.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/197552/1/Artigo-termografia.pdf
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Marc: |
LEADER 02607naa a2200421 a 4500 001 2109974 005 2019-11-18 008 2019 bl uuuu u00u1 u #d 024 7 $a10.3390/ijms20092273$2DOI 100 1 $aCASARI, R. A. C. N. 245 $aUsing thermography to confirm genotypic variation for drought response in maize.$h[electronic resource] 260 $c2019 520 $aThe feasibility of thermography as a technique for plant screening aiming at drought-tolerance has been proven by its relationship with gas exchange, biomass, and yield. In this study, unlike most of the previous, thermography was applied for phenotyping contrasting maize genotypes whose classification for drought tolerance had already been established in the field. Our objective was to determine whether thermography-based classification would discriminate the maize genotypes in a similar way as the field selection in which just grain yield was taken into account as a criterion. We evaluated gas exchange, daily water consumption, leaf relative water content, aboveground biomass, and grain yield. Indeed, the screening of maize genotypes based on canopy temperature showed similar results to traditional methods. Nevertheless, canopy temperature only partially reflected gas exchange rates and daily water consumption in plants under drought. Part of the explanation may lie in the changes that drought had caused in plant leaves and canopy structure, altering absorption and dissipation of energy, photosynthesis, transpiration, and partitioning rates. Accordingly, although there was a negative relationship between grain yield and plant canopy temperature, it does not necessarily mean that plants whose canopies were maintained cooler under drought achieved the highest yield. 650 $aAbiotic stress 650 $aDrought tolerance 650 $aThermography 650 $aMilho 650 $aZea Mays 653 $aCanopy temperature 653 $aDéficit hídrico 653 $aFenotipagem de plantas 653 $aPlant phenotyping 653 $aTermografia 653 $aThermal image 653 $aTolerância à seca 653 $aWater deficit 700 1 $aPAIVA, D. S. 700 1 $aSILVA, V. N. B. 700 1 $aFERREIRA, T. M. M. 700 1 $aSOUZA JUNIOR, M. T. 700 1 $aOLIVEIRA, N. G. 700 1 $aKOBAYASHI, A. K. 700 1 $aMOLINARI, H. B. C. 700 1 $aSANTOS, T. T. 700 1 $aGOMIDE, R. L. 700 1 $aMAGALHÃES, P. C. 700 1 $aSOUSA, C. A. F. de 773 $tInternational Journal of Molecular Sciences$gv. 20, n. 9, 2273, May-1, 2019.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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Registro Completo
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
07/05/2020 |
Data da última atualização: |
15/12/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
SOUSA, A. M. P.; SALLES, H. O.; OLIVEIRA, H. D. de; SOUZA, B. B. P. de; CARDOZO FILHO, J. de L.; SIFUENTES, D. N.; PRATES, M. V.; BLOCH JUNIOR, C.; BEMQUERER, M. P.; VASCONCELOS, A. S. do E. |
Afiliação: |
ANA MARJORY PAIVA SOUSA, Rede Nordeste de Biotecnologia-RENORBIO, Universidade Estadual do Ceará (UECe) - Fortaleza, CE, Brazil; HEVILA OLIVEIRA SALLES, CNPC; HERMOGENES DAVID DE OLIVEIRA, Universidade Federal do Ceará (UFC) - Fortaleza, CE, Brazil; BEATRIZ BLENDA PINHEIRO DE SOUZA; JOSE DE LIMA CARDOZO FILHO; DANIEL NOGOCEKE SIFUENTES, Cenargen; MAURA VIANNA PRATES, Cenargen; CARLOS BLOCH JUNIOR, Cenargen; MARCELO PORTO BEMQUERER, Cenargen; ANTONIO SILVIO DO EGITO VASCONCELOS, CNPC. |
Título: |
Mo-HLPs: new flocculating agents identified from Moringa oleifera seeds belong to the hevein-like peptide family. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Journal of Proteomics, v. 217, article 103692, Apr. 2020. |
DOI: |
https://doi.org/10.1016/j.jprot.2020.103692 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: Cationic peptides found in Moringa oleifera seeds belong to different protein families and are described as the main flocculating agents of the species. In this study we report the identification and isolation of four new flocculant peptides, called Mo-HLPs 1?4, belonging to the family of hevein-like peptides, previously only known for their members' antimicrobial activity. Purification of the peptides followed two sequential membrane ultrafiltration steps and separation by reverse-phase liquid chromatography. Proteomic analyses showed that Mo-HLPs are extremely basic (pI >10) cysteine-rich molecules with molecular masses between 4.5 and 4.8 kDa and with a highly conserved chitin-binding domain. Searches in BLAST revealed high similarity of Mo-HLPs with hevein and other hevein-like peptides and 90% identity with morintides, which are members of the 8C-hevein-like subfamily found in M. oleifera leaves. Mo-HLPs microflocculation assays showed distinct coagulation/flocculation efficiencies, promoting turbidity reduction levels between 67 and 89% in synthetic turbid water. Activity variations were attributed to the substitution of some amino acids among the isoforms, which may have altered the final net charge of the molecules. The identification of Mo-HLPs represents the discovery of a new group of cationic peptides involved in the flocculation properties of M. oleifera seeds. |
Palavras-Chave: |
Cationic peptides; Flocculant activity; Hevein-like. |
Thesagro: |
Moringa Oleifera. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02333naa a2200289 a 4500 001 2122135 005 2020-12-15 008 2020 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.jprot.2020.103692$2DOI 100 1 $aSOUSA, A. M. P. 245 $aMo-HLPs$bnew flocculating agents identified from Moringa oleifera seeds belong to the hevein-like peptide family.$h[electronic resource] 260 $c2020 520 $aAbstract: Cationic peptides found in Moringa oleifera seeds belong to different protein families and are described as the main flocculating agents of the species. In this study we report the identification and isolation of four new flocculant peptides, called Mo-HLPs 1?4, belonging to the family of hevein-like peptides, previously only known for their members' antimicrobial activity. Purification of the peptides followed two sequential membrane ultrafiltration steps and separation by reverse-phase liquid chromatography. Proteomic analyses showed that Mo-HLPs are extremely basic (pI >10) cysteine-rich molecules with molecular masses between 4.5 and 4.8 kDa and with a highly conserved chitin-binding domain. Searches in BLAST revealed high similarity of Mo-HLPs with hevein and other hevein-like peptides and 90% identity with morintides, which are members of the 8C-hevein-like subfamily found in M. oleifera leaves. Mo-HLPs microflocculation assays showed distinct coagulation/flocculation efficiencies, promoting turbidity reduction levels between 67 and 89% in synthetic turbid water. Activity variations were attributed to the substitution of some amino acids among the isoforms, which may have altered the final net charge of the molecules. The identification of Mo-HLPs represents the discovery of a new group of cationic peptides involved in the flocculation properties of M. oleifera seeds. 650 $aMoringa Oleifera 653 $aCationic peptides 653 $aFlocculant activity 653 $aHevein-like 700 1 $aSALLES, H. O. 700 1 $aOLIVEIRA, H. D. de 700 1 $aSOUZA, B. B. P. de 700 1 $aCARDOZO FILHO, J. de L. 700 1 $aSIFUENTES, D. N. 700 1 $aPRATES, M. V. 700 1 $aBLOCH JUNIOR, C. 700 1 $aBEMQUERER, M. P. 700 1 $aVASCONCELOS, A. S. do E. 773 $tJournal of Proteomics$gv. 217, article 103692, Apr. 2020.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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