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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
07/04/2004 |
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Data da última atualização: |
11/09/2013 |
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Autoria: |
LEMOS, N. G.; YAMAGUCHI-SHINOZAKI, K.; NAKASHIMA, K.; MOLINA, J. C.; STOLF, R.; MORALES, A. M. R.; LUGLE, S. M.; SILVEIRA, C. A. de; MARIN, S. R. R.; BINNECK, E.; FARIAS, J. R. B.; NEUMAIER, N.; NEPOMUCENO, A. L. |
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Título: |
Introduction of genes that confer drought stress tolerance in soybean [Glycine max (L.) Merril] by biobalistic. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. |
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Páginas: |
p. 256. |
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Série: |
(Embrapa Soja. Documentos, 228). |
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Idioma: |
Inglês |
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Notas: |
Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi. |
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Conteúdo: |
The soybean crop moves the Brazilian economy from the primary sector to the industry, generating many direct and indirect jobs. However, yearly billions of dolars are lost due abiotic and biotic stresses occurring during the crop cycle. Abiotic stresses, precisely drought, is one of the main causes of yield losses. Aiming to minimize this damage, scientists are using biotechnology methods, introducing genes in plants to improve tolerance to drought stress. The plant drought response is regulated by a complex of genes generating a variety of tolerance mecanisms. The complexity of such responses make difficult of using classic breeding methods in the development tolerant varieties. However, genetic engineering of key genes in key metabolic pathways has succeded in incresing drought tolerance in many crops. Our objective was to introduce in soybean the expression cassets pRD29:DREB1 and pE35S:P5CS-129A, using an Embrapa's patent method of Biobalistics that confer dehydration tolerance in plants. DREB1A (Dehidration Responsive Biding Element Protein) is a transcription factor responsible by the activation of genes involved in the drought, freezing and saline stress response; P5CS-129A is also a key enzime in the pathway for proline production. Proline increasing in the cytosol has been related to the cell turgor preservation and protection of cell structures during dehydration by scavenging oxidative radicals. The transformed plants have been generated and tested by PCR. More than 2590 embryos were transformed until now. Nineteen plants were PCR positives for the AHAS gene and none PCR positive plants were detected until now for the p35S:P5CS-129A construct. Number of insertion copies and expression levels will be tested in the positive plants. Physiological characterization in drought conditions will be carried on in promissing events. MenosThe soybean crop moves the Brazilian economy from the primary sector to the industry, generating many direct and indirect jobs. However, yearly billions of dolars are lost due abiotic and biotic stresses occurring during the crop cycle. Abiotic stresses, precisely drought, is one of the main causes of yield losses. Aiming to minimize this damage, scientists are using biotechnology methods, introducing genes in plants to improve tolerance to drought stress. The plant drought response is regulated by a complex of genes generating a variety of tolerance mecanisms. The complexity of such responses make difficult of using classic breeding methods in the development tolerant varieties. However, genetic engineering of key genes in key metabolic pathways has succeded in incresing drought tolerance in many crops. Our objective was to introduce in soybean the expression cassets pRD29:DREB1 and pE35S:P5CS-129A, using an Embrapa's patent method of Biobalistics that confer dehydration tolerance in plants. DREB1A (Dehidration Responsive Biding Element Protein) is a transcription factor responsible by the activation of genes involved in the drought, freezing and saline stress response; P5CS-129A is also a key enzime in the pathway for proline production. Proline increasing in the cytosol has been related to the cell turgor preservation and protection of cell structures during dehydration by scavenging oxidative radicals. The transformed plants have been generated and tested by PCR. More th... Mostrar Tudo |
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Thesagro: |
Soja. |
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Categoria do assunto: |
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Marc: |
LEADER 03105naa a2200313 a 4500
001 1466831
005 2013-09-11
008 2004 bl uuuu u00u1 u #d
100 1 $aLEMOS, N. G.
245 $aIntroduction of genes that confer drought stress tolerance in soybean [Glycine max (L.) Merril] by biobalistic.
260 $c2004
300 $ap. 256.
490 $a(Embrapa Soja. Documentos, 228).
500 $aEditado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.
520 $aThe soybean crop moves the Brazilian economy from the primary sector to the industry, generating many direct and indirect jobs. However, yearly billions of dolars are lost due abiotic and biotic stresses occurring during the crop cycle. Abiotic stresses, precisely drought, is one of the main causes of yield losses. Aiming to minimize this damage, scientists are using biotechnology methods, introducing genes in plants to improve tolerance to drought stress. The plant drought response is regulated by a complex of genes generating a variety of tolerance mecanisms. The complexity of such responses make difficult of using classic breeding methods in the development tolerant varieties. However, genetic engineering of key genes in key metabolic pathways has succeded in incresing drought tolerance in many crops. Our objective was to introduce in soybean the expression cassets pRD29:DREB1 and pE35S:P5CS-129A, using an Embrapa's patent method of Biobalistics that confer dehydration tolerance in plants. DREB1A (Dehidration Responsive Biding Element Protein) is a transcription factor responsible by the activation of genes involved in the drought, freezing and saline stress response; P5CS-129A is also a key enzime in the pathway for proline production. Proline increasing in the cytosol has been related to the cell turgor preservation and protection of cell structures during dehydration by scavenging oxidative radicals. The transformed plants have been generated and tested by PCR. More than 2590 embryos were transformed until now. Nineteen plants were PCR positives for the AHAS gene and none PCR positive plants were detected until now for the p35S:P5CS-129A construct. Number of insertion copies and expression levels will be tested in the positive plants. Physiological characterization in drought conditions will be carried on in promissing events.
650 $aSoja
700 1 $aYAMAGUCHI-SHINOZAKI, K.
700 1 $aNAKASHIMA, K.
700 1 $aMOLINA, J. C.
700 1 $aSTOLF, R.
700 1 $aMORALES, A. M. R.
700 1 $aLUGLE, S. M.
700 1 $aSILVEIRA, C. A. de
700 1 $aMARIN, S. R. R.
700 1 $aBINNECK, E.
700 1 $aFARIAS, J. R. B.
700 1 $aNEUMAIER, N.
700 1 $aNEPOMUCENO, A. L.
773 $tIn: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004.
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Registro original: |
Embrapa Soja (CNPSO) |
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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
05/09/2018 |
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Data da última atualização: |
23/09/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
BRAIR, V. L.; SCHMIDT, A. P. P.; FIGUEIRA, L. M.; VERGANI, G. B.; DIÓGENES, Y. P.; RANGEL, P. S. C.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da. |
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Afiliação: |
Universidade do Grande Rio (UNIGRANRIO) - Duque de Caxias, RJ, Brazil; Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; Universidade Federal de Lavras (UFLA) - Lavras, MG, Brasil; Universidade Estadual Paulista (UNESP) - Jaboticabal, SP, Brazil; Universidade Estadual do Ceará (UECE) - Fortaleza, CE, Brazil; Universidade do Grande Rio (UNIGRANRIO) - Duque de Caxias, RJ, Brazil; Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; Jeferson Ferreira da Fonseca, CNPC. |
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Título: |
Vaginal cytology as a tool to predict the time of ovulation in goats and sheep. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Animal Reprodroduction, v. 15, n. 3, p. 492, Jul./Sept. 2018. Abstracts A202. Edição dos Proceedings of the 32nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Florianópolis, SC, Brazil, August 16th to 18th, 2018. |
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Idioma: |
Inglês |
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Conteúdo: |
The detection of ovulation is of great importance for the use of reproductive biotechnologies in small ruminants. The ovulation is efficiently determined by ultrasound (US), equipment that is not always available and of relatively high cost. Therefore, the aim of this study was to identify the efficacy of vaginal cytology as a tool to determine the ovulation time in these species. The study was carried out during the non-breeding season, in Coronel Pacheco, Minas Gerais (21°35'S and 43°15'W). In sheep, the standard chosen was anucleated, but it had a high CV (23.7%), which led to non-high accuracy (66.7%). Therefore, PPV and NPV were 26% and 88%, respectively; the specificity was 45% and 64% at 24 h and 12 h before ovulation respectively; and finally, the sensitivity at the moment of ovulation was 55%. It can be concluded that the vaginal cytology may be an efficient tool to determine the moment of ovulation in goats, however it is less accurate in sheep. |
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Palavras-Chave: |
Citologia vaginal; Ovarian echography. |
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Thesagro: |
Caprino; Citologia; Ovino; Ovulação; Reprodução Animal. |
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Thesaurus NAL: |
Animal reproduction; Ewes; Goats; Ovulation; Sheep; Ultrasonography; Vaginal mucosa. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/182469/1/cnpc2018-Vaginal.pdf
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Marc: |
LEADER 02139nam a2200361 a 4500
001 2095108
005 2019-09-23
008 2018 bl uuuu u00u1 u #d
100 1 $aBRAIR, V. L.
245 $aVaginal cytology as a tool to predict the time of ovulation in goats and sheep.$h[electronic resource]
260 $aAnimal Reprodroduction, v. 15, n. 3, p. 492, Jul./Sept. 2018. Abstracts A202. Edição dos Proceedings of the 32nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Florianópolis, SC, Brazil, August 16th to 18th$c2018
520 $aThe detection of ovulation is of great importance for the use of reproductive biotechnologies in small ruminants. The ovulation is efficiently determined by ultrasound (US), equipment that is not always available and of relatively high cost. Therefore, the aim of this study was to identify the efficacy of vaginal cytology as a tool to determine the ovulation time in these species. The study was carried out during the non-breeding season, in Coronel Pacheco, Minas Gerais (21°35'S and 43°15'W). In sheep, the standard chosen was anucleated, but it had a high CV (23.7%), which led to non-high accuracy (66.7%). Therefore, PPV and NPV were 26% and 88%, respectively; the specificity was 45% and 64% at 24 h and 12 h before ovulation respectively; and finally, the sensitivity at the moment of ovulation was 55%. It can be concluded that the vaginal cytology may be an efficient tool to determine the moment of ovulation in goats, however it is less accurate in sheep.
650 $aAnimal reproduction
650 $aEwes
650 $aGoats
650 $aOvulation
650 $aSheep
650 $aUltrasonography
650 $aVaginal mucosa
650 $aCaprino
650 $aCitologia
650 $aOvino
650 $aOvulação
650 $aReprodução Animal
653 $aCitologia vaginal
653 $aOvarian echography
700 1 $aSCHMIDT, A. P. P.
700 1 $aFIGUEIRA, L. M.
700 1 $aVERGANI, G. B.
700 1 $aDIÓGENES, Y. P.
700 1 $aRANGEL, P. S. C.
700 1 $aSOUZA-FABJAN, J. M. G.
700 1 $aFONSECA, J. F. da
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Embrapa Caprinos e Ovinos (CNPC) |
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