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Registro Completo |
Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
04/01/2016 |
Data da última atualização: |
10/03/2016 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
LOPES, L. D.; LIMA, A. O. S.; TAKETANI, R. G.; DARIAS, P.; SILVA, L. R. F.; ROMAGNOLI, E. M.; LOUVANDINI, H.; ABDALLA, A. L.; MENDES, R. |
Afiliação: |
LUCAS DANTAS LOPES; ANDRE OLIVEIRA DE SOUZA LIMA, UNIVALI; RODRIGO GOUVEA TAKETANI; PHILLIP DARIAS, UNIVALI; LILIA RAQUEL FE DA SILVA, UFPI; EMILIANA MANESCO ROMAGNOLI; HELDER LOUVANDINI, CENA-USP; ADIBE LUIZ ABDALLA, CENA-USP; RODRIGO MENDES, CNPMA. |
Título: |
Exploring the sheep rumen microbiome for carbohydrate-active enzymes. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Antonie van Leeuwenhoek, Amsterdam, v. 108, n. 1, p. 15-30, 2015. |
Idioma: |
Inglês |
Conteúdo: |
Abstract: The rumen is a complex ecosystem enriched for microorganisms able to degrade biomass during the animal?s digestion process. The recovery of new enzymes from naturally evolved biomass-degrading microbial communities is a promising strategy to overcome the inefficient enzymatic plant destruction in industrial production of biofuels. In this context, this study aimed to describe the bacterial composition and functions in the sheep rumen microbiome, focusing on carbohydrate-active enzymes (CAE). Here, we used phylogenetic profiling analysis (inventory of 16S rRNA genes) combined with metagenomics to access the rumen microbiome of four sheep and explore its potential to identify fibrolytic enzymes. The bacterial community was dominated by Bacteroidetes and Firmicutes, followed by Proteobacteria. As observed for other ruminants, Prevotella was the dominant genus in the microbiome, comprising more than 30 % of the total bacterial community. Multivariate analysis of the phylogenetic profiling data and chemical parameters showed a positive correlation between the abundance of Prevotellaceae (Bacteroidetes phylum) and organic matter degradability. A negative correlation was observed between Succinivibrionaceae (Proteobacteria phylum) and methane production. An average of 2 % of the shotgun metagenomic reads was assigned to putative CAE when considering nine protein databases. In addition, assembled contigs allowed recognition of 67 putative partial CAE (NCBI-Refseq) representing 12 glycosyl hydrolase families (Pfam database). Overall, we identified a total of 28 lignocellulases, 22 amylases and 9 other putative CAE, showing the sheep rumen microbiome as a promising source of new fibrolytic enzymes MenosAbstract: The rumen is a complex ecosystem enriched for microorganisms able to degrade biomass during the animal?s digestion process. The recovery of new enzymes from naturally evolved biomass-degrading microbial communities is a promising strategy to overcome the inefficient enzymatic plant destruction in industrial production of biofuels. In this context, this study aimed to describe the bacterial composition and functions in the sheep rumen microbiome, focusing on carbohydrate-active enzymes (CAE). Here, we used phylogenetic profiling analysis (inventory of 16S rRNA genes) combined with metagenomics to access the rumen microbiome of four sheep and explore its potential to identify fibrolytic enzymes. The bacterial community was dominated by Bacteroidetes and Firmicutes, followed by Proteobacteria. As observed for other ruminants, Prevotella was the dominant genus in the microbiome, comprising more than 30 % of the total bacterial community. Multivariate analysis of the phylogenetic profiling data and chemical parameters showed a positive correlation between the abundance of Prevotellaceae (Bacteroidetes phylum) and organic matter degradability. A negative correlation was observed between Succinivibrionaceae (Proteobacteria phylum) and methane production. An average of 2 % of the shotgun metagenomic reads was assigned to putative CAE when considering nine protein databases. In addition, assembled contigs allowed recognition of 67 putative partial CAE (NCBI-Refseq) represen... Mostrar Tudo |
Palavras-Chave: |
Ion torrent (PGM). |
Thesagro: |
Amilase; Bactéria; Enzima; Ovelha; Rúmen. |
Thesaurus Nal: |
Amylases; Bacterial communities; Lignocellulose; Metagenomics; Phylogeny. |
Categoria do assunto: |
S Ciências Biológicas |
Marc: |
LEADER 02652naa a2200349 a 4500 001 2032734 005 2016-03-10 008 2015 bl uuuu u00u1 u #d 100 1 $aLOPES, L. D. 245 $aExploring the sheep rumen microbiome for carbohydrate-active enzymes.$h[electronic resource] 260 $c2015 520 $aAbstract: The rumen is a complex ecosystem enriched for microorganisms able to degrade biomass during the animal?s digestion process. The recovery of new enzymes from naturally evolved biomass-degrading microbial communities is a promising strategy to overcome the inefficient enzymatic plant destruction in industrial production of biofuels. In this context, this study aimed to describe the bacterial composition and functions in the sheep rumen microbiome, focusing on carbohydrate-active enzymes (CAE). Here, we used phylogenetic profiling analysis (inventory of 16S rRNA genes) combined with metagenomics to access the rumen microbiome of four sheep and explore its potential to identify fibrolytic enzymes. The bacterial community was dominated by Bacteroidetes and Firmicutes, followed by Proteobacteria. As observed for other ruminants, Prevotella was the dominant genus in the microbiome, comprising more than 30 % of the total bacterial community. Multivariate analysis of the phylogenetic profiling data and chemical parameters showed a positive correlation between the abundance of Prevotellaceae (Bacteroidetes phylum) and organic matter degradability. A negative correlation was observed between Succinivibrionaceae (Proteobacteria phylum) and methane production. An average of 2 % of the shotgun metagenomic reads was assigned to putative CAE when considering nine protein databases. In addition, assembled contigs allowed recognition of 67 putative partial CAE (NCBI-Refseq) representing 12 glycosyl hydrolase families (Pfam database). Overall, we identified a total of 28 lignocellulases, 22 amylases and 9 other putative CAE, showing the sheep rumen microbiome as a promising source of new fibrolytic enzymes 650 $aAmylases 650 $aBacterial communities 650 $aLignocellulose 650 $aMetagenomics 650 $aPhylogeny 650 $aAmilase 650 $aBactéria 650 $aEnzima 650 $aOvelha 650 $aRúmen 653 $aIon torrent (PGM) 700 1 $aLIMA, A. O. S. 700 1 $aTAKETANI, R. G. 700 1 $aDARIAS, P. 700 1 $aSILVA, L. R. F. 700 1 $aROMAGNOLI, E. M. 700 1 $aLOUVANDINI, H. 700 1 $aABDALLA, A. L. 700 1 $aMENDES, R. 773 $tAntonie van Leeuwenhoek, Amsterdam$gv. 108, n. 1, p. 15-30, 2015.
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Embrapa Meio Ambiente (CNPMA) |
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Registros recuperados : 63 | |
41. | | SOARES JUNIOR, F. L.; DIAS, A. C. F.; FASANELLA, C. C.; TAKETANI, R. G.; LIMA, A. O. S.; MELO, I. S. de; ANDREOTE, F. D. Endo- and exoglucanase activities in bacteria from mangrove sediment. Brazilian Journal of Microbiology, Piracicaba, v. 44, n. 3, p. 969-976, 2013.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Meio Ambiente. |
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42. | | MOITINHO, M. A.; CHIARAMONTE, J. B.; SOUZA, D. T.; SOLANO, J. H.; BONONI, L.; MELO, I. S. de; TAKETANI, R. G. Intraspecific variation on epiphytic bacterial community from Laguncularia racemosa phylloplane. Brazilian Journal of Microbiology, v. 50, n. 4, p. 1041-1050, 2019.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Meio Ambiente. |
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43. | | TAKETANI, N.; TAKETANI, R. G.; MELO, I. S. de; RIZZO, A. C. L.; SORIANO, A. U.; LEITE, S. G. F.; CUNHA, C. D. Influence of nickel in petroleum biodegradation: a metagenomics approach. In: INTERNATIONAL SYMPOSIUM ON MICROBIAL ECOLOGY, 15., 2014, Seoul. Proceedings... Wageningen: The International Society for Microbial Ecology (ISME), 2014. p. 606-607.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Meio Ambiente. |
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44. | | DIAS, A. C. F.; TAKETANI, R. G.; ANDREOTE, F. D.; LUVIZOTTO, D. M.; SILVA, J. L. da; NASCIMENTO, R. dos S.; MELO, I. S. de. Interspecific variation of the bacterial community structure in the Phyllosphere of the three major plant components of mangrove forests. Brazilian Journal of Microbiology, São Paulo, v. 43, n. 2, p. 653-660, 2012.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Meio Ambiente. |
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45. | | FERREIRA, C.; TAKETANI, R. G.; SILVA, J. L. da; GAVA, C. A. T.; LOPES, L. D.; MELO, I. S. de; MENDES, R. Descoberta da dinâmica do microbioma da rizosfera de mandacaru na caatinga. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 27., 2013, Natal. Anais... Natal: Sociedade Brasileira de Microbiologia, 2013. Resumo 1640-1Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Meio Ambiente. |
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46. | | ROMAGNOLI, E. M.; NATEL, A. S.; FAGUNDES, G. G.; DURRER, A.; TAKETANI, R. G.; LOUVANDINI, H; ABDALLA, A. L.; MENDES, R. Deep into to the bacterial communities living in sheep rumen. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 27., 2013, Natal. Anais... Natal: Sociedade Brasileira de Microbiologia, 2013. Resumo 1377-1.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Meio Ambiente. |
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47. | | LOPES, L. D.; SILVA, L. R. F.; ROMAGNOLI, E. M.; FERREIRA, C; TAKETANI, R. G.; LOUVANDINI, H; LIMA, A. O. S.; ABDALLA, A. L.; MENDES, R. Sheep rumen microbiome sequencing using Ion Torrent (PGM) platform. In: SYMPOSIUM ON BACTERIAL GENETICS AND ECOLOGY, 12., 2013, Ljubljana (Slovenia). Networking and plasticity of microbial communities: the secret to success. Ljubljana: University of Ljubljana. 2013. p. 68, ref. P31.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Meio Ambiente. |
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48. | | LOPES, L. D.; LIMA, A. O. S.; TAKETANI, R. G.; DARIAS, P.; SILVA, L. R. F.; ROMAGNOLI, E. M.; LOUVANDINI, H.; ABDALLA, A. L.; MENDES, R. Exploring the sheep rumen microbiome for carbohydrate-active enzymes. Antonie van Leeuwenhoek, Amsterdam, v. 108, n. 1, p. 15-30, 2015.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Meio Ambiente. |
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49. | | LOPES, L. D.; LIMA, A. O. S.; SILVA, L. R. F.; ROMAGNOLI, E. M.; TAKETANI, R. G.; FERREIRA, C; ABDALLA, A. L.; MENDES, R. Identificação de enzimas lignocelulolíticas no microbioma do rúmen de ovinos usando metagenômica shotgun. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 27., 2013, Natal. Anais... Natal: Sociedade Brasileira de Microbiologia, 2013. Resumo 901-1Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Meio Ambiente. |
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50. | | VASCONCELLOS, R. L. de F.; ROMAGNOLI, E. M.; TAKETANI, R. G.; SANTOS, S. N.; ZUCCHI, T. D.; MELO, I. S. de. Impact of inoculation with Pseudomonas aestus CMAA 1215T on the non-target resident bacterial community in a saline rhizosphere soil. Current Microbiology, v. 78, n. 1, p. 218-228, 2020.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Meio Ambiente. |
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51. | | MARCON, J.; TAKETANI, R. G.; DINI-ANDREOTE, F.; MAZZERO, G. I.; SOARES JUNIOR, F. L.; MELO, I. S. de; AZEVEDO, J. L.; ANDREOTE, F. D. Draft genome sequence of Bacillus thuringiensis strain BrMgv02-JM63, a chitinolytic bacterium isolated from oil-contaminated mangrove soil in Brazil. Genome Announcements, Washington DC, v. 2, n. 1, p. e01264-13, 2014.Tipo: Artigo em Periódico Indexado | Circulação/Nível: C - 0 |
Biblioteca(s): Embrapa Meio Ambiente. |
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52. | | TAKETANI, N. F.; TAKETANI, R. G.; LEITE, S. G. F.; MELO, I. S. de; LIMA-RIZZO, A. C.; ANDREOTE, F. D.; CUNHA, C. D. da. Effect of nickel in the degradation of oil in soils contaminated with petroleum and nickel. International Journal of Advanced Engineering Research and Science, v. 7, n. 7, p. 511-521, 2020.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Meio Ambiente. |
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53. | | LOPES, L. D; SILVA, L. R. F, da; ROMAGNOLI, E. M; FERREIRA, C.; TAKETANI, R. G.; ABDALLA, A. L.; LOUVANDINI, H.; MENDES, R. Metagenomics of sheep rumen microbiome under two diet. XXI ALAM Congresso Latinoamericano de Microbiologia, Santos-Brasil 28/10/12 a 01/11/2012.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Meio Ambiente. |
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54. | | ANDREOTE, F. D.; DIAS, A. C. F.; FASANELLA, C. C.; TAKETANI, R. G.; ÁVILA, L. A.; PIZZIRANI-KLEINER, A. A.; AZEVEDO, J. L.; MELO, I. S. de. Microbial communities shifting in mangroves under distinct pollution state might name candidates for bioremediation programs. In: WORKSHOP DE BIODEGRADAÇÃO E BIORREMEDIAÇÃO, 3., 2009, Campinas. Anais... Jaguariúna: Embrapa Meio Ambiente, 2009. 1 CD-ROM.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Meio Ambiente. |
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55. | | DURRER, A.; LOPEZ, M. V.; DIAS, A. C. F.; FASANELLA, C. C.; TAKETANI, R. G.; MELO, I. S. de; DINI-ANDREOTE, F. The sulfur-processing community of mangroves under distinct historic of contamination in Brazil. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 26., 2011, Foz do Iguaçu. Anais... Foz do Iguaçu: Sociedade Brasileira de Microbiologia, 2011. Resumo 791-1.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Meio Ambiente. |
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56. | | DURRER, A.; GUMIERE, T.; ANDRADE, P. A. M.; COSTA, D. P.; TAKETANI, R. G.; LIMA, J.; SILVA, M. P.; MELO, I. S. de; ANDREOTE, F. D. Soils properties outpaces agricultural practices in the determination of bacterial community structure in sugarcane fields. In: INTERNATIONAL SYMPOSIUM ON MICROBIAL ECOLOGY, 15., 2014, Seoul. Proceedings... Wageningen: The International Society for Microbial Ecology (ISME), 2014. p. 385-386.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Meio Ambiente. |
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57. | | CASTELIANI, A. G. B.; KAVAMURA, V. N.; ZUCCHI, T. D.; SÁBER, M. L.; NASCIMENTO, R. dos S.; FRIGHETTO, R. T. S.; TAKETANI, R. G.; MELO, I. S. de. UV-B resistant yeast inhabit the phyllosphere of strawberry. British Microbiology Research Journal, London, v. 4, n. 10, p. 1105-1117, 2014.Tipo: Artigo em Periódico Indexado | Circulação/Nível: C - 0 |
Biblioteca(s): Embrapa Meio Ambiente. |
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58. | | ROMAGNOLI, E. M.; NATEL, A. S.; FAGUNDES, G. G.; SANTOS, J. E.; SANTOS, P. P. dos; CAMPOS, F. C. de; FERREIRA, C; TAKETANI, R. G.; LOPES, L. D.; MENDES, R. Quantifying cellulolytic bacteria in the rumen of sheep under a diet with sugarcane bagasse. In: SYMPOSIUM ON BACTERIAL GENETICS AND ECOLOGY, 12., 2013, Ljubljana (Slovenia). Networking and plasticity of microbial communities: the secret to success. Ljubljana: University of Ljubljana. 2013. p. 105. Ref. P130.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Meio Ambiente. |
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59. | | ANDREOTE, F. D.; JIMENEZ, D. J.; CHAVES, D.; DIAS, A. C. F.; LUVIZOTTO, D. M.; DINI-ANDREOTE, F.; FASANELLA, C. C.; VARON LOPEZ, M.; BAENA, S.; TAKETANI, R. G.; MELO, I. S. de. The microbiome of brazilian mangrove sediments as revealed by metagenomics. Plos One, v. 7, n. 6, 14 p., 2012.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Meio Ambiente. |
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60. | | KAVAMURA, V.; SANTOS, S. N.; SILVA, J. L. da; PARMA, M. M.; AVILA, L. A.; VISCONTI, A.; ZUCCHI, T. D.; TAKETANI, R. G.; ANDREOTE, F. D.; MELO, I. S. de. Screening of Brazilian cacti rhizobacteria for plant growth promotion under drought. Microbiological Research, Jena, v. 168, n. 4, p. 183-191, 2013.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Meio Ambiente. |
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Registros recuperados : 63 | |
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Nenhum registro encontrado para a expressão de busca informada. |
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