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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
20/05/1993 |
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Data da última atualização: |
20/05/1993 |
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Autoria: |
SU H.; LIN, C. |
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Afiliação: |
Department of Plant Pathology & Entomology, National Taiwan University, Taipei, Taiwan, China. |
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Título: |
Yolk media for cultivation of spiroplasmas. |
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Ano de publicação: |
1981 |
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Fonte/Imprenta: |
Tokyo, Japan: International Society of Citricultural, 1981. |
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Volume: |
v.1 |
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Páginas: |
p.458-460 |
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Idioma: |
Inglês |
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Conteúdo: |
Citrus stubborn spiroplasma (S. citri) and honeybee spiroplasma grew favorably in C-3G basic medium containing 5% yolk fluid or 10% amniotic fluid. S. citri, honeybee spiroplasma (HB-24), and flower spiroplasma (SR-3) grew favorably in yolk media formulated by replacing the horse serum constituent of commonly used serum media with 0.6-5.0% chiken egg yolk fluid. The serum constituent in serum media could be substituted by yolk, but yolk could not substitute for PPLO broth or sucrose. Yolk solution clarified by heating at 56 graus for 30 min, centrifuging at 10,000 rpm for 30 min, and membrane (0.45 micron) filtering, was slightly better for growth than unclarified yolk solution which hindered microscopic observation. S. citri grew better in a media with low yolk content (1.25-0.65%), while HB-24 and corn-stunt spiroplasmas grew better in 2.5-5.0% yolk medium formulated with phosphate buffer solution (0.01M) at pH 7.2-7.6. SR-3 grew equally well in any medium containing over 0.65% yolk. The yolk media were much cheaper than serum media. |
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Palavras-Chave: |
Flower spiroplasma; Honeybee spiroplasma; Stubborn. |
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Thesaurus Nal: |
Spiroplasma citri. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01574naa a2200205 a 4500
001 1644021
005 1993-05-20
008 1981 bl uuuu u00u1 u #d
100 1 $aSU H.
245 $aYolk media for cultivation of spiroplasmas.
260 $c1981
300 $ap.458-460 v.1
490 $vv.1
520 $aCitrus stubborn spiroplasma (S. citri) and honeybee spiroplasma grew favorably in C-3G basic medium containing 5% yolk fluid or 10% amniotic fluid. S. citri, honeybee spiroplasma (HB-24), and flower spiroplasma (SR-3) grew favorably in yolk media formulated by replacing the horse serum constituent of commonly used serum media with 0.6-5.0% chiken egg yolk fluid. The serum constituent in serum media could be substituted by yolk, but yolk could not substitute for PPLO broth or sucrose. Yolk solution clarified by heating at 56 graus for 30 min, centrifuging at 10,000 rpm for 30 min, and membrane (0.45 micron) filtering, was slightly better for growth than unclarified yolk solution which hindered microscopic observation. S. citri grew better in a media with low yolk content (1.25-0.65%), while HB-24 and corn-stunt spiroplasmas grew better in 2.5-5.0% yolk medium formulated with phosphate buffer solution (0.01M) at pH 7.2-7.6. SR-3 grew equally well in any medium containing over 0.65% yolk. The yolk media were much cheaper than serum media.
650 $aSpiroplasma citri
653 $aFlower spiroplasma
653 $aHoneybee spiroplasma
653 $aStubborn
700 1 $aLIN, C.
773 $tTokyo, Japan: International Society of Citricultural, 1981.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 4 | |
| 1. |  | PÖHLKER, M. L.; PÖHLKER, C.; DITAS, F.; KLIMACH, T.; ANGELIS, I. H. de; ARAUJO, A. C. de; BRITO, J.; CARBONE, S.; CHENG, Y.; CHI, X.; DITZ, R.; GUNTHE, S. S.; KESSELMEIER, J.; KÖNEMANN, T.; LAVRIC, J. V.; MARTIN, S. T.; MIKHAILOV, E.; MORAN-ZULOAGA, D.; ROSE, D.; SATURNO, J.; SU, H.; THALMAN, R.; WALTER, D.; WANG, J.; WOLFF, S.; BARBOSA, H. M. J.; ARTAXO, P.; ANDREAE, M. O.; PÖSCHL, U. Long-term observations of cloud condensation nuclei in the Amazon rain forest - Part 1: Aerosol size distribution, hygroscopicity, and new model parametrizations for CCN prediction. Atmospheric Chemistry and Physics, v. 16, n. 24, p. 15709-15740, Dec. 2016. Na publicação: Araújo, A.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Oriental. |
|    |
| 2. | | PÖHLKER, M. L.; DITAS, F.; SATURNO, J.; KLIMACH, T.; ANGELIS, I. H. de; ARAUJO, A. C. de; BRITO, J.; CARBONE, S.; CHENG, Y.; CHI, X.; DITZ, R.; GUNTHE, S. S.; HOLANDA, B. A.; KANDLER, K.; KESSELMEIER, J.; KÖNEMANN, T.; KRÜGER, O. O.; LAVRIC, J. V.; MARTINS, S. T.; MIKHAILOV, E.; MORAN-ZULOAGA, D.; RIZZO, L. V.; ROSE, D.; SU, H.; THALMAN, R.; WALTER, D.; WANG, J.; WOLFF, S.; BARBOSA, H. M. J.; ARTAXO, P.; ANDREAE, M. O.; PÖSCHL, U.; PÖHLKER, C. Long-term observations of cloud condensation nuclei over the Amazon rain forest - Part 2: Variability and characteristics of biomass burning, long-range transport, and pristine rain forest aerosols. Atmospheric Chemistry and Physics, v. 18, n. 14, p. 10289-10331, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Oriental. |
|  |
| 3. | | ANDREAE, M. O.; ACEVEDO, O. C.; ARAUJO, A.; ARTAXO, P.; BARBOSA, C. G. G.; BARBOSA, H. M. J.; BRITO, J.; CARBONE, S.; CHI, X.; CINTRA, B. B. L.; SILVA, N. F. da; DIAS, N. L.; DIAS-JÚNIOR, C. Q.; DITAS, F.; DITZ, R.; GODOI, A. F. L.; GODOI, R. H. M.; HEIMANN, M.; HOFFMANN, T.; KESSELMEIER, J.; KÖNEMANN, T.; KRÜGER, M. L.; LAVRIC, J. V.; MANZI, A. O.; MORAN-ZULOAGA, D.; NÖLSCHER, A. C.; NOGUEIRA, D. S.; PIEDADE, M. T. F.; PÖHLKER, C.; PÖSCHL, U.; RIZZO, L. V.; RO, C.-U.; RUCKTESCHLER, N.; SÁ, L. D. A.; SÁ, M. D. O.; SALES, C. B.; SANTOS, R. M. N. dos; SATURNO, J.; SCHÖNGART, J.; SÖRGEL, M.; SOUZA, C. M. de; SOUZA, R. A. F. de; SU, H.; TARGHETTA, N.; TÓTA, J.; TREBS, I.; TRUMBORE, S.; EIJCK, A. van; WALTER, D.; WANG, Z.; WEBER, B.; WILLIAMS, J.; WINDERLICH, J.; WITTMANN, F.; WOLFF, S.; YÁÑEZ-SERRANO, A. M. The Amazon Tall Tower Observatory (ATTO) in the remote Amazon basin: overview of first results from ecosystem ecology, meteorology, trace gas, and aerosol measurements. Atmospheric Chemistry and Physics Discuss, v. 15, n. 18, p. 11599-11726, 2015.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 4. | | ANDREAE, M. O.; ACEVEDO, O. C.; ARAUJO, A.; ARTAXO, P.; BARBOSA, C. G. G.; BARBOSA, H. M. J.; BRITO, J.; CARBONE, S.; CHI, X.; CINTRA, B. B. L.; SILVA, N. F. da; DIAS, N. L.; DIAS-JÚNIOR, C. Q.; DITAS, F.; DITZ, R.; GODOI, A. F. L.; GODOI, R. H. M.; HEIMANN, M.; HOFFMANN, T.; KESSELMEIER, J.; KÖNEMANN, T.; KRÜGER, M. L.; LAVRIC, J. V.; MANZI, A. O.; LOPES, A. P.; MARTINS, D. L.; MIKHAILOV, E. F.; MORAN-ZULOAGA, D.; NELSON, B. W.; NÖLSCHER, A. C.; NOGUEIRA, D. S.; PIEDADE, M. T. F.; PÖHLKER, C.; PÖSCHL, U.; QUESADA, C. A.; RIZZO, L. V.; RO, C.-U.; RUCKTESCHLER, N.; SÁ, L. D. A.; SÁ, M. de O.; SALES, C. B.; SANTOS, R. M. N. dos; SATURNO, J.; SCHÖNGART, J.; SÖRGEL, M.; SOUZA, C. M. de; SOUZA, R. A. F. de; SU, H.; TARGHETTA, N.; TÓTA, J.; TREBS, I.; TRUMBORE, S.; EIJCK, A. van; WALTER, D.; WANG, Z.; WEBER, B.; WILLIAMS, J.; WINDERLICH, J.; WITTMANN, F.; WOLFF, S.; YÁÑEZ-SERRANO, A. M. The Amazon Tall Tower Observatory (ATTO): overview of pilot measurements on ecosystem ecology, meteorology, trace gases, and aerosols. Atmospheric Chemistry and Physics, v. 15, n. 18, p. 10723-10776, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Oriental. |
| |
| Registros recuperados : 4 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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