01574naa a2200205 a 450000100080000000500110000800800410001910000100006024500480007026000090011830000180012749000080014552010560015365000220120965300230123165300250125465300130127970000120129277300640130416440211993-05-20       1981    bl uuuu     u00u1 u    #d1 aSU H.  aYolk media for cultivation of spiroplasmas.  c1981  ap.458-460 v.1  vv.1  aCitrus stubborn spiroplasma (S. citri) and honeybee spiroplasma grew favorably in C-3G basic medium containing 5% yolk fluid or 10% amniotic fluid. S. citri, honeybee spiroplasma (HB-24), and flower spiroplasma (SR-3) grew favorably in yolk media formulated by replacing the horse serum constituent of commonly used serum media with 0.6-5.0% chiken egg yolk fluid. The serum constituent in serum media could be substituted by yolk, but yolk could not substitute for PPLO broth or sucrose. Yolk solution clarified by heating at 56 graus for 30 min, centrifuging at 10,000 rpm for 30 min, and membrane (0.45 micron) filtering, was slightly better for growth than unclarified yolk solution which hindered microscopic observation. S. citri grew better in a media with low yolk content (1.25-0.65%), while HB-24 and corn-stunt spiroplasmas grew better in 2.5-5.0% yolk medium formulated with phosphate buffer solution (0.01M) at pH 7.2-7.6. SR-3 grew equally well in any medium containing over 0.65% yolk. The yolk media were much cheaper than serum media.  aSpiroplasma citri  aFlower spiroplasma  aHoneybee spiroplasma  aStubborn1 aLIN, C.  tTokyo, Japan: International Society of Citricultural, 1981.