| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Acre. |
|
Data corrente: |
22/09/2020 |
|
Data da última atualização: |
28/06/2021 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
OLIVEIRA, J. C. de; SILVA, C. C. da; SOUZA, A. P. de; CAMPOS, T. de. |
|
Afiliação: |
Jônatas Chagas de Oliveira, Universidade Federal do Acre (Ufac); Carla Cristina da Silva, Universidade Estadual de Campinas (Unicamp); Anete Pereira de Souza, Universidade Estadual de Campinas (Unicamp); TATIANA DE CAMPOS, CPAF-AC. |
|
Título: |
A Modified protocol for total RNA isolation from forage peanuts. |
|
Ano de publicação: |
2020 |
|
Fonte/Imprenta: |
South American Journal of Basic Education, Technical and Technological, v. 7, n. 1, p. 683-687, jan./abr. 2020. |
|
ISSN: |
2446-4821 |
|
Idioma: |
Inglês |
|
Conteúdo: |
The development of specific protocols for extraction of high-quality RNA are key advances in molecular techniques for elucidating biological processes and mechanisms. The forage peanut has significant economic value for use in mixed pastures. However, it is difficult to extract high-quality RNA from forage peanuts due to high contents of tannins, polysaccharides, and other secondary metabolites. Here, it is described an efficient method for obtaining high-quality, high-yield total RNA that is usable in downstream transcriptome analysis. This modifications helped eliminate the problems associated with the presence of high concentrations of secondary metabolites in forage peanut leaves, and lacked signs of degradation. O desenvolvimento de protocolos específicos para extração de RNA de alta qualidade são avanços chave em técnicas moleculares para elucidar mecanismos e processos biológicos. O amendoim forrageiro tem significante valor econômico para uso em pastagens consorciadas. Entretanto, é difícil extrair RNA de alta qualidade de amendoim forrageiro devido aos altos conteúdos de taninos, polissacarídeos e outros metabólitos secundários. Aqui, descreve-se um eficiente método para obtenção de RNA total de alta qualidade e alto rendimento que é necessário para análises posteriores do transcriptoma. Essas modificações ajudaram a eliminar os problemas associados com a presença de altas concentrações de metabólitos secundários em folhas de amendoim forrageiro e sem sinais de degradação. MenosThe development of specific protocols for extraction of high-quality RNA are key advances in molecular techniques for elucidating biological processes and mechanisms. The forage peanut has significant economic value for use in mixed pastures. However, it is difficult to extract high-quality RNA from forage peanuts due to high contents of tannins, polysaccharides, and other secondary metabolites. Here, it is described an efficient method for obtaining high-quality, high-yield total RNA that is usable in downstream transcriptome analysis. This modifications helped eliminate the problems associated with the presence of high concentrations of secondary metabolites in forage peanut leaves, and lacked signs of degradation. O desenvolvimento de protocolos específicos para extração de RNA de alta qualidade são avanços chave em técnicas moleculares para elucidar mecanismos e processos biológicos. O amendoim forrageiro tem significante valor econômico para uso em pastagens consorciadas. Entretanto, é difícil extrair RNA de alta qualidade de amendoim forrageiro devido aos altos conteúdos de taninos, polissacarídeos e outros metabólitos secundários. Aqui, descreve-se um eficiente método para obtenção de RNA total de alta qualidade e alto rendimento que é necessário para análises posteriores do transcriptoma. Essas modificações ajudaram a eliminar os problemas associados com a presença de altas concentrações de metabólitos secundários em folhas de amendoim forrageiro e sem sinais de degr... Mostrar Tudo |
|
Palavras-Chave: |
Ácido ribonucleico; Alta calidad; Amendoim forrageiro; Cacahuetes forrajeros; Extracción de ARN; Forage peanut; High quality; Metabólico secundário; RNA extraction; Secondary metabolic. |
|
Thesagro: |
Extração; Leguminosa Forrageira; Melhoramento Genético Vegetal; Qualidade; Rendimento; RNA. |
|
Thesaurus Nal: |
Arachis pintoi; Forage legumes; Plant breeding. |
|
Categoria do assunto: |
G Melhoramento Genético |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/216147/1/27028.pdf
|
|
Marc: |
LEADER 02700naa a2200397 a 4500
001 2125058
005 2021-06-28
008 2020 bl uuuu u00u1 u #d
022 $a2446-4821
100 1 $aOLIVEIRA, J. C. de
245 $aA Modified protocol for total RNA isolation from forage peanuts.$h[electronic resource]
260 $c2020
520 $aThe development of specific protocols for extraction of high-quality RNA are key advances in molecular techniques for elucidating biological processes and mechanisms. The forage peanut has significant economic value for use in mixed pastures. However, it is difficult to extract high-quality RNA from forage peanuts due to high contents of tannins, polysaccharides, and other secondary metabolites. Here, it is described an efficient method for obtaining high-quality, high-yield total RNA that is usable in downstream transcriptome analysis. This modifications helped eliminate the problems associated with the presence of high concentrations of secondary metabolites in forage peanut leaves, and lacked signs of degradation. O desenvolvimento de protocolos específicos para extração de RNA de alta qualidade são avanços chave em técnicas moleculares para elucidar mecanismos e processos biológicos. O amendoim forrageiro tem significante valor econômico para uso em pastagens consorciadas. Entretanto, é difícil extrair RNA de alta qualidade de amendoim forrageiro devido aos altos conteúdos de taninos, polissacarídeos e outros metabólitos secundários. Aqui, descreve-se um eficiente método para obtenção de RNA total de alta qualidade e alto rendimento que é necessário para análises posteriores do transcriptoma. Essas modificações ajudaram a eliminar os problemas associados com a presença de altas concentrações de metabólitos secundários em folhas de amendoim forrageiro e sem sinais de degradação.
650 $aArachis pintoi
650 $aForage legumes
650 $aPlant breeding
650 $aExtração
650 $aLeguminosa Forrageira
650 $aMelhoramento Genético Vegetal
650 $aQualidade
650 $aRendimento
650 $aRNA
653 $aÁcido ribonucleico
653 $aAlta calidad
653 $aAmendoim forrageiro
653 $aCacahuetes forrajeros
653 $aExtracción de ARN
653 $aForage peanut
653 $aHigh quality
653 $aMetabólico secundário
653 $aRNA extraction
653 $aSecondary metabolic
700 1 $aSILVA, C. C. da
700 1 $aSOUZA, A. P. de
700 1 $aCAMPOS, T. de
773 $tSouth American Journal of Basic Education, Technical and Technological$gv. 7, n. 1, p. 683-687, jan./abr. 2020.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Acre (CPAF-AC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Gado de Leite. |
|
Data corrente: |
02/12/2014 |
|
Data da última atualização: |
05/02/2024 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 2 |
|
Autoria: |
HMELJEVSKIA, K. V.; FREITAS, L.; DOMINGUES, R.; PEREIRA, A. R.; CANCIO, A. S.; ANDRADE, A. C. S.; MACHADO, M. A.; VICCINI, L. F.; FORZZA, R. C. |
|
Afiliação: |
KARINA V. HMELJEVSKIA, UFRJ; LEANDRO FREITAS, Jardim Botânico do Rio de Janeiro; ROBERT DOMINGUES, UFJF; ALBA REGINA PEREIRA, Centro Federal de Educaçãoo Tecnológica Celso Suckow da Fonseca; ALINE S. CANCIO, Jardim Botânico do Rio de Janeiro; ANTONIO CARLOS S. ANDRADE, Jardim Botânico do Rio de Janeiro; MARCO ANTONIO MACHADO, CNPGL; LYDERSON F. VICCINI, UFJF; RAFAELA C. FORZZA, Jardim Botânico do Rio de Janeiro. |
|
Título: |
Conservation assessment of an extremely restricted bromeliad highlights the need for population-based conservation on granitic inselbergs of the Brazilian Atlantic Forest. |
|
Ano de publicação: |
2014 |
|
Fonte/Imprenta: |
Flora, n. 209, n. 5/6, p. 250-259, 2014. |
|
DOI: |
https://doi.org/10.1016/j.flora.2014.03.004 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Inselbergs support highly specialized vegetation with many endemic species. Here we studied by an integrated approach a rare and threatened species from Brazilian inselbergs. Pitcairnia encholirioides (Bromeliaceae) is included in the Red Book of Brazilian Flora, in the ‘Endangered’ category. Only one single population on unprotected private property was known at the beginning of our study. We studied several life-history traits including clonal growth, reproductive biology, seed germination and cryopreservation, and genetic diversity. Pitcairnia encholirioides has the phalanx type of clonal growth strategy. Its self-incompatible flowers are successfully pollinated by hummingbirds. However, less than 5% of clonal fragments produced flowers. Seeds can be wind-dispersed, but most of them are dispersed by gravity and fall close to the mother plant. The percentage and speed of germination were significantly affected by different temperatures, water potential, and red:far-red light ratios, although more than 80% of seeds germinated under optimal physiological conditions. The genetic diversity was very low (Hsp = 0.09) and the population was highly structured (ΦST = 0.51). The combination of dispersal mode and environmental conditions seems to be the main factor leading to the rarity of P. encholiroides. Low genetic diversity combined with small population size and low reproductive capacity suggest that the species is highly susceptible to environmental disturbance. Similarly to P. encholiroides, many species from Brazilian inselbergs are restricted to only a few localities. For more widely distributed species, a higher genetic diversity among populations has been consistently detected. The pattern of low gene flow of many species with small distributions highlight the importance of population-based conservation strategies for such taxa the areas of occurrence of which being naturally fragmented at inselberg sites. We discuss some strategies that may help in conservation of such species. MenosInselbergs support highly specialized vegetation with many endemic species. Here we studied by an integrated approach a rare and threatened species from Brazilian inselbergs. Pitcairnia encholirioides (Bromeliaceae) is included in the Red Book of Brazilian Flora, in the ‘Endangered’ category. Only one single population on unprotected private property was known at the beginning of our study. We studied several life-history traits including clonal growth, reproductive biology, seed germination and cryopreservation, and genetic diversity. Pitcairnia encholirioides has the phalanx type of clonal growth strategy. Its self-incompatible flowers are successfully pollinated by hummingbirds. However, less than 5% of clonal fragments produced flowers. Seeds can be wind-dispersed, but most of them are dispersed by gravity and fall close to the mother plant. The percentage and speed of germination were significantly affected by different temperatures, water potential, and red:far-red light ratios, although more than 80% of seeds germinated under optimal physiological conditions. The genetic diversity was very low (Hsp = 0.09) and the population was highly structured (ΦST = 0.51). The combination of dispersal mode and environmental conditions seems to be the main factor leading to the rarity of P. encholiroides. Low genetic diversity combined with small population size and low reproductive capacity suggest that the species is highly susceptible to environmental disturbance. Similarly to P... Mostrar Tudo |
|
Palavras-Chave: |
Clonal plants; Endemism; Rarity. |
|
Thesaurus NAL: |
endangered species; ex situ conservation; in situ conservation. |
|
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
|
Marc: |
LEADER 02981naa a2200301 a 4500
001 2001285
005 2024-02-05
008 2014 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.flora.2014.03.004$2DOI
100 1 $aHMELJEVSKIA, K. V.
245 $aConservation assessment of an extremely restricted bromeliad highlights the need for population-based conservation on granitic inselbergs of the Brazilian Atlantic Forest.$h[electronic resource]
260 $c2014
520 $aInselbergs support highly specialized vegetation with many endemic species. Here we studied by an integrated approach a rare and threatened species from Brazilian inselbergs. Pitcairnia encholirioides (Bromeliaceae) is included in the Red Book of Brazilian Flora, in the ‘Endangered’ category. Only one single population on unprotected private property was known at the beginning of our study. We studied several life-history traits including clonal growth, reproductive biology, seed germination and cryopreservation, and genetic diversity. Pitcairnia encholirioides has the phalanx type of clonal growth strategy. Its self-incompatible flowers are successfully pollinated by hummingbirds. However, less than 5% of clonal fragments produced flowers. Seeds can be wind-dispersed, but most of them are dispersed by gravity and fall close to the mother plant. The percentage and speed of germination were significantly affected by different temperatures, water potential, and red:far-red light ratios, although more than 80% of seeds germinated under optimal physiological conditions. The genetic diversity was very low (Hsp = 0.09) and the population was highly structured (ΦST = 0.51). The combination of dispersal mode and environmental conditions seems to be the main factor leading to the rarity of P. encholiroides. Low genetic diversity combined with small population size and low reproductive capacity suggest that the species is highly susceptible to environmental disturbance. Similarly to P. encholiroides, many species from Brazilian inselbergs are restricted to only a few localities. For more widely distributed species, a higher genetic diversity among populations has been consistently detected. The pattern of low gene flow of many species with small distributions highlight the importance of population-based conservation strategies for such taxa the areas of occurrence of which being naturally fragmented at inselberg sites. We discuss some strategies that may help in conservation of such species.
650 $aendangered species
650 $aex situ conservation
650 $ain situ conservation
653 $aClonal plants
653 $aEndemism
653 $aRarity
700 1 $aFREITAS, L.
700 1 $aDOMINGUES, R.
700 1 $aPEREIRA, A. R.
700 1 $aCANCIO, A. S.
700 1 $aANDRADE, A. C. S.
700 1 $aMACHADO, M. A.
700 1 $aVICCINI, L. F.
700 1 $aFORZZA, R. C.
773 $tFlora$gn. 209, n. 5/6, p. 250-259, 2014.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
