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 | Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
11/08/2023 |
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Data da última atualização: |
15/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, E. L. dos; BESPALHOK FILHO, J. C.; HANSEL, F. A.; SOUSA, J. A. T.; AUER, C. G.; STEINER, N.; DEGENHARDT, J. |
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Afiliação: |
EVELYN LOPES DOS SANTOS, Federal University of Paraná; JOÃO CARLOS BESPALHOK FILHO, Federal University of Paraná; FABRICIO AUGUSTO HANSEL, CNPF; JOÃO ARTHUR TIKLER SOUSA, Federal University of Paraná; CELSO GARCIA AUER, CNPF; NEUSA STEINER, Federal University of Santa Catarina; JULIANA DEGENHARDT GOLDBACH, CNPF. |
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Título: |
Azacytidine affects gene expression and metabolic profile of Pinus elliottii x Pinus caribaea var. hondurensis embryogenic cell lines. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Plant Cell, Tissue and Organ Culture, v. 155, p. 637-651, 2023. |
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DOI: |
https://doi.org/10.1007/s11240-023-02584-6 |
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Idioma: |
Português |
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Conteúdo: |
The current study evaluated the effect of (i) genotypes on somatic embryogenesis initiation of Pinus elliottii x Pinus caribaea var. hondurensis and (ii) 5-azacytidine (5-azaC), a potential agent for embryogenic competence reacquisition. For the establishment of embryogenic cell lines (ECLs), immature megagametophytes of thirteen families were cultured on an initiation medium. After 17 months, two aged ECLs (ECL02 and ECL05) were exposed for 10 days to 5-azaC (0, 50 and 150 ?M). Gene expression analysis of Somatic Embryogenesis Receptor Kinase 1 (SERK1) and Leafy Cotyledon 1 (LEC1) were carried out by RT-qPCR in both ECLs. Metabolomic analysis via GC?MS were performed in the aged ECL05 and in a young ECL. ECLs initiation was genotype-dependent. The aged ECL05 exposed to 150 µM 5-azaC showed an increase in SERK1 and LEC1 expressions in comparison to the control and a similar metabolic profile to the young ECL. It suggests that 5-azaC has an effect on embryogenic morphology of the aged ECL05. On the other hand, it seems that the response to 5-azaC is also affected by the genotype, as the expression of both genes showed an opposite pattern in the ECL02. |
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Palavras-Chave: |
Embryogenic potential; Epigenética. |
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Thesagro: |
Biotecnologia; Embriogénese; Pinus Caribaea; Pinus Elliottii. |
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Thesaurus Nal: |
Biotechnology; DNA methylation; Epigenetics; Somatic embryos. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02181naa a2200325 a 4500
001 2155864
005 2024-02-15
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s11240-023-02584-6$2DOI
100 1 $aSANTOS, E. L. dos
245 $aAzacytidine affects gene expression and metabolic profile of Pinus elliottii x Pinus caribaea var. hondurensis embryogenic cell lines.$h[electronic resource]
260 $c2023
520 $aThe current study evaluated the effect of (i) genotypes on somatic embryogenesis initiation of Pinus elliottii x Pinus caribaea var. hondurensis and (ii) 5-azacytidine (5-azaC), a potential agent for embryogenic competence reacquisition. For the establishment of embryogenic cell lines (ECLs), immature megagametophytes of thirteen families were cultured on an initiation medium. After 17 months, two aged ECLs (ECL02 and ECL05) were exposed for 10 days to 5-azaC (0, 50 and 150 ?M). Gene expression analysis of Somatic Embryogenesis Receptor Kinase 1 (SERK1) and Leafy Cotyledon 1 (LEC1) were carried out by RT-qPCR in both ECLs. Metabolomic analysis via GC?MS were performed in the aged ECL05 and in a young ECL. ECLs initiation was genotype-dependent. The aged ECL05 exposed to 150 µM 5-azaC showed an increase in SERK1 and LEC1 expressions in comparison to the control and a similar metabolic profile to the young ECL. It suggests that 5-azaC has an effect on embryogenic morphology of the aged ECL05. On the other hand, it seems that the response to 5-azaC is also affected by the genotype, as the expression of both genes showed an opposite pattern in the ECL02.
650 $aBiotechnology
650 $aDNA methylation
650 $aEpigenetics
650 $aSomatic embryos
650 $aBiotecnologia
650 $aEmbriogénese
650 $aPinus Caribaea
650 $aPinus Elliottii
653 $aEmbryogenic potential
653 $aEpigenética
700 1 $aBESPALHOK FILHO, J. C.
700 1 $aHANSEL, F. A.
700 1 $aSOUSA, J. A. T.
700 1 $aAUER, C. G.
700 1 $aSTEINER, N.
700 1 $aDEGENHARDT, J.
773 $tPlant Cell, Tissue and Organ Culture$gv. 155, p. 637-651, 2023.
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Embrapa Florestas (CNPF) |
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| Registros recuperados : 7 | |
| 5. |  | SANTOS, E. L. dos; BESPALHOK FILHO, J. C.; HANSEL, F. A.; SOUSA, J. A. T.; AUER, C. G.; STEINER, N.; DEGENHARDT, J. Azacytidine affects gene expression and metabolic profile of Pinus elliottii x Pinus caribaea var. hondurensis embryogenic cell lines. Plant Cell, Tissue and Organ Culture, v. 155, p. 637-651, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Florestas. |
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| 6. | | RODRIGUES, V. de S.; CARVALHO, G. M. C.; BARROS, D. A.; CAVALCANTE, M. M. A. de S.; SILVA, J. H. L. da; SOUSA, J. A. T. de. Características histomorfológicas testiculares de touros curraleiro pé-duro, nelore e cruzamentos. Revista RG News, v. 4, n. 3, p. 42, 2018. Edição especial dos Anais do V Congresso Brasileiro de Recursos Genéticos, Fortaleza, nov. 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 7. | | OLIVEIRA, P. F. N. M. de; COSTA, A. P. R.; AZEVEDO, D. M. M. R.; SOUSA, J. A. T. de; SOUSA JUNIOR, A. de; SILVA, E. L. L. da; MURATORI, M. C. S. Superovulação em cabras SDR com FSH-p e somatotrofina bovina recombinante. Revista Científica de Produção Animal, Fortaleza, v. 8, n. 1, p. 73-81, jan./jun. 2006.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - B |
| Biblioteca(s): Embrapa Meio-Norte. |
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| Registros recuperados : 7 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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