02181naa a2200325 a 450000100080000000500110000800800410001902400520006010000220011224501620013426000090029652011740030565000180147965000200149765000160151765000200153365000180155365000180157165000190158965000200160865300260162865300170165470000270167170000180169870000200171670000160173670000160175270000190176877300680178721558642024-02-15       2023    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1007/s11240-023-02584-62DOI1 aSANTOS, E. L. dos  aAzacytidine affects gene expression and metabolic profile of Pinus elliottii x Pinus caribaea var. hondurensis embryogenic cell lines.h[electronic resource]  c2023  aThe current study evaluated the effect of (i) genotypes on somatic embryogenesis initiation of Pinus elliottii x Pinus caribaea var. hondurensis and (ii) 5-azacytidine (5-azaC), a potential agent for embryogenic competence reacquisition. For the establishment of embryogenic cell lines (ECLs), immature megagametophytes of thirteen families were cultured on an initiation medium. After 17 months, two aged ECLs (ECL02 and ECL05) were exposed for 10 days to 5-azaC (0, 50 and 150 ?M). Gene expression analysis of Somatic Embryogenesis Receptor Kinase 1 (SERK1) and Leafy Cotyledon 1 (LEC1) were carried out by RT-qPCR in both ECLs. Metabolomic analysis via GC?MS were performed in the aged ECL05 and in a young ECL. ECLs initiation was genotype-dependent. The aged ECL05 exposed to 150 µM 5-azaC showed an increase in SERK1 and LEC1 expressions in comparison to the control and a similar metabolic profile to the young ECL. It suggests that 5-azaC has an effect on embryogenic morphology of the aged ECL05. On the other hand, it seems that the response to 5-azaC is also affected by the genotype, as the expression of both genes showed an opposite pattern in the ECL02.  aBiotechnology  aDNA methylation  aEpigenetics  aSomatic embryos  aBiotecnologia  aEmbriogénese  aPinus Caribaea  aPinus Elliottii  aEmbryogenic potential  aEpigenética1 aBESPALHOK FILHO, J. C.1 aHANSEL, F. A.1 aSOUSA, J. A. T.1 aAUER, C. G.1 aSTEINER, N.1 aDEGENHARDT, J.  tPlant Cell, Tissue and Organ Culturegv. 155, p. 637-651, 2023.