|
|
Registro Completo |
Biblioteca(s): |
Embrapa Cerrados. |
Data corrente: |
11/02/1993 |
Data da última atualização: |
11/02/1993 |
Autoria: |
HUTTON, E. M.; SOUSA, F. B. de. |
Título: |
Collecting and breeding legumes for brazilian tropical pastures. |
Ano de publicação: |
1985 |
Fonte/Imprenta: |
In: INTERNATIONAL GRASSLAND CONGRESS, 15., 1985, Kyoto, Japan. Proceedings. Nishi-Nasuno: The Science Council of Japan / The Japanese Society of Grassland Science, 1985. |
Páginas: |
p.217-219. |
Idioma: |
Português |
Palavras-Chave: |
Brasil; Coleta; Planta Graminea Forrageira; Planta Leguminosa Forrageira; Regiao Tropical. |
Thesagro: |
Centrosema; Cerrado; Espécie; Zornia. |
Thesaurus Nal: |
Stylosanthes. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00793naa a2200253 a 4500 001 1547842 005 1993-02-11 008 1985 bl uuuu u00u1 u #d 100 1 $aHUTTON, E. M. 245 $aCollecting and breeding legumes for brazilian tropical pastures. 260 $c1985 300 $ap.217-219. 650 $aStylosanthes 650 $aCentrosema 650 $aCerrado 650 $aEspécie 650 $aZornia 653 $aBrasil 653 $aColeta 653 $aPlanta Graminea Forrageira 653 $aPlanta Leguminosa Forrageira 653 $aRegiao Tropical 700 1 $aSOUSA, F. B. de 773 $tIn: INTERNATIONAL GRASSLAND CONGRESS, 15., 1985, Kyoto, Japan. Proceedings. Nishi-Nasuno: The Science Council of Japan / The Japanese Society of Grassland Science, 1985.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Cerrados (CPAC) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
26/07/2018 |
Data da última atualização: |
26/07/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
CARVALHO, A. L. de A.; REZENDE, L. C. de; COSTA, L. B.; HALFELD-VIEIRA, B. de A.; PINTO, Z. V.; MORANDI, M. A. B.; MEDEIROS, F. H. V.; BETTIOL, W. |
Afiliação: |
ANDRE LUIZ DE ANDRADE CARVALHO, UFV; LARISSA CASTRO DE REZENDE, UFV; LUCIO BERTOLDO COSTA, FCA-UNESP; BERNARDO DE ALMEIDA HALFELD VIEIRA, CNPMA; ZAYAME VEGETTE PINTO, FCA-UNESP; MARCELO AUGUSTO BOECHAT MORANDI, CNPMA; FLAVIO HENRIQUE VASCONCELOS DE MEDEIROS, UFV; WAGNER BETTIOL, CNPMA. |
Título: |
Optimizing the mass production of Clonostachys rosea by liquid-state fermentation. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Biological Control, v. 118, p. 16-25, 2018. |
DOI: |
https://doi.org/10.1016/j.biocontrol.2017.11.014 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: Optimization of the culture medium and the entire mass production process for various fungi in liquid medium has been studied. However, the technology is not well developed for Clonostachys rosea, a biocontrol agent against various plant pathogens due to its antagonistic capacity to act as a hyperparasite, compete for nutrients and space, and induce plant resistance to pathogens. In this study, we aimed to optimize the culture medium and to standardize parameters that may interfere with the production of C. rosea conidia in a liquid-state fermentation system. Culturing was performed in 250-mL Erlenmeyer flasks shaken for 7 days, followed by planned experimental methodology to reduce the number of analyses and consumable costs. Benchtop bioreactor tests with the optimized medium were performed. Glucose and sucrose were evaluated as carbon sources. Initially, the effects of temperature, pH, photoperiod, carbon:nitrogen ratio and water activity on inoculum production were evaluated, with the pH and photoperiod being factors that contributed to conidial production. Optimization of the fermentation conditions was performed using a central composite rotational design (CCD) with a wider range of pH values and photoperiods. The remaining variables were fixed according to the previous assay. Colony-forming unit (CFUs), biomass production and conidial viability were evaluated, and glucose was used as a carbon source to enhance conidial production. The optimized conditions that resulted in a maximum yield of conidia (1.78 x 107 conidia?mL-1), dried biomass (0.558?g) and CFUs (5.15 x 106 CFUs?mL-1) were a pH value of 4 and a photoperiod of 12?h. MenosAbstract: Optimization of the culture medium and the entire mass production process for various fungi in liquid medium has been studied. However, the technology is not well developed for Clonostachys rosea, a biocontrol agent against various plant pathogens due to its antagonistic capacity to act as a hyperparasite, compete for nutrients and space, and induce plant resistance to pathogens. In this study, we aimed to optimize the culture medium and to standardize parameters that may interfere with the production of C. rosea conidia in a liquid-state fermentation system. Culturing was performed in 250-mL Erlenmeyer flasks shaken for 7 days, followed by planned experimental methodology to reduce the number of analyses and consumable costs. Benchtop bioreactor tests with the optimized medium were performed. Glucose and sucrose were evaluated as carbon sources. Initially, the effects of temperature, pH, photoperiod, carbon:nitrogen ratio and water activity on inoculum production were evaluated, with the pH and photoperiod being factors that contributed to conidial production. Optimization of the fermentation conditions was performed using a central composite rotational design (CCD) with a wider range of pH values and photoperiods. The remaining variables were fixed according to the previous assay. Colony-forming unit (CFUs), biomass production and conidial viability were evaluated, and glucose was used as a carbon source to enhance conidial production. The optimized conditions th... Mostrar Tudo |
Palavras-Chave: |
Bioreactor; Liquid media; Produção massal. |
Thesagro: |
Fungo Para Controle Biológico. |
Thesaurus NAL: |
Biological control agents; Biomass production; Bioreactors; Clonostachys rosea. |
Categoria do assunto: |
H Saúde e Patologia |
Marc: |
LEADER 02628naa a2200313 a 4500 001 2093587 005 2018-07-26 008 2018 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.biocontrol.2017.11.014$2DOI 100 1 $aCARVALHO, A. L. de A. 245 $aOptimizing the mass production of Clonostachys rosea by liquid-state fermentation.$h[electronic resource] 260 $c2018 520 $aAbstract: Optimization of the culture medium and the entire mass production process for various fungi in liquid medium has been studied. However, the technology is not well developed for Clonostachys rosea, a biocontrol agent against various plant pathogens due to its antagonistic capacity to act as a hyperparasite, compete for nutrients and space, and induce plant resistance to pathogens. In this study, we aimed to optimize the culture medium and to standardize parameters that may interfere with the production of C. rosea conidia in a liquid-state fermentation system. Culturing was performed in 250-mL Erlenmeyer flasks shaken for 7 days, followed by planned experimental methodology to reduce the number of analyses and consumable costs. Benchtop bioreactor tests with the optimized medium were performed. Glucose and sucrose were evaluated as carbon sources. Initially, the effects of temperature, pH, photoperiod, carbon:nitrogen ratio and water activity on inoculum production were evaluated, with the pH and photoperiod being factors that contributed to conidial production. Optimization of the fermentation conditions was performed using a central composite rotational design (CCD) with a wider range of pH values and photoperiods. The remaining variables were fixed according to the previous assay. Colony-forming unit (CFUs), biomass production and conidial viability were evaluated, and glucose was used as a carbon source to enhance conidial production. The optimized conditions that resulted in a maximum yield of conidia (1.78 x 107 conidia?mL-1), dried biomass (0.558?g) and CFUs (5.15 x 106 CFUs?mL-1) were a pH value of 4 and a photoperiod of 12?h. 650 $aBiological control agents 650 $aBiomass production 650 $aBioreactors 650 $aClonostachys rosea 650 $aFungo Para Controle Biológico 653 $aBioreactor 653 $aLiquid media 653 $aProdução massal 700 1 $aREZENDE, L. C. de 700 1 $aCOSTA, L. B. 700 1 $aHALFELD-VIEIRA, B. de A. 700 1 $aPINTO, Z. V. 700 1 $aMORANDI, M. A. B. 700 1 $aMEDEIROS, F. H. V. 700 1 $aBETTIOL, W. 773 $tBiological Control$gv. 118, p. 16-25, 2018.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Meio Ambiente (CNPMA) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Expressão de busca inválida. Verifique!!! |
|
|