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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
19/02/2016 |
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Data da última atualização: |
19/02/2016 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SILVA, L. J.; DUARTE, A. W. F.; ROSA, L. H.; OLIVEIRA, V. M.; MELO, I. S. de. |
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Afiliação: |
L. J. SILVA, ESALQ/USP; A. W. F. DUARTE, USP; L. H. ROSA, UFMG; V. M. OLIVEIRA, CPQBA/Unicamp. |
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Título: |
Fosmid library: methodological challenges. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., Florianópolis. Anais... Florianópolis: Sociedade Brasileira de Microbiologia, 2015. Ref. 0676-1. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Since 1983, Brazil conducts researchs in the Antartic continent through the Brazilian Antartic Program. For many years, studies were focused only to the understanding of the environmental impacts of climate change resulting from human action and the system of micro.- and macrorganisms. The interest of the bioprospecting molecules for use in agriculture or health began only in the last decade in the same continent. Since about half of the known bioactive compounds are originating from bacteria and fungi, and a tiny portion of the microbiota is cultivable in the Laboratory. Recent advances mainly to independent cultivation techniques allow a greater understanding of microbial biodiversity as well as access to functionality of their metabolic pathways. In this context the heterologous expression from metagenomic clones from total environmental DNA (eDNA) it stands out as a promising tool to access the microbial functional part. The objective of this study was to evaluate the efficiency of the cloning procedure (metagenomic library of clones in fosmid vector - pCC2FOS) according to different methodologies of obtaining eDNA, selection of the gene inserts and conditions for the reaction for ligation to vector. The samples were collected at Admiralty Bay - Antarctic Peninsula, during last summer (Nov/Feb 2014-2015.) by the MycoAntar Project. The samples were submitted to extraction of eDNA high molecular weight such as to mechanical and chemical cell lysis procedures. A portion of the product extraction was partitioned by pulsed field gel electrophoresis from which they were recovered fragments containing approximately 40Kb and the other portion went directly to the subsequent procedure. The ligation reaction was made in two different temperatures 16-25°C. The result of the chemical cell lysis reaction was more efficient at obtaining size fragments of interest for the cloning procedure despite the quantitative reduction compared to extraction by mechanical lysis. The recovery procedure of the inserts gel using gelase enzyme resulted in drastic losses at the final concentration of eDNA. In relation to the difference in incubation temperature the binding reaction some differences can be observed. Thus 15,000 clones were obtained and are currently preserved at -80°C for next experiments as well as be submitted to the enzymatic screening functional biological activities of biotechnological interest. MenosAbstract: Since 1983, Brazil conducts researchs in the Antartic continent through the Brazilian Antartic Program. For many years, studies were focused only to the understanding of the environmental impacts of climate change resulting from human action and the system of micro.- and macrorganisms. The interest of the bioprospecting molecules for use in agriculture or health began only in the last decade in the same continent. Since about half of the known bioactive compounds are originating from bacteria and fungi, and a tiny portion of the microbiota is cultivable in the Laboratory. Recent advances mainly to independent cultivation techniques allow a greater understanding of microbial biodiversity as well as access to functionality of their metabolic pathways. In this context the heterologous expression from metagenomic clones from total environmental DNA (eDNA) it stands out as a promising tool to access the microbial functional part. The objective of this study was to evaluate the efficiency of the cloning procedure (metagenomic library of clones in fosmid vector - pCC2FOS) according to different methodologies of obtaining eDNA, selection of the gene inserts and conditions for the reaction for ligation to vector. The samples were collected at Admiralty Bay - Antarctic Peninsula, during last summer (Nov/Feb 2014-2015.) by the MycoAntar Project. The samples were submitted to extraction of eDNA high molecular weight such as to mechanical and chemical cell lysis procedures. A p... Mostrar Tudo |
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Palavras-Chave: |
Antarctic peninsula; Metagenomic library; Unculturable microrganisms. |
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Categoria do assunto: |
V Taxonomia de Organismos |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/139515/1/2015RA-008.pdf
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Marc: |
LEADER 03101nam a2200193 a 4500
001 2037800
005 2016-02-19
008 2015 bl uuuu u00u1 u #d
100 1 $aSILVA, L. J.
245 $aFosmid library$bmethodological challenges.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., Florianópolis. Anais... Florianópolis: Sociedade Brasileira de Microbiologia, 2015. Ref. 0676-1.$c2015
520 $aAbstract: Since 1983, Brazil conducts researchs in the Antartic continent through the Brazilian Antartic Program. For many years, studies were focused only to the understanding of the environmental impacts of climate change resulting from human action and the system of micro.- and macrorganisms. The interest of the bioprospecting molecules for use in agriculture or health began only in the last decade in the same continent. Since about half of the known bioactive compounds are originating from bacteria and fungi, and a tiny portion of the microbiota is cultivable in the Laboratory. Recent advances mainly to independent cultivation techniques allow a greater understanding of microbial biodiversity as well as access to functionality of their metabolic pathways. In this context the heterologous expression from metagenomic clones from total environmental DNA (eDNA) it stands out as a promising tool to access the microbial functional part. The objective of this study was to evaluate the efficiency of the cloning procedure (metagenomic library of clones in fosmid vector - pCC2FOS) according to different methodologies of obtaining eDNA, selection of the gene inserts and conditions for the reaction for ligation to vector. The samples were collected at Admiralty Bay - Antarctic Peninsula, during last summer (Nov/Feb 2014-2015.) by the MycoAntar Project. The samples were submitted to extraction of eDNA high molecular weight such as to mechanical and chemical cell lysis procedures. A portion of the product extraction was partitioned by pulsed field gel electrophoresis from which they were recovered fragments containing approximately 40Kb and the other portion went directly to the subsequent procedure. The ligation reaction was made in two different temperatures 16-25°C. The result of the chemical cell lysis reaction was more efficient at obtaining size fragments of interest for the cloning procedure despite the quantitative reduction compared to extraction by mechanical lysis. The recovery procedure of the inserts gel using gelase enzyme resulted in drastic losses at the final concentration of eDNA. In relation to the difference in incubation temperature the binding reaction some differences can be observed. Thus 15,000 clones were obtained and are currently preserved at -80°C for next experiments as well as be submitted to the enzymatic screening functional biological activities of biotechnological interest.
653 $aAntarctic peninsula
653 $aMetagenomic library
653 $aUnculturable microrganisms
700 1 $aDUARTE, A. W. F.
700 1 $aROSA, L. H.
700 1 $aOLIVEIRA, V. M.
700 1 $aMELO, I. S. de
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido; Embrapa Unidades Centrais. |
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Data corrente: |
31/03/2011 |
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Data da última atualização: |
25/07/2011 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
FERNANDES JUNIOR, P. I.; ALMEIDA, J. P. da S.; PASSOS, S. R.; OLIVEIRA, P. J. de; RUMJANEK, N. G.; XAVIER, G. R. |
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Afiliação: |
PAULO IVAN FERNANDES JUNIOR, CPATSA; Universidade Federal Rural do Rio de Janeiro (UFRRJ); UFRRJ, Instituto de Agronomia, Departamento de Solos; Universidade Federal Rural do Rio de Janeiro (UFRRJ); NORMA GOUVEA RUMJANEK, CNPAB; GUSTAVO RIBEIRO XAVIER, CN. |
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Título: |
Produção e comportamento reológico de exopolissacarídeos sintetizados por rizóbios isolados de guandu. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Pesquisa Agropecuaria Brasileira, Brasília, DF, v. 45, n. 12, p. 1465-1471, dez. 2010 |
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Idioma: |
Português |
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Conteúdo: |
O objetivo deste trabalho foi avaliar a produção e o comportamento reológico de exopolissacarídeos (EPS) produzidos por rizóbios isolados de nódulos de guandu [Cajanus cajan (L.) Millsp.], e a similaridade desses isolados, pela técnica de análise de restrição do DNA ribossomal amplificado. As bactérias foram cultivadas em meio YM líquido e os EPS foram obtidos por precipitação com etanol gelado a partir do caldo de cultivo. Em seguida, eles foram recuperados por centrifugação, secados a vácuo, pesados e ressuspensos em água para avaliações reológicas. Os três isolados avaliados apresentaram diferenças na produção e na eficiência relativa da produção de EPS, com destaque para o isolado 8.1c. Os EPS demonstraram comportamento não newtoniano e pseudoplástico, porém também apresentaram diferenças na viscosidade aparente em uma mesma taxa de cisalhamento. Na taxa de cisalhamento de 1 s-1, os três isolados foram diferentes, enquanto na taxa de cisalhamento de 40 s-1, os isolados 53.5 e 30.6a2 foram iguais e diferiram do isolado 8.1c. A similaridade dos isolados foi condizente com os resultados de reologia dos EPS, em que bactérias que sintetizaram EPS com menor viscosidade aparente apresentaram maior similaridade. |
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Palavras-Chave: |
ARDRA; Biological nitrogen fixation; Biopolímeros; Fixação biológica de nitrogênio; Inovação tecnológica; Reologia; Technological innovation. |
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Thesagro: |
Cajanus cajan. |
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Thesaurus NAL: |
Biopolymers; Rheology. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/37025/1/Paulo-Ivan.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/176568/1/Producao-e-comportamento-reologico-de-exopolissacrideos.pdf
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Marc: |
LEADER 02191naa a2200301 a 4500
001 1895186
005 2011-07-25
008 2010 bl uuuu u00u1 u #d
100 1 $aFERNANDES JUNIOR, P. I.
245 $aProdução e comportamento reológico de exopolissacarídeos sintetizados por rizóbios isolados de guandu.
260 $c2010
520 $aO objetivo deste trabalho foi avaliar a produção e o comportamento reológico de exopolissacarídeos (EPS) produzidos por rizóbios isolados de nódulos de guandu [Cajanus cajan (L.) Millsp.], e a similaridade desses isolados, pela técnica de análise de restrição do DNA ribossomal amplificado. As bactérias foram cultivadas em meio YM líquido e os EPS foram obtidos por precipitação com etanol gelado a partir do caldo de cultivo. Em seguida, eles foram recuperados por centrifugação, secados a vácuo, pesados e ressuspensos em água para avaliações reológicas. Os três isolados avaliados apresentaram diferenças na produção e na eficiência relativa da produção de EPS, com destaque para o isolado 8.1c. Os EPS demonstraram comportamento não newtoniano e pseudoplástico, porém também apresentaram diferenças na viscosidade aparente em uma mesma taxa de cisalhamento. Na taxa de cisalhamento de 1 s-1, os três isolados foram diferentes, enquanto na taxa de cisalhamento de 40 s-1, os isolados 53.5 e 30.6a2 foram iguais e diferiram do isolado 8.1c. A similaridade dos isolados foi condizente com os resultados de reologia dos EPS, em que bactérias que sintetizaram EPS com menor viscosidade aparente apresentaram maior similaridade.
650 $aBiopolymers
650 $aRheology
650 $aCajanus cajan
653 $aARDRA
653 $aBiological nitrogen fixation
653 $aBiopolímeros
653 $aFixação biológica de nitrogênio
653 $aInovação tecnológica
653 $aReologia
653 $aTechnological innovation
700 1 $aALMEIDA, J. P. da S.
700 1 $aPASSOS, S. R.
700 1 $aOLIVEIRA, P. J. de
700 1 $aRUMJANEK, N. G.
700 1 $aXAVIER, G. R.
773 $tPesquisa Agropecuaria Brasileira, Brasília, DF$gv. 45, n. 12, p. 1465-1471, dez. 2010
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