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Registro Completo |
Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
Data corrente: |
19/12/2023 |
Data da última atualização: |
29/12/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SANTOS, K. S.; COSTA, C.; BESSA, M. J.; TEIXEIRA, J. P.; SILVA, A. V. C. da; PADILHA, F. F.; DARIVA, C.; OLIVEIRA, M. B. P. P. |
Afiliação: |
KLEBSON SILVA SANTOS, ITP; CARLA COSTA, Portuguese National Institute of Health; MARIA JOÃO BESSA, Universidade do Porto; JOAO PAULO TEIXEIRA, UNIVERSIDADE DO PORTO; ANA VERUSKA CRUZ DA SILVA MUNIZ, CPATC; FRANCINE FERREIRA PADILHA, ITP; CLÁUDIO DARIVA, ITP; MARIA BEATRIZ PINTO PRIOR OLIVEIRA, UNIVERSIDADE DO PORTO. |
Título: |
Azadirachta indica A. Juss (neem) phenolic extract inhibits human B-lymphoblastoid cells growth via cell cycle arrest, apoptosis induction, and DNA damage. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Exploration of Foods and Foodomics, v. 1, p. 130-142, 2023. |
DOI: |
https://doi.org/10.37349/eff.2023.00011 |
Idioma: |
Inglês |
Conteúdo: |
Aim: As far as is known, the pharmaceutical effects of neem on human B-lymphoblastoid (TK6) cells have not been studied until now. Hence, the present study aimed to obtain neem phenolic extracts for inhibits the proliferation of TK6 cells and explore some possible underlying mechanisms involved in these effects. Methods: Hexane extract (HE) was obtained in the first step. After that, the residual hexane was removed
from the neem. The dried neem sample was used in a new extraction for obtaining the ethyl acetate extract (EAE). Total phenolic compounds (TPC) and total flavonoid contents (TFC) were determined by spectrophotometric methods. Lactate dehydrogenase (LDH) and 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) tests were used to evaluate the cytotoxicity in TK6 cells. The stop at G0/G1 cell cycle and inducing apoptosis in the TK6 cells were analyzed by flow cytometry. For deoxyribonucleic acid (DNA) damage evaluation, the alkaline comet test was used. Results: The higher TFC (65.50 mg/g of extract ± 1.17 mg/g of extract) and TPC (52.08 mg of extract ± 0.88 mg of extract) were obtained in EAE compared to HE that was obtained TFC of 14.61 mg/g of extract ± 0.60 mg/g of extract and TPC of 3.20 mg/g of extract ± 1.20 mg/g of extract. EAE was more significantly cytotoxic to TK6 cells than HE. The apoptosis induction was higher after exposure to 15.0 μg/mL of EAE (11.29%) in comparison to 15.0 μg/mL of HE (2.52%). The G0/G1 phase increased from 72% negative control (NC) to 83% after treatment with neem extracts (15 μg/mL). Neem extracts were also able to cause DNA strand breaks in TK6 cells. MenosAim: As far as is known, the pharmaceutical effects of neem on human B-lymphoblastoid (TK6) cells have not been studied until now. Hence, the present study aimed to obtain neem phenolic extracts for inhibits the proliferation of TK6 cells and explore some possible underlying mechanisms involved in these effects. Methods: Hexane extract (HE) was obtained in the first step. After that, the residual hexane was removed
from the neem. The dried neem sample was used in a new extraction for obtaining the ethyl acetate extract (EAE). Total phenolic compounds (TPC) and total flavonoid contents (TFC) were determined by spectrophotometric methods. Lactate dehydrogenase (LDH) and 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) tests were used to evaluate the cytotoxicity in TK6 cells. The stop at G0/G1 cell cycle and inducing apoptosis in the TK6 cells were analyzed by flow cytometry. For deoxyribonucleic acid (DNA) damage evaluation, the alkaline comet test was used. Results: The higher TFC (65.50 mg/g of extract ± 1.17 mg/g of extract) and TPC (52.08 mg of extract ± 0.88 mg of extract) were obtained in EAE compared to HE that was obtained TFC of 14.61 mg/g of extract ± 0.60 mg/g of extract and TPC of 3.20 mg/g of extract ± 1.20 mg/g of extract. EAE was more significantly cytotoxic to TK6 cells than HE. The apoptosis induction was higher after exposure to 15.0 μg/mL of EAE (11.29%) in comparison to 15.0 μg/mL of HE (2.52%). The G0/G1 phase increased from 72% negati... Mostrar Tudo |
Thesagro: |
Conservação; Nim; Planta Medicinal. |
Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
Marc: |
LEADER 02468naa a2200253 a 4500 001 2160010 005 2023-12-29 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.37349/eff.2023.00011$2DOI 100 1 $aSANTOS, K. S. 245 $aAzadirachta indica A. Juss (neem) phenolic extract inhibits human B-lymphoblastoid cells growth via cell cycle arrest, apoptosis induction, and DNA damage.$h[electronic resource] 260 $c2023 520 $aAim: As far as is known, the pharmaceutical effects of neem on human B-lymphoblastoid (TK6) cells have not been studied until now. Hence, the present study aimed to obtain neem phenolic extracts for inhibits the proliferation of TK6 cells and explore some possible underlying mechanisms involved in these effects. Methods: Hexane extract (HE) was obtained in the first step. After that, the residual hexane was removed from the neem. The dried neem sample was used in a new extraction for obtaining the ethyl acetate extract (EAE). Total phenolic compounds (TPC) and total flavonoid contents (TFC) were determined by spectrophotometric methods. Lactate dehydrogenase (LDH) and 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) tests were used to evaluate the cytotoxicity in TK6 cells. The stop at G0/G1 cell cycle and inducing apoptosis in the TK6 cells were analyzed by flow cytometry. For deoxyribonucleic acid (DNA) damage evaluation, the alkaline comet test was used. Results: The higher TFC (65.50 mg/g of extract ± 1.17 mg/g of extract) and TPC (52.08 mg of extract ± 0.88 mg of extract) were obtained in EAE compared to HE that was obtained TFC of 14.61 mg/g of extract ± 0.60 mg/g of extract and TPC of 3.20 mg/g of extract ± 1.20 mg/g of extract. EAE was more significantly cytotoxic to TK6 cells than HE. The apoptosis induction was higher after exposure to 15.0 μg/mL of EAE (11.29%) in comparison to 15.0 μg/mL of HE (2.52%). The G0/G1 phase increased from 72% negative control (NC) to 83% after treatment with neem extracts (15 μg/mL). Neem extracts were also able to cause DNA strand breaks in TK6 cells. 650 $aConservação 650 $aNim 650 $aPlanta Medicinal 700 1 $aCOSTA, C. 700 1 $aBESSA, M. J. 700 1 $aTEIXEIRA, J. P. 700 1 $aSILVA, A. V. C. da 700 1 $aPADILHA, F. F. 700 1 $aDARIVA, C. 700 1 $aOLIVEIRA, M. B. P. P. 773 $tExploration of Foods and Foodomics$gv. 1, p. 130-142, 2023.
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Embrapa Tabuleiros Costeiros (CPATC) |
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Registro Completo
Biblioteca(s): |
Embrapa Agrossilvipastoril; Embrapa Soja. |
Data corrente: |
26/01/2024 |
Data da última atualização: |
26/01/2024 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
RAMOS JUNIOR, E. U.; SPERA, S. T.; MAGALHÃES, C. A. de S.; VENDRUSCULO, L. G. |
Afiliação: |
EDISON ULISSES RAMOS JUNIOR, CNPSO; SILVIO TULIO SPERA, CPAMT; CIRO AUGUSTO DE SOUZA MAGALHAES, CNPMS; LAURIMAR GONCALVES VENDRUSCULO, CPAMT. |
Título: |
Evaluation of new phosphorus sources for the northern region of Mato Grosso, Brazil. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
In: WORLD SOYBEAN RESEARCH CONFERENCE, 11., 2023, Vienna. Soybean Research for Sustainable Development. Abstracts. Vienna: University of Natural Resources and Life Sciences, 2023. |
Volume: |
11 |
Páginas: |
p. 299. |
Idioma: |
Inglês Português |
Notas: |
WSRC |
Conteúdo: |
The objective of this study was to compare traditional sources of phosphorus with new technologies in order to offer to farmer’s operational advantages, making the operation less costly, besides to allowing the replacement of S jointly. |
Thesagro: |
Fósforo; Soja. |
Thesaurus NAL: |
Phosphorus; Soybeans. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 01001nam a2200229 a 4500 001 2161368 005 2024-01-26 008 2023 bl uuuu u00u1 u #d 100 1 $aRAMOS JUNIOR, E. U. 245 $aEvaluation of new phosphorus sources for the northern region of Mato Grosso, Brazil.$h[electronic resource] 260 $aIn: WORLD SOYBEAN RESEARCH CONFERENCE, 11., 2023, Vienna. Soybean Research for Sustainable Development. Abstracts. Vienna: University of Natural Resources and Life Sciences$c2023 300 $ap. 299. 11 490 $v11 500 $aWSRC 520 $aThe objective of this study was to compare traditional sources of phosphorus with new technologies in order to offer to farmer’s operational advantages, making the operation less costly, besides to allowing the replacement of S jointly. 650 $aPhosphorus 650 $aSoybeans 650 $aFósforo 650 $aSoja 700 1 $aSPERA, S. T. 700 1 $aMAGALHÃES, C. A. de S. 700 1 $aVENDRUSCULO, L. G.
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