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| 10. |  | CERBITO, W. A.; VILLAR, E. C.; SATO, K.; QUERO JUNIOR, F.; BALAGAPO JUNIOR, C. R.; FAYLON, P. S. Goat-crop-fodder integrated farming system at village level. Indian Journal of Animal Science, v.65, n.2, p.218-221, 1995.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 11. | | CARREIRA, L. M. M.; LUCAS, F. C. A.; MAUES, M. M.; PEREIRA, A. S. de S.; SATO, K. S. S. Caracterização de tipos polínicos transportados por abelhas em sistemas agroflorestais com dendê (Elaeis guineensis Jacq. - Arecaceae). In: CONGRESSO BRASILEIRO DE SISTEMAS AGROFLORESTAIS, 8., 2011, Belém, PA. Anais... Belém, PA: SBSAF: Embrapa Amazônia Oriental: UFRA: CEPLAC: EMATER: ICRAF, 2011. 1 CD- ROM. Editores técnicos: Roberto Porro, Milton Kanashiro, Maria do Socorro Gonçalves Ferreira, Leila Sobral Sampaio e Gladys Ferreira de Sousa.| Biblioteca(s): Embrapa Amazônia Oriental. |
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Registro Completo
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Biblioteca(s): |
Embrapa Uva e Vinho. |
|
Data corrente: |
18/10/2016 |
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Data da última atualização: |
06/05/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
FRAGA, H. P. de F.; VIEIRA, L. do N.; PUTTKAMMER, C. C.; SANTOS, H. P. dos; GARIGHAN, J. de A.; GUERRA, M. P. |
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Afiliação: |
Hugo pacheco de Freitas Fraga, Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Florianópolis, SC, 88034-001, Brazil; Leila do Nascimento Vieira, Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Florianópolis, SC, 88034-001, Brazil; Catarina Corrêa Puttkammer, Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Florianópolis, SC, 88034-001, Brazil; HENRIQUE PESSOA DOS SANTOS, CNPUV; Julio de Andrade Garighan, Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Florianópolis, SC, 88034-001, Brazil; Miguel PEdro Guerra, Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Florianópolis, SC, 88034-001, Brazil. |
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Título: |
Glutathione and abscisic acid supplementation influences somatic embryo maturation and hormone endogenous levels during somatic embryogenesis in Podocarpus lambertii Klotzsch ex Endl. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Plant Science, n. 235, p. 98-106, 2016. |
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DOI: |
http://dx.doi.org/10.1016/j.plantsci.2016.09.012 |
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Idioma: |
Inglês |
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Conteúdo: |
Here we propose a protocol for embryogenic cultures induction, proliferation and maturation for the Brazilian conifer Podocarpus lambertii, and investigated the effect of abscisic acid (ABA) and glutathione (GSH) supplementation on the maturation phase. ABA, zeatin (Z) and salicylic acid (SA) endogenous levels were quantified. Number of somatic embryos obtained in ABA-supplemented treatment was signifi- cant higher than in ABA-free treatment, showing the relevance of ABA supplementation during somatic embryos maturation. Histological analysis showed the stereotyped sequence of developmental stages in conifer somatic embryos, reaching the late torpedo-staged embryo. GSH supplementation in maturation culture medium improved the somatic embryos number and morphological features. GSH 0 mM and GSH 0.1 mM treatments correlated with a decreased ABA endogenous level during maturation, while GSH 0.5 mM treatment showed constantlevels. Alltreatments resulted in decreased Z endogenous levels, supporting the concept that cytokinins are important during the initial cell division but not for the later stages of embryo development. The lowest SA levels found in GSH 0.5 mM treatment were coincident with early embryonic development, and this treatment resulted in the highest development of somatic embryos. Thus, a correlation between lower SA levels and improved somatic embryo formation can be hypothesized |
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Palavras-Chave: |
Brazilian conifer; Embryonic development; Redox metabolism. |
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Thesaurus NAL: |
In vitro culture; Micropropagation. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/148901/1/GSHPodHugo-PlSci16.pdf
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Marc: |
LEADER 02293naa a2200253 a 4500
001 2054895
005 2019-05-06
008 2016 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1016/j.plantsci.2016.09.012$2DOI
100 1 $aFRAGA, H. P. de F.
245 $aGlutathione and abscisic acid supplementation influences somatic embryo maturation and hormone endogenous levels during somatic embryogenesis in Podocarpus lambertii Klotzsch ex Endl.$h[electronic resource]
260 $c2016
520 $aHere we propose a protocol for embryogenic cultures induction, proliferation and maturation for the Brazilian conifer Podocarpus lambertii, and investigated the effect of abscisic acid (ABA) and glutathione (GSH) supplementation on the maturation phase. ABA, zeatin (Z) and salicylic acid (SA) endogenous levels were quantified. Number of somatic embryos obtained in ABA-supplemented treatment was signifi- cant higher than in ABA-free treatment, showing the relevance of ABA supplementation during somatic embryos maturation. Histological analysis showed the stereotyped sequence of developmental stages in conifer somatic embryos, reaching the late torpedo-staged embryo. GSH supplementation in maturation culture medium improved the somatic embryos number and morphological features. GSH 0 mM and GSH 0.1 mM treatments correlated with a decreased ABA endogenous level during maturation, while GSH 0.5 mM treatment showed constantlevels. Alltreatments resulted in decreased Z endogenous levels, supporting the concept that cytokinins are important during the initial cell division but not for the later stages of embryo development. The lowest SA levels found in GSH 0.5 mM treatment were coincident with early embryonic development, and this treatment resulted in the highest development of somatic embryos. Thus, a correlation between lower SA levels and improved somatic embryo formation can be hypothesized
650 $aIn vitro culture
650 $aMicropropagation
653 $aBrazilian conifer
653 $aEmbryonic development
653 $aRedox metabolism
700 1 $aVIEIRA, L. do N.
700 1 $aPUTTKAMMER, C. C.
700 1 $aSANTOS, H. P. dos
700 1 $aGARIGHAN, J. de A.
700 1 $aGUERRA, M. P.
773 $tPlant Science$gn. 235, p. 98-106, 2016.
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