02293naa a2200253 a 450000100080000000500110000800800410001902400580006010000230011824502110014126000090035252014170036165000210177865000210179965300220182065300260184265300210186870000210188970000220191070000220193270000230195470000180197777300440199520548952019-05-06       2016    bl uuuu     u00u1 u    #d7 ahttp://dx.doi.org/10.1016/j.plantsci.2016.09.0122DOI1 aFRAGA, H. P. de F.  aGlutathione and abscisic acid supplementation influences somatic embryo maturation and hormone endogenous levels during somatic embryogenesis in Podocarpus lambertii Klotzsch ex Endl.h[electronic resource]  c2016  aHere we propose a protocol for embryogenic cultures induction, proliferation and maturation for the Brazilian conifer Podocarpus lambertii, and investigated the effect of abscisic acid (ABA) and glutathione (GSH) supplementation on the maturation phase. ABA, zeatin (Z) and salicylic acid (SA) endogenous levels were quantified. Number of somatic embryos obtained in ABA-supplemented treatment was signifi- cant higher than in ABA-free treatment, showing the relevance of ABA supplementation during somatic embryos maturation. Histological analysis showed the stereotyped sequence of developmental stages in conifer somatic embryos, reaching the late torpedo-staged embryo. GSH supplementation in maturation culture medium improved the somatic embryos number and morphological features. GSH 0 mM and GSH 0.1 mM treatments correlated with a decreased ABA endogenous level during maturation, while GSH 0.5 mM treatment showed constantlevels. Alltreatments resulted in decreased Z endogenous levels, supporting the concept that cytokinins are important during the initial cell division but not for the later stages of embryo development. The lowest SA levels found in GSH 0.5 mM treatment were coincident with early embryonic development, and this treatment resulted in the highest development of somatic embryos. Thus, a correlation between lower SA levels and improved somatic embryo formation can be hypothesized  aIn vitro culture  aMicropropagation  aBrazilian conifer  aEmbryonic development  aRedox metabolism1 aVIEIRA, L. do N.1 aPUTTKAMMER, C. C.1 aSANTOS, H. P. dos1 aGARIGHAN, J. de A.1 aGUERRA, M. P.  tPlant Sciencegn. 235, p. 98-106, 2016.