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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
21/10/2019 |
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Data da última atualização: |
21/10/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, K. C. de; GUIMARÃES, P. de S.; BAZIOLI, J. M.; MARTINATI, J. C.; SANTOS, M. M. dos; PADILHA, L.; GUERREIRO-FILHO, O.; MALUF, M. P. |
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Afiliação: |
Kenia Carvalho de Oliveira, Coffee Center Agronomic Institute - IAC; Paula de Souza Guimarães, Coffee Center Agronomic Institute - IAC; Jaqueline Moraes Bazioli, Coffee Center Agronomic Institute - IAC; Juliana Camargo Martinati, Coffee Center Agronomic Institute - IAC; Mariana Martinis dos Santos, Coffee Center Agronomic Institute - IAC; LILIAN PADILHA, CNPCa; Oliveiro Guerreiro-Filho, Coffee Center Agronomic Institute - IAC; MIRIAN PEREZ MALUF, CNPCa. |
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Título: |
Effects of somatic embryogenesis on gene expression of cloned coffee heterozygous hybrids. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Acta Physiologiae Plantarum, v. 41, n. 7, July 2019. |
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Idioma: |
Inglês |
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Conteúdo: |
Cloning of superior coffee plants by somatic embryogenesis can assist breeding programs on reducing the cost and time for launch of new cultivars. This study aimed to evaluate the efficiency of this methodology for cloning coffee trees with high heterozygosity, and to gather evidence that clonal progenies are faithful copies of mother plants. Selected plants IAC1 and IAC 2 from Coffea arabica breeding populations, resistant to leaf rust and leaf miner, respectively, were cloned via indirect somatic embryogenesis. Expression of selected genes involved in biological processes potentially affected by in vitro cultivation was evaluated by quantitative analysis. Genes encoding proteins associated with maintenance of DNA integrity and control of cell cycle presented predictable expression patterns along the clonal multiplication process. There were differences in the expression pattern of genes linked to in vitro cultivation-related stress, which were observed comparing either IAC 1 and IAC2 genotypes or clones and their corresponding mother plant. Those analyses suggest that the somatic embryogenesis does not lead to major genomic instability and clones are identical copies of mother plants, even with detected differences in the expression of genes that influence the response of in vitro cultivation. |
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Palavras-Chave: |
Differential expression; In vitro cultivation. |
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Thesagro: |
Coffea Arábica. |
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Thesaurus Nal: |
Somaclonal variation. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/203223/1/Effects-of-somatic-embryogenesis-on-gene.pdf
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Marc: |
LEADER 02084naa a2200253 a 4500
001 2113261
005 2019-10-21
008 2019 bl uuuu u00u1 u #d
100 1 $aOLIVEIRA, K. C. de
245 $aEffects of somatic embryogenesis on gene expression of cloned coffee heterozygous hybrids.$h[electronic resource]
260 $c2019
520 $aCloning of superior coffee plants by somatic embryogenesis can assist breeding programs on reducing the cost and time for launch of new cultivars. This study aimed to evaluate the efficiency of this methodology for cloning coffee trees with high heterozygosity, and to gather evidence that clonal progenies are faithful copies of mother plants. Selected plants IAC1 and IAC 2 from Coffea arabica breeding populations, resistant to leaf rust and leaf miner, respectively, were cloned via indirect somatic embryogenesis. Expression of selected genes involved in biological processes potentially affected by in vitro cultivation was evaluated by quantitative analysis. Genes encoding proteins associated with maintenance of DNA integrity and control of cell cycle presented predictable expression patterns along the clonal multiplication process. There were differences in the expression pattern of genes linked to in vitro cultivation-related stress, which were observed comparing either IAC 1 and IAC2 genotypes or clones and their corresponding mother plant. Those analyses suggest that the somatic embryogenesis does not lead to major genomic instability and clones are identical copies of mother plants, even with detected differences in the expression of genes that influence the response of in vitro cultivation.
650 $aSomaclonal variation
650 $aCoffea Arábica
653 $aDifferential expression
653 $aIn vitro cultivation
700 1 $aGUIMARÃES, P. de S.
700 1 $aBAZIOLI, J. M.
700 1 $aMARTINATI, J. C.
700 1 $aSANTOS, M. M. dos
700 1 $aPADILHA, L.
700 1 $aGUERREIRO-FILHO, O.
700 1 $aMALUF, M. P.
773 $tActa Physiologiae Plantarum$gv. 41, n. 7, July 2019.
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Registro original: |
Embrapa Café (CNPCa) |
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Registro Completo
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
25/06/2003 |
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Data da última atualização: |
28/09/2010 |
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Autoria: |
SERENO, J. R. B.; DELGADO, J. V.; SERENO, F. T. P. S.; BARBA, C.; CABELLO, A. |
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Afiliação: |
JOSE ROBSON BEZERRA SERENO, CPAP. |
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Título: |
Bases de la caracterización del cerdo ibérico por la aptitud sexual del macho como clave para su conservación. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
In: Porci. Aula Veterinária - Monografias de actualidad, Madrid, n.73, p.11-19, ene., 2003. |
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Idioma: |
Espanhol |
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Conteúdo: |
En los últimos años las distintas variedades que configuran la raza porcina de cerdo Ibérico han sido caracterizadas desde el punto de vista de la conservación en los ámbitos morfológico, reproductivo y genético quedando pendiente la caracterización de la aptitud sexual del macho, siendo ésta el objetivo central de esta monografia. En el presente capítulo hacemos referencia al interés del conocimiento del comportamiento sexual del cerdo Ibérico abordando las bases del entrenamiento de los sementales frente al maniquí, el proceso de extracción de semen, la frecuencia de extracción seminal y las propias particularidades de esta área del comportamiento. Asimismo también se pone de manifiesto la importancia y aplicación de este comportamiento relacionado con otros factores importantes dentro de los programas de reproducción asistida como son la detección del celo, el momento de la ovolación y el momento óptimo de la inseminación artificial. |
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Palavras-Chave: |
Conservation; Iberian pib; Porco ibérico. |
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Thesagro: |
Comportamento Sexual; Conservação; Inseminação Artificial; Reprodução. |
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Thesaurus NAL: |
reproduction. |
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Categoria do assunto: |
-- |
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URL: |
https://www.aulaveterinaria.com
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Marc: |
LEADER 01780naa a2200265 a 4500
001 1790661
005 2010-09-28
008 2003 bl --- 0-- u #d
100 1 $aSERENO, J. R. B.
245 $aBases de la caracterización del cerdo ibérico por la aptitud sexual del macho como clave para su conservación.
260 $c2003
520 $aEn los últimos años las distintas variedades que configuran la raza porcina de cerdo Ibérico han sido caracterizadas desde el punto de vista de la conservación en los ámbitos morfológico, reproductivo y genético quedando pendiente la caracterización de la aptitud sexual del macho, siendo ésta el objetivo central de esta monografia. En el presente capítulo hacemos referencia al interés del conocimiento del comportamiento sexual del cerdo Ibérico abordando las bases del entrenamiento de los sementales frente al maniquí, el proceso de extracción de semen, la frecuencia de extracción seminal y las propias particularidades de esta área del comportamiento. Asimismo también se pone de manifiesto la importancia y aplicación de este comportamiento relacionado con otros factores importantes dentro de los programas de reproducción asistida como son la detección del celo, el momento de la ovolación y el momento óptimo de la inseminación artificial.
650 $areproduction
650 $aComportamento Sexual
650 $aConservação
650 $aInseminação Artificial
650 $aReprodução
653 $aConservation
653 $aIberian pib
653 $aPorco ibérico
700 1 $aDELGADO, J. V.
700 1 $aSERENO, F. T. P. S.
700 1 $aBARBA, C.
700 1 $aCABELLO, A.
773 $tIn: Porci. Aula Veterinária - Monografias de actualidad, Madrid$gn.73, p.11-19, ene., 2003.
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