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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
21/01/2019 |
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Data da última atualização: |
12/02/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ALVES-FREITAS, D. M. T.; PINHEIRO-LIMA, B.; FARIA, J. C.; LACORTE, C.; RIBEIRO, S. G.; MELO, F. L. |
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Afiliação: |
DIONE M. T. ALVES-FREITAS; BRUNA PINHEIRO-LIMA; JOSIAS CORREA DE FARIA, CNPAF; CRISTIANO CASTRO LACORTE, Cenargen; SIMONE DA GRACA RIBEIRO, Cenargen; FERNANDO L. MELO, UNB. |
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Título: |
Double-stranded RNA high-throughput sequencing reveals a new Cytorhabdovirus in a bean golden mosaic virus-resistant common bean transgenic line. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Viruses, v. 11, n. 1, Jan. 2019. |
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ISSN: |
1999-4915 |
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DOI: |
10.3390/v11010090 |
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Idioma: |
Inglês |
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Conteúdo: |
Using double-strand RNA (dsRNA) high-throughput sequencing, we identified five RNA viruses in a bean golden mosaic virus (BGMV)-resistant common bean transgenic line with symptoms of viral infection. Four of the identified viruses had already been described as infecting common bean (cowpea mild mottle virus, bean rugose mosaic virus, Phaseolus vulgaris alphaendornavirus 1, and Phaseolus vulgaris alphaendornavirus 2) and one is a putative new plant rhabdovirus (genus Cytorhabdovirus), tentatively named bean-associated cytorhabdovirus (BaCV). The BaCV genome presented all five open reading frames (ORFs) found in most rhabdoviruses: nucleoprotein (N) (ORF1) (451 amino acids, aa), phosphoprotein (P) (ORF2) (445 aa), matrix (M) (ORF4) (287 aa), glycoprotein (G) (ORF5) (520 aa), and an RNA-dependent RNA polymerase (L) (ORF6) (114 aa), as well as a putative movement protein (P3) (ORF3) (189 aa) and the hypothetical small protein P4. The predicted BaCV proteins were compared to homologous proteins from the closest cytorhabdoviruses, and a low level of sequence identity (15?39%) was observed. The phylogenetic analysis shows that BaCV clustered with yerba mate chlorosis-associated virus (YmCaV) and rice stripe mosaic virus (RSMV). Overall, our results provide strong evidence that BaCV is indeed a new virus species in the genus Cytorhabdovirus (family Rhabdoviridae), the first rhabdovirus to be identified infecting common bean. |
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Palavras-Chave: |
Rhabdovirus. |
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Thesagro: |
Feijão; Mosaico Dourado; Organismo Transgênico; Phaseolus Vulgaris; Vírus. |
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Thesaurus Nal: |
Bean golden mosaic virus; Beans; Cytorhabdovirus; DsRNA viruses; Transgenic plants. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/191029/1/CNPAF-2019-viruses.pdf
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Marc: |
LEADER 02429naa a2200337 a 4500
001 2104582
005 2020-02-12
008 2019 bl uuuu u00u1 u #d
022 $a1999-4915
024 7 $a10.3390/v11010090$2DOI
100 1 $aALVES-FREITAS, D. M. T.
245 $aDouble-stranded RNA high-throughput sequencing reveals a new Cytorhabdovirus in a bean golden mosaic virus-resistant common bean transgenic line.$h[electronic resource]
260 $c2019
520 $aUsing double-strand RNA (dsRNA) high-throughput sequencing, we identified five RNA viruses in a bean golden mosaic virus (BGMV)-resistant common bean transgenic line with symptoms of viral infection. Four of the identified viruses had already been described as infecting common bean (cowpea mild mottle virus, bean rugose mosaic virus, Phaseolus vulgaris alphaendornavirus 1, and Phaseolus vulgaris alphaendornavirus 2) and one is a putative new plant rhabdovirus (genus Cytorhabdovirus), tentatively named bean-associated cytorhabdovirus (BaCV). The BaCV genome presented all five open reading frames (ORFs) found in most rhabdoviruses: nucleoprotein (N) (ORF1) (451 amino acids, aa), phosphoprotein (P) (ORF2) (445 aa), matrix (M) (ORF4) (287 aa), glycoprotein (G) (ORF5) (520 aa), and an RNA-dependent RNA polymerase (L) (ORF6) (114 aa), as well as a putative movement protein (P3) (ORF3) (189 aa) and the hypothetical small protein P4. The predicted BaCV proteins were compared to homologous proteins from the closest cytorhabdoviruses, and a low level of sequence identity (15?39%) was observed. The phylogenetic analysis shows that BaCV clustered with yerba mate chlorosis-associated virus (YmCaV) and rice stripe mosaic virus (RSMV). Overall, our results provide strong evidence that BaCV is indeed a new virus species in the genus Cytorhabdovirus (family Rhabdoviridae), the first rhabdovirus to be identified infecting common bean.
650 $aBean golden mosaic virus
650 $aBeans
650 $aCytorhabdovirus
650 $aDsRNA viruses
650 $aTransgenic plants
650 $aFeijão
650 $aMosaico Dourado
650 $aOrganismo Transgênico
650 $aPhaseolus Vulgaris
650 $aVírus
653 $aRhabdovirus
700 1 $aPINHEIRO-LIMA, B.
700 1 $aFARIA, J. C.
700 1 $aLACORTE, C.
700 1 $aRIBEIRO, S. G.
700 1 $aMELO, F. L.
773 $tViruses$gv. 11, n. 1, Jan. 2019.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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| 13. |  | RIBEIRO, J. L.; RIBEIRO, V. Q. Avaliação agronômica de genótipos de girassol no município de Mata Roma, MA: ano agrícola de 2007/2008. In: CONGRESSO BRASILEIRO DE MAMONA, 4.; SIMPÓSIO INTERNACIONAL DE OLEAGINOSAS ENERGÉTICAS, 1., 2010, João Pessoa. Inclusão social e energia: anais. Campina Grande: Embrapa Algodão, 2010. p. 1542-1546 1 CD-ROM. Trabalho publicado também no Livro de resumos do CONGRESSO BRASILEIRO DE MAMONA, 4.; SIMPÓSIO INTERNACIONAL DE OLEAGINOSAS ENERGÉTICAS, 1., 2010, João Pessoa.| Tipo: Artigo em Anais de Congresso |
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