02429naa a2200337 a 450000100080000000500110000800800410001902200140006002400270007410000280010124501730012926000090030252014450031165000290175665000100178565000200179565000180181565000220183365000120185565000200186765000270188765000230191465000110193765300160194870000220196470000170198670000160200370000190201970000160203877300370205421045822020-02-12       2019    bl uuuu     u00u1 u    #d  a1999-49157 a10.3390/v110100902DOI1 aALVES-FREITAS, D. M. T.  aDouble-stranded RNA high-throughput sequencing reveals a new Cytorhabdovirus in a bean golden mosaic virus-resistant common bean transgenic line.h[electronic resource]  c2019  aUsing double-strand RNA (dsRNA) high-throughput sequencing, we identified five RNA viruses in a bean golden mosaic virus (BGMV)-resistant common bean transgenic line with symptoms of viral infection. Four of the identified viruses had already been described as infecting common bean (cowpea mild mottle virus, bean rugose mosaic virus, Phaseolus vulgaris alphaendornavirus 1, and Phaseolus vulgaris alphaendornavirus 2) and one is a putative new plant rhabdovirus (genus Cytorhabdovirus), tentatively named bean-associated cytorhabdovirus (BaCV). The BaCV genome presented all five open reading frames (ORFs) found in most rhabdoviruses: nucleoprotein (N) (ORF1) (451 amino acids, aa), phosphoprotein (P) (ORF2) (445 aa), matrix (M) (ORF4) (287 aa), glycoprotein (G) (ORF5) (520 aa), and an RNA-dependent RNA polymerase (L) (ORF6) (114 aa), as well as a putative movement protein (P3) (ORF3) (189 aa) and the hypothetical small protein P4. The predicted BaCV proteins were compared to homologous proteins from the closest cytorhabdoviruses, and a low level of sequence identity (15?39%) was observed. The phylogenetic analysis shows that BaCV clustered with yerba mate chlorosis-associated virus (YmCaV) and rice stripe mosaic virus (RSMV). Overall, our results provide strong evidence that BaCV is indeed a new virus species in the genus Cytorhabdovirus (family Rhabdoviridae), the first rhabdovirus to be identified infecting common bean.  aBean golden mosaic virus  aBeans  aCytorhabdovirus  aDsRNA viruses  aTransgenic plants  aFeijão  aMosaico Dourado  aOrganismo Transgênico  aPhaseolus Vulgaris  aVírus  aRhabdovirus1 aPINHEIRO-LIMA, B.1 aFARIA, J. C.1 aLACORTE, C.1 aRIBEIRO, S. G.1 aMELO, F. L.  tVirusesgv. 11, n. 1, Jan. 2019.