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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
12/12/2016 |
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Data da última atualização: |
16/11/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RAMOS-GONZÁLEZ, P. L.; CHABI-JESUS, C.; GUERRA-PERAZA, O.; BRETON, M. C.; ARENA, G. D.; NUNES, M. A.; KITAJIMA, E. W.; MACHADO, M. A.; ASTUA, J. de F. |
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Afiliação: |
PEDRO LUIS RAMOS-GONZÁLEZ, IAC; CAMILA CHABI-JESUS; ORLENE GUERRA-PERAZA, Instituto Biológico; MICHÈLE CLAIRE BRETON, IAC; GABRIELLA DIAS ARENA, IAC; MARIA ANDREIA NUNES, IAC; ELLIOT WATANABE KITAJIMA, ESALQ; MARCOS ANTONIO MACHADO, IAC; JULIANA DE FREITAS ASTUA, CNPMF. |
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Título: |
Phylogenetic and Molecular Variability Studies Reveal a New Genetic Clade of Citrus leprosis virus C. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Viruses, v.8, n.153, 2016. |
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ISSN: |
1999-4915 |
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DOI: |
10.3390/v8060153 |
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Idioma: |
Português |
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Conteúdo: |
Citrus leprosis virus C (CiLV-C) causes a severe disease affecting citrus orchards in the Western hemisphere. This study reveals the molecular variability of the virus by analyzing four genomic regions (p29, p15, MP and RNA2-intergenic region) distributed over its two RNAs. Nucleotide diversity () values were relatively low but statistically different over the analyzed genes and subpopulations, indicating their distinct evolutionary history. Values of p29 and MP were higher than those of p15 and RNA2?IR, whereas MP was increased due to novel discovered isolates phylogenetically clustered in a divergent clade that we called SJP. Isolate BR_SP_SJP_01 RNA1 and RNA2 sequences, clade SJP, showed an identity of 85.6% and 88.4%, respectively, with those corresponding to CiLV-C, the type member of the genus Cilevirus, and its RNA2 51-proximal region was revealed as a minor donor in a putative inter-clade recombination event. In addition to citrus, BR_SP_SJP_01 naturally infects the weed Commelina benghalensis and is efficiently transmitted by Brevipalpus yothersi mites. Our data demonstrated that negative selection was the major force operating in the evaluated viral coding regions and defined amino acids putatively relevant for the biological function of cilevirus proteins. This work provides molecular tools and sets up a framework for further epidemiological studies |
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Palavras-Chave: |
Plant diseases. |
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Thesagro: |
Doença de planta; Fruta cítrica. |
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Thesaurus Nal: |
Citrus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02197naa a2200289 a 4500
001 2058412
005 2017-11-16
008 2016 bl uuuu u00u1 u #d
022 $a1999-4915
024 7 $a10.3390/v8060153$2DOI
100 1 $aRAMOS-GONZÁLEZ, P. L.
245 $aPhylogenetic and Molecular Variability Studies Reveal a New Genetic Clade of Citrus leprosis virus C.$h[electronic resource]
260 $c2016
520 $aCitrus leprosis virus C (CiLV-C) causes a severe disease affecting citrus orchards in the Western hemisphere. This study reveals the molecular variability of the virus by analyzing four genomic regions (p29, p15, MP and RNA2-intergenic region) distributed over its two RNAs. Nucleotide diversity () values were relatively low but statistically different over the analyzed genes and subpopulations, indicating their distinct evolutionary history. Values of p29 and MP were higher than those of p15 and RNA2?IR, whereas MP was increased due to novel discovered isolates phylogenetically clustered in a divergent clade that we called SJP. Isolate BR_SP_SJP_01 RNA1 and RNA2 sequences, clade SJP, showed an identity of 85.6% and 88.4%, respectively, with those corresponding to CiLV-C, the type member of the genus Cilevirus, and its RNA2 51-proximal region was revealed as a minor donor in a putative inter-clade recombination event. In addition to citrus, BR_SP_SJP_01 naturally infects the weed Commelina benghalensis and is efficiently transmitted by Brevipalpus yothersi mites. Our data demonstrated that negative selection was the major force operating in the evaluated viral coding regions and defined amino acids putatively relevant for the biological function of cilevirus proteins. This work provides molecular tools and sets up a framework for further epidemiological studies
650 $aCitrus
650 $aDoença de planta
650 $aFruta cítrica
653 $aPlant diseases
700 1 $aCHABI-JESUS, C.
700 1 $aGUERRA-PERAZA, O.
700 1 $aBRETON, M. C.
700 1 $aARENA, G. D.
700 1 $aNUNES, M. A.
700 1 $aKITAJIMA, E. W.
700 1 $aMACHADO, M. A.
700 1 $aASTUA, J. de F.
773 $tViruses$gv.8, n.153, 2016.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 2 | |
| 1. |  | LEÃO, E.; VARELLA, B. B.; GALASSO, T.; NÁVIA, D.; POLEZ, V. L. P. Desenvolvimento de marcadores moleculares para a identificação de ácaros do gênero Brevipalpus. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 13., 2008, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. Resumo 170. p. 227.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 2. | | VARELLA, B. B.; LEÃO, E.; GALASSO, T.; NÁVIA, D.; POLEZ, V. L. P. Comparação de métodos de preservação de ácaros Brevipalpus phoenecis. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 13., 2008, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. Resumo 168. p. 224.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 2 | |
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