02197naa a2200289 a 450000100080000000500110000800800410001902200140006002400260007410000270010024501290012726000090025652013870026565000110165265000220166365000190168565300190170470000200172370000220174370000180176570000170178370000170180070000200181770000190183770000200185677300310187620584122017-11-16       2016    bl uuuu     u00u1 u    #d  a1999-49157 a10.3390/v80601532DOI1 aRAMOS-GONZÁLEZ, P. L.  aPhylogenetic and Molecular Variability Studies Reveal a New Genetic Clade of Citrus leprosis virus C.h[electronic resource]  c2016  aCitrus leprosis virus C (CiLV-C) causes a severe disease affecting citrus orchards in the Western hemisphere. This study reveals the molecular variability of the virus by analyzing four genomic regions (p29, p15, MP and RNA2-intergenic region) distributed over its two RNAs. Nucleotide diversity () values were relatively low but statistically different over the analyzed genes and subpopulations, indicating their distinct evolutionary history. Values of p29 and MP were higher than those of p15 and RNA2?IR, whereas MP was increased due to novel discovered isolates phylogenetically clustered in a divergent clade that we called SJP. Isolate BR_SP_SJP_01 RNA1 and RNA2 sequences, clade SJP, showed an identity of 85.6% and 88.4%, respectively, with those corresponding to CiLV-C, the type member of the genus Cilevirus, and its RNA2 51-proximal region was revealed as a minor donor in a putative inter-clade recombination event. In addition to citrus, BR_SP_SJP_01 naturally infects the weed Commelina benghalensis and is efficiently transmitted by Brevipalpus yothersi mites. Our data demonstrated that negative selection was the major force operating in the evaluated viral coding regions and defined amino acids putatively relevant for the biological function of cilevirus proteins. This work provides molecular tools and sets up a framework for further epidemiological studies  aCitrus  aDoença de planta  aFruta cítrica  aPlant diseases1 aCHABI-JESUS, C.1 aGUERRA-PERAZA, O.1 aBRETON, M. C.1 aARENA, G. D.1 aNUNES, M. A.1 aKITAJIMA, E. W.1 aMACHADO, M. A.1 aASTUA, J. de F.  tVirusesgv.8, n.153, 2016.