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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
20/11/2019 |
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Data da última atualização: |
24/04/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PAIVA, B. A. R. de; WENDLAND, A.; TEIXEIRA, N. C.; FERREIRA, M. A. S. V. |
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Afiliação: |
BRUNA ALICIA RAFAEL DE PAIVA, UNB; ADRIANE WENDLAND FERREIRA, CNPAF; NARA CRISTINA TEIXEIRA, bolsista CNPAF; MARISA A. S. V. FERREIRA, UNB. |
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Título: |
Rapid detection of Xanthomonas citri pv. fuscans and Xanthomonas phaseoli pv. phaseoli in common bean by loop-mediated isothermal amplification. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Plant Disease, v. 104, n. 1, p. 198-203, Jan. 2020. |
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ISSN: |
0191-2917 |
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DOI: |
10.1094/PDIS-02-19-0325-RE |
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Idioma: |
Inglês |
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Conteúdo: |
A single loop-mediated isothermal amplification (LAMP) assay was developed for specific detection of both pathogens that cause bacterial blight in common bean, Xanthomonas phaseoli pv. phaseoli (Xpp) and Xanthomonas citri pv. fuscans (Xcf). The objective was to provide a simple, easy-to-use, specific, and sensitive method to investigate the presence of one or both pathogens in plant material and seeds for routine diagnosis. The detection limits for both pathogens were 10 CFU/ml for cell suspensions and 1 fg of DNA, whereas in conventional PCR, the primers detected up to 105 CFU/ml and 1 ng of DNA. Specificity was confirmed by testing DNA from bean leaves, other Xanthomonas species, common fungal and bacterial bean pathogens, and bacteria from the leaf microbiota. The method was tested with bean leaves inoculated with Xpp, and the pathogen could be detected from 4 h up to 15 days postinoculation, even before disease symptoms were visible. When the method was applied to bacterium detection (Xpp or Xcf) in seed lots from infected plants, the bacterium detection rate was 100% (24 of 24). The pathogens were detected in seeds incubated for just 1 h in saline solution (0.85%), reducing the time needed for bacterium detection. The LAMP assay could be useful as a tool in bean bacterial blight management. Rapid and sensitive detection of bacteria in bean seed lots would reduce the risks of planting highly contaminated seeds in environments favorable to blight multiplication. |
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Thesagro: |
Bactéria; Doença de Planta; Feijão; Phaseolus Vulgaris; Xanthomonas Phaseoli. |
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Thesaurus Nal: |
Bacterial diseases of plants; Beans; Fields; Loop-mediated isothermal amplification; Oilseeds; Pathogen identification; Plant diseases and disorders. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02520naa a2200325 a 4500
001 2114711
005 2020-04-24
008 2020 bl uuuu u00u1 u #d
022 $a0191-2917
024 7 $a10.1094/PDIS-02-19-0325-RE$2DOI
100 1 $aPAIVA, B. A. R. de
245 $aRapid detection of Xanthomonas citri pv. fuscans and Xanthomonas phaseoli pv. phaseoli in common bean by loop-mediated isothermal amplification.$h[electronic resource]
260 $c2020
520 $aA single loop-mediated isothermal amplification (LAMP) assay was developed for specific detection of both pathogens that cause bacterial blight in common bean, Xanthomonas phaseoli pv. phaseoli (Xpp) and Xanthomonas citri pv. fuscans (Xcf). The objective was to provide a simple, easy-to-use, specific, and sensitive method to investigate the presence of one or both pathogens in plant material and seeds for routine diagnosis. The detection limits for both pathogens were 10 CFU/ml for cell suspensions and 1 fg of DNA, whereas in conventional PCR, the primers detected up to 105 CFU/ml and 1 ng of DNA. Specificity was confirmed by testing DNA from bean leaves, other Xanthomonas species, common fungal and bacterial bean pathogens, and bacteria from the leaf microbiota. The method was tested with bean leaves inoculated with Xpp, and the pathogen could be detected from 4 h up to 15 days postinoculation, even before disease symptoms were visible. When the method was applied to bacterium detection (Xpp or Xcf) in seed lots from infected plants, the bacterium detection rate was 100% (24 of 24). The pathogens were detected in seeds incubated for just 1 h in saline solution (0.85%), reducing the time needed for bacterium detection. The LAMP assay could be useful as a tool in bean bacterial blight management. Rapid and sensitive detection of bacteria in bean seed lots would reduce the risks of planting highly contaminated seeds in environments favorable to blight multiplication.
650 $aBacterial diseases of plants
650 $aBeans
650 $aFields
650 $aLoop-mediated isothermal amplification
650 $aOilseeds
650 $aPathogen identification
650 $aPlant diseases and disorders
650 $aBactéria
650 $aDoença de Planta
650 $aFeijão
650 $aPhaseolus Vulgaris
650 $aXanthomonas Phaseoli
700 1 $aWENDLAND, A.
700 1 $aTEIXEIRA, N. C.
700 1 $aFERREIRA, M. A. S. V.
773 $tPlant Disease$gv. 104, n. 1, p. 198-203, Jan. 2020.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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Biblioteca |
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Origem |
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Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
22/01/2015 |
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Data da última atualização: |
04/03/2015 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
PARRON, L. M.; FAVARETTO, N.; PORFIRIO-DA-SILVA, V.; RODRIGUES, L. N.; FRITZSONS, E.; GOMES, J. B. V. |
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Afiliação: |
LUCILIA MARIA PARRON VARGAS, CNPF; NERILDE FAVARETTO; VANDERLEY PORFIRIO DA SILVA, CNPF; LINEU NEIVA RODRIGUES, CPAC; ELENICE FRITZSONS, CNPF; JOAO BOSCO VASCONCELLOS GOMES, CNPF. |
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Título: |
Avaliação de indicadores associados à conservação dos recursos hídricos em distintos sistemas de uso da terra para valoração de serviços ambientais. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
In: SEMINÁRIO DA REDE AGROHIDRO, 1., 2012, Rio de Janeiro. Água: desafios para a sustentabilidade da agricultura: anais. Rio de Janeiro: Embrapa Solos, 2014. 83 p. (Embrapa Solos. Documentos, 167). |
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Páginas: |
p. 22-24. |
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Idioma: |
Português |
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Conteúdo: |
O objetivo do trabalho é avaliar e comparar indicadores ambientais associados aos recursos hídricos entre distintos sistemas de uso da terra, desde campo e oresta natural passando por sistemas agrosilvopastoril (uma modalidade de sistema agro orestal) e silvicultural até o outro extremo, em monocultura de plantas anuais. A quanti cação e a modelagem desta relação subsidiarão os estudos de valoração dos serviços ambientais prestados nos diferentes usos da terra. ABSTRACT - There is an association between the type and intensity of land use and the quantity and quality of environmental services generated but there are knowledge gaps of the quanti cation of environmental indicators. The objective is to evaluate and compare environmental indicators associated to water resources between different land uses systems from natural eld and forest, silvopastoral and silviculture system to annual crops. The study plots are located in Ponta Grossa, Tibagi River basin, Atlantic Forest biome, Brazil. The quanti cation and modeling these studies will support the valuation of environmental services and hydrological processes in different land uses. |
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Palavras-Chave: |
Bacia do Rio Tibagi; Mata Atlântica; Sistemas agroflorestais. |
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Thesagro: |
Escoamento; Qualidade da água. |
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Thesaurus NAL: |
Agroforestry; Runoff; Water quality. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/119257/1/2014-AAC-Lucilia-AvaliacaoIndicadores.pdf
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Marc: |
LEADER 02188nam a2200277 a 4500
001 2006438
005 2015-03-04
008 2014 bl uuuu u00u1 u #d
100 1 $aPARRON, L. M.
245 $aAvaliação de indicadores associados à conservação dos recursos hídricos em distintos sistemas de uso da terra para valoração de serviços ambientais.$h[electronic resource]
260 $aIn: SEMINÁRIO DA REDE AGROHIDRO, 1., 2012, Rio de Janeiro. Água: desafios para a sustentabilidade da agricultura: anais. Rio de Janeiro: Embrapa Solos, 2014. 83 p. (Embrapa Solos. Documentos, 167).$c2014
300 $ap. 22-24.
520 $aO objetivo do trabalho é avaliar e comparar indicadores ambientais associados aos recursos hídricos entre distintos sistemas de uso da terra, desde campo e oresta natural passando por sistemas agrosilvopastoril (uma modalidade de sistema agro orestal) e silvicultural até o outro extremo, em monocultura de plantas anuais. A quanti cação e a modelagem desta relação subsidiarão os estudos de valoração dos serviços ambientais prestados nos diferentes usos da terra. ABSTRACT - There is an association between the type and intensity of land use and the quantity and quality of environmental services generated but there are knowledge gaps of the quanti cation of environmental indicators. The objective is to evaluate and compare environmental indicators associated to water resources between different land uses systems from natural eld and forest, silvopastoral and silviculture system to annual crops. The study plots are located in Ponta Grossa, Tibagi River basin, Atlantic Forest biome, Brazil. The quanti cation and modeling these studies will support the valuation of environmental services and hydrological processes in different land uses.
650 $aAgroforestry
650 $aRunoff
650 $aWater quality
650 $aEscoamento
650 $aQualidade da água
653 $aBacia do Rio Tibagi
653 $aMata Atlântica
653 $aSistemas agroflorestais
700 1 $aFAVARETTO, N.
700 1 $aPORFIRIO-DA-SILVA, V.
700 1 $aRODRIGUES, L. N.
700 1 $aFRITZSONS, E.
700 1 $aGOMES, J. B. V.
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Embrapa Florestas (CNPF) |
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