02520naa a2200325 a 450000100080000000500110000800800410001902200140006002400360007410000230011024501720013326000090030552014940031465000330180865000100184165000110185165000430186265000130190565000280191865000330194665000140197965000220199365000120201565000230202765000250205070000170207570000200209270000260211277300560213821147112020-04-24       2020    bl uuuu     u00u1 u    #d  a0191-29177 a10.1094/PDIS-02-19-0325-RE2DOI1 aPAIVA, B. A. R. de  aRapid detection of Xanthomonas citri pv. fuscans and Xanthomonas phaseoli pv. phaseoli in common bean by loop-mediated isothermal amplification.h[electronic resource]  c2020  aA single loop-mediated isothermal amplification (LAMP) assay was developed for specific detection of both pathogens that cause bacterial blight in common bean, Xanthomonas phaseoli pv. phaseoli (Xpp) and Xanthomonas citri pv. fuscans (Xcf). The objective was to provide a simple, easy-to-use, specific, and sensitive method to investigate the presence of one or both pathogens in plant material and seeds for routine diagnosis. The detection limits for both pathogens were 10 CFU/ml for cell suspensions and 1 fg of DNA, whereas in conventional PCR, the primers detected up to 105 CFU/ml and 1 ng of DNA. Specificity was confirmed by testing DNA from bean leaves, other Xanthomonas species, common fungal and bacterial bean pathogens, and bacteria from the leaf microbiota. The method was tested with bean leaves inoculated with Xpp, and the pathogen could be detected from 4 h up to 15 days postinoculation, even before disease symptoms were visible. When the method was applied to bacterium detection (Xpp or Xcf) in seed lots from infected plants, the bacterium detection rate was 100% (24 of 24). The pathogens were detected in seeds incubated for just 1 h in saline solution (0.85%), reducing the time needed for bacterium detection. The LAMP assay could be useful as a tool in bean bacterial blight management. Rapid and sensitive detection of bacteria in bean seed lots would reduce the risks of planting highly contaminated seeds in environments favorable to blight multiplication.  aBacterial diseases of plants  aBeans  aFields  aLoop-mediated isothermal amplification  aOilseeds  aPathogen identification  aPlant diseases and disorders  aBactéria  aDoença de Planta  aFeijão  aPhaseolus Vulgaris  aXanthomonas Phaseoli1 aWENDLAND, A.1 aTEIXEIRA, N. C.1 aFERREIRA, M. A. S. V.  tPlant Diseasegv. 104, n. 1, p. 198-203, Jan. 2020.