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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
30/08/2018 |
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Data da última atualização: |
30/08/2018 |
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Autoria: |
BOTELHO, A. F. M.; OLIVEIRA, F. A. S.; FIÚZA, A. T. L.; PEDROSA, H. P.; BRANCO, S. E. M. T.; PIEREZAN, F.; MELO, M. M.; SOTO-BLANCO, B. |
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Afiliação: |
Ana F. M. Botelho, Departamento de Clínica e Cirurgia Veterinárias/Escola de Veterinária/Universidade Federal de Minas Gerais - UFMG; Fabiano A. S. Oliveira, Laboratório Nacional Agropecuário - Lanagro/MG/Ministério da Agricultura, Pecuária e Abastecimento; Aparecida T. L. Fiúza, Departamento de Clínica e Cirurgia Veterinárias/Escola de Veterinária/Universidade Federal de Minas Gerais - UFMG; Heloísa P. Pedroza, Departamento de Clínica e Cirurgia Veterinárias/Escola de Veterinária/Universidade Federal de Minas Gerais - UFMG; Stephanie E. M. T. Branco, Departamento de Clínica e Cirurgia Veterinárias/Escola de Veterinária/Universidade Federal de Minas Gerais - UFMG; Felipe Pierezan, Departamento de Clínica e Cirurgia Veterinárias/Escola de Veterinária/Universidade Federal de Minas Gerais - UFMG; Marília M. Melo, Departamento de Clínica e Cirurgia Veterinárias/Escola de Veterinária/Universidade Federal de Minas Gerais - UFMG; Benito Soto-Blanco, Departamento de Clínica e Cirurgia Veterinárias/Escola de Veterinária/Universidade Federal de Minas Gerais - UFMG. |
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Título: |
Improved method for diagnosis of Nerium oleander poisoning in necropsy tissues. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 5, p. 967-972, maio 2018 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Método aprimorado para diagnóstico da intoxicação por Nerium oleander em tecidos de necropsia. |
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Conteúdo: |
Nerium oleander is an ornamental cardiotoxic plant found in tropical and subtropical areas of the World. Its toxicity is related to the content of cardioactive glycosides, mainly oleandrin, found throughout the plant. The present study aimed to describe a new and improved method for oleandrin detection in tissue samples. The determination of oleandrin was made after extraction with a modified QuEChERS technique and measurement by UFLC-MS/MS. A total of 36 guinea pigs (Cavia porcellus) were distributed into 3 groups (n=12): control group that received only water orally (CON), and two treated groups that received hydroalcoholic oleander extract at doses of 150mg.kg-1 (OLE 150) and 300mg.kg-1 (OLE 300) in single oral dose. After three hours, fragments of heart, kidneys, liver and brain were collected for determination of oleandrin levels. The extraction and chromatographic procedures were effective for oleandrin detection and quantification in tissues, with retention time of 1.2 min and detection limit of 0.001μg g-1. The chromatographic analysis of treated guinea pigs indicated that oleandrin is distributed equally among the analyzed tissues. The developed methodology is a reliable, effective and rapid form of diagnosis of N. oleander poisoning based on necropsy tissue samples. |
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Palavras-Chave: |
Cromatografia líquida; Espectrometria de massa; Teste diagnóstico; Toxicose. |
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Thesagro: |
Cobaia; Oleander. |
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Thesaurus Nal: |
Cardenolides; Guinea pigs; Liquid chromatography; Mass spectrometry; Nerium oleander. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/182115/1/Improved-method-for-diagnosis-of-nerium-oleander.pdf
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Marc: |
LEADER 02425naa a2200349 a 4500
001 2094762
005 2018-08-30
008 2018 bl uuuu u00u1 u #d
100 1 $aBOTELHO, A. F. M.
245 $aImproved method for diagnosis of Nerium oleander poisoning in necropsy tissues.$h[electronic resource]
260 $c2018
500 $aTítulo em português: Método aprimorado para diagnóstico da intoxicação por Nerium oleander em tecidos de necropsia.
520 $aNerium oleander is an ornamental cardiotoxic plant found in tropical and subtropical areas of the World. Its toxicity is related to the content of cardioactive glycosides, mainly oleandrin, found throughout the plant. The present study aimed to describe a new and improved method for oleandrin detection in tissue samples. The determination of oleandrin was made after extraction with a modified QuEChERS technique and measurement by UFLC-MS/MS. A total of 36 guinea pigs (Cavia porcellus) were distributed into 3 groups (n=12): control group that received only water orally (CON), and two treated groups that received hydroalcoholic oleander extract at doses of 150mg.kg-1 (OLE 150) and 300mg.kg-1 (OLE 300) in single oral dose. After three hours, fragments of heart, kidneys, liver and brain were collected for determination of oleandrin levels. The extraction and chromatographic procedures were effective for oleandrin detection and quantification in tissues, with retention time of 1.2 min and detection limit of 0.001μg g-1. The chromatographic analysis of treated guinea pigs indicated that oleandrin is distributed equally among the analyzed tissues. The developed methodology is a reliable, effective and rapid form of diagnosis of N. oleander poisoning based on necropsy tissue samples.
650 $aCardenolides
650 $aGuinea pigs
650 $aLiquid chromatography
650 $aMass spectrometry
650 $aNerium oleander
650 $aCobaia
650 $aOleander
653 $aCromatografia líquida
653 $aEspectrometria de massa
653 $aTeste diagnóstico
653 $aToxicose
700 1 $aOLIVEIRA, F. A. S.
700 1 $aFIÚZA, A. T. L.
700 1 $aPEDROSA, H. P.
700 1 $aBRANCO, S. E. M. T.
700 1 $aPIEREZAN, F.
700 1 $aMELO, M. M.
700 1 $aSOTO-BLANCO, B.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 38, n. 5, p. 967-972, maio 2018
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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