02425naa a2200349 a 450000100080000000500110000800800410001910000220006024501070008226000090018950001260019852013060032465000170163065000160164765000260166365000220168965000200171165000110173165000130174265300270175565300280178265300230181065300130183370000230184670000210186970000190189070000240190970000170193370000160195070000200196677300890198620947622018-08-30       2018    bl uuuu     u00u1 u    #d1 aBOTELHO, A. F. M.  aImproved method for diagnosis of Nerium oleander poisoning in necropsy tissues.h[electronic resource]  c2018  aTítulo em português: Método aprimorado para diagnóstico da intoxicação por Nerium oleander em tecidos de necropsia.  aNerium oleander is an ornamental cardiotoxic plant found in tropical and subtropical areas of the World. Its toxicity is related to the content of cardioactive glycosides, mainly oleandrin, found throughout the plant. The present study aimed to describe a new and improved method for oleandrin detection in tissue samples. The determination of oleandrin was made after extraction with a modified QuEChERS technique and measurement by UFLC-MS/MS. A total of 36 guinea pigs (Cavia porcellus) were distributed into 3 groups (n=12): control group that received only water orally (CON), and two treated groups that received hydroalcoholic oleander extract at doses of 150mg.kg-1 (OLE 150) and 300mg.kg-1 (OLE 300) in single oral dose. After three hours, fragments of heart, kidneys, liver and brain were collected for determination of oleandrin levels. The extraction and chromatographic procedures were effective for oleandrin detection and quantification in tissues, with retention time of 1.2 min and detection limit of 0.001μg g-1. The chromatographic analysis of treated guinea pigs indicated that oleandrin is distributed equally among the analyzed tissues. The developed methodology is a reliable, effective and rapid form of diagnosis of N. oleander poisoning based on necropsy tissue samples.  aCardenolides  aGuinea pigs  aLiquid chromatography  aMass spectrometry  aNerium oleander  aCobaia  aOleander  aCromatografia líquida  aEspectrometria de massa  aTeste diagnóstico  aToxicose1 aOLIVEIRA, F. A. S.1 aFIÚZA, A. T. L.1 aPEDROSA, H. P.1 aBRANCO, S. E. M. T.1 aPIEREZAN, F.1 aMELO, M. M.1 aSOTO-BLANCO, B.  tPesquisa Veterinária Brasileira, Rio de Janeirogv. 38, n. 5, p. 967-972, maio 2018