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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia; Embrapa Agroindústria de Alimentos. |
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Data corrente: |
19/09/2017 |
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Data da última atualização: |
08/12/2017 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
TAMIETTI, M. S.; MIDORIKAWA, G. E. O.; MENDES, T. D.; DAMASO, M. C. T.; BOM, E. P. S.; SILVA, A. S.; GOTTSCHALK, L. M. F.; NORONHA, E. F.; FAVARO, L. C. de L. |
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Afiliação: |
MARIANA S. TAMIETTI, UNB; GLÁUCIA E. O. MIDORIKAWA; THAIS DEMARCHI MENDES, CNPAE; MONICA CARAMEZ TRICHES DAMASO, CNPAE; ELBA P. S. BOM, UFRJ; AYLA S. SILVA, INT; LEDA MARIA FORTES GOTTSCHALK, CTAA; ELIANE F. NORONHA, UNB; LEIA CECILIA DE LIMA FAVARO, CNPAE. |
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Título: |
Enhancement of Trichoderma harzianum CFAM-422 for cellulase and hemicellulase production by deletion of the carbon catabolite repressor gene cre1. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
In: SIMPÓSIO NACIONAL DE BIOPROCESSOS, 21.; SIMPÓSIO DE HIDRÓLISE ENZIMÁTICA DE BIOMASSA, 12., 2017, Aracaju, SE. [Proceedings ...]. São Paulo: Associação Brasileira de Engenharia Química, 2017. |
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Páginas: |
Não paginado. |
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ISSN: |
2447-2816 |
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Idioma: |
Inglês |
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Notas: |
SINAFERM; SHEB. 3 a 6 de setembro. Seção Trabalhos. Ref. 59019. |
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Conteúdo: |
Carbon catabolite repression (CCR) is a mechanism by which microorganisms can utilize preferably highly energetic compounds over those of difficult degradation. For Trichoderma reesei, the protein that acts as repressor in the presence of glucose is CRE1. In this project, we aim to delete cre1 gene in Trichoderma harzianum CFAM-422 and obtain mutants with enhanced production of biomass degrading enzymes. Disruption of cre1 in T. harzianum CFAM-422 was performed by gene replacement of cre1 for hph (hygromycin B phosphotransferase) via homologous recombination. Hygromycin resistant mutants and parental strains enzyme production was evaluated in both inductive and repressive conditions in four different carbon sources. Enzymatic indexes (EI) were determined and compared. All genetically stable transformants showed increased enzymatic index under inductive conditions and modest inhibition under repressive conditions for most carbon sources, indicating that the deletion of cre1 in T. harzianum can be beneficial to cellulase and hemicellulase production with reduced product inhibition. |
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Palavras-Chave: |
Carbon Catabolite Repression; Cellulolytic and hemicellulolytic enzymes; Processos de hidrolise enzimática de biomassa. |
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Thesagro: |
Celulase; Trichoderma Harzianum. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/164843/1/galoa-proceedings-sinaferm-2017-59019-enhancement-of-t.pdf
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Marc: |
LEADER 02302nam a2200301 a 4500
001 2076994
005 2017-12-08
008 2017 bl uuuu u00u1 u #d
022 $a2447-2816
100 1 $aTAMIETTI, M. S.
245 $aEnhancement of Trichoderma harzianum CFAM-422 for cellulase and hemicellulase production by deletion of the carbon catabolite repressor gene cre1.$h[electronic resource]
260 $aIn: SIMPÓSIO NACIONAL DE BIOPROCESSOS, 21.; SIMPÓSIO DE HIDRÓLISE ENZIMÁTICA DE BIOMASSA, 12., 2017, Aracaju, SE. [Proceedings ...]. São Paulo: Associação Brasileira de Engenharia Química$c2017
300 $aNão paginado.
500 $aSINAFERM; SHEB. 3 a 6 de setembro. Seção Trabalhos. Ref. 59019.
520 $aCarbon catabolite repression (CCR) is a mechanism by which microorganisms can utilize preferably highly energetic compounds over those of difficult degradation. For Trichoderma reesei, the protein that acts as repressor in the presence of glucose is CRE1. In this project, we aim to delete cre1 gene in Trichoderma harzianum CFAM-422 and obtain mutants with enhanced production of biomass degrading enzymes. Disruption of cre1 in T. harzianum CFAM-422 was performed by gene replacement of cre1 for hph (hygromycin B phosphotransferase) via homologous recombination. Hygromycin resistant mutants and parental strains enzyme production was evaluated in both inductive and repressive conditions in four different carbon sources. Enzymatic indexes (EI) were determined and compared. All genetically stable transformants showed increased enzymatic index under inductive conditions and modest inhibition under repressive conditions for most carbon sources, indicating that the deletion of cre1 in T. harzianum can be beneficial to cellulase and hemicellulase production with reduced product inhibition.
650 $aCelulase
650 $aTrichoderma Harzianum
653 $aCarbon Catabolite Repression
653 $aCellulolytic and hemicellulolytic enzymes
653 $aProcessos de hidrolise enzimática de biomassa
700 1 $aMIDORIKAWA, G. E. O.
700 1 $aMENDES, T. D.
700 1 $aDAMASO, M. C. T.
700 1 $aBOM, E. P. S.
700 1 $aSILVA, A. S.
700 1 $aGOTTSCHALK, L. M. F.
700 1 $aNORONHA, E. F.
700 1 $aFAVARO, L. C. de L.
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Registro original: |
Embrapa Agroindústria de Alimentos (CTAA) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
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URL |
Voltar
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com cpac.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
17/05/2021 |
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Data da última atualização: |
17/05/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 3 |
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Autoria: |
CARVALHO, A. M. de; OLIVEIRA, A. D. de; COSER, T. R.; SOUSA, T. R. de; LIMA, C. A. de; RAMOS, M. L. G.; MALAQUIAS, J. V.; GONÇALVES, A. D. M. de A.; RIBEIRO JUNIOR, W. Q. |
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Afiliação: |
ARMINDA MOREIRA DE CARVALHO, CPAC; ALEXSANDRA DUARTE DE OLIVEIRA, CPAC; THAIS RODRIGUES COSER; THAIS RODRIGUES DE SOUSA; CRISTIANE ANDREA DE LIMA; MARIA LUCRÉCIA GEROSA RAMOS; JUACI VITORIA MALAQUIAS, CPAC; ADRIANO DICESAR MARTINS DE ARAUJO GONÇALVES; WALTER QUADROS RIBEIRO JUNIOR, CPAC. |
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Título: |
N2O emissions from sugarcane fields under contrasting watering regimes in the Brazilian savannah. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Environmental Technology & Innovation, v. 22, n. 101470, 2021. |
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Páginas: |
11 p. |
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DOI: |
https://doi.org/10.1016/j.eti.2021.101470 2352-1864/ |
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Idioma: |
Inglês |
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Conteúdo: |
Bioethanol and other sugarcane by-products, such as the bagasse, are responsible for approximately 12% of Brazil?s total energy generation. This crop is expanding in the Central-Western Region of Brazil, within the Brazilian savannah biome (Cerrado). It is strongly associated with the use of mineral and/or organic fertilizers and irrigation practices due to drought period during winter. However, higher soil moisture content (due to rain or irrigation) and nitrogen (N) application increase nitrous oxide (N2O) emission, which is one of the main greenhouse gases from the agricultural sector. This study aimed to evaluate soil N2O fluxes in sugarcane under field conditions with contrasting water regimes in the Cerrado region of Brazil. Due to watering regimes, based on different crop evapotranspiration demand, it was possible to establish adequate water-use efficiency technologies. Increases in watering regimes did not increase N2O emissions compared to ??rescue irrigation??, commonly used by farmers in the region. However, higher yields were obtained from the higher watering regime (T75%) compared to the farmers? common practice. |
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Thesagro: |
Efeito Estufa; Fertilizante Nitrogenado; Irrigação; Latossolo. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02048naa a2200289 a 4500
001 2131838
005 2021-05-17
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.eti.2021.101470 2352-1864/$2DOI
100 1 $aCARVALHO, A. M. de
245 $aN2O emissions from sugarcane fields under contrasting watering regimes in the Brazilian savannah.$h[electronic resource]
260 $c2021
300 $a11 p.
520 $aBioethanol and other sugarcane by-products, such as the bagasse, are responsible for approximately 12% of Brazil?s total energy generation. This crop is expanding in the Central-Western Region of Brazil, within the Brazilian savannah biome (Cerrado). It is strongly associated with the use of mineral and/or organic fertilizers and irrigation practices due to drought period during winter. However, higher soil moisture content (due to rain or irrigation) and nitrogen (N) application increase nitrous oxide (N2O) emission, which is one of the main greenhouse gases from the agricultural sector. This study aimed to evaluate soil N2O fluxes in sugarcane under field conditions with contrasting water regimes in the Cerrado region of Brazil. Due to watering regimes, based on different crop evapotranspiration demand, it was possible to establish adequate water-use efficiency technologies. Increases in watering regimes did not increase N2O emissions compared to ??rescue irrigation??, commonly used by farmers in the region. However, higher yields were obtained from the higher watering regime (T75%) compared to the farmers? common practice.
650 $aEfeito Estufa
650 $aFertilizante Nitrogenado
650 $aIrrigação
650 $aLatossolo
700 1 $aOLIVEIRA, A. D. de
700 1 $aCOSER, T. R.
700 1 $aSOUSA, T. R. de
700 1 $aLIMA, C. A. de
700 1 $aRAMOS, M. L. G.
700 1 $aMALAQUIAS, J. V.
700 1 $aGONÇALVES, A. D. M. de A.
700 1 $aRIBEIRO JUNIOR, W. Q.
773 $tEnvironmental Technology & Innovation$gv. 22, n. 101470, 2021.
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Registro original: |
Embrapa Cerrados (CPAC) |
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