| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Meio Norte / UEP-Parnaíba. |
|
Data corrente: |
16/07/1993 |
|
Data da última atualização: |
26/08/2009 |
|
Autoria: |
IWATSUBO, T.; NAKAGAWA, H.; OGURA, N.; HIRABAYASHI, T.; SATO, T. |
|
Título: |
Acid invertase of melon fruits: immunochemical detection of acid invertases. |
|
Ano de publicação: |
1992 |
|
Fonte/Imprenta: |
Plant Cell Physiology, v. 33, n. 8, p. 1127-1133, 1992. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Soluble (S) and cell wall-bound (CW) forms of acid invertases (AIs) were partially purified from mesocarp tissue of melon (Cucumis melo L. cv. AMS) fruits and some properties of the enzymes were examined. The optimum pH values for activity were 5.5 and 4.5 for S- and CW-AI, respectively. Km values of S- and CW-AIs for sucrose were 4.2 and 1.7 mM, and those for raffinose were 20.0 and 10.5 mM, respectively. The band of a polypeptide od about 70 kDa was detected with antibodies against AI of tomato fruits (CW form) and of mung bean seedlings (S form) by immunoblot analysis. The 70-kDa polypeptide was isolated from each fraction and further analyzed by limited proteolytic digestion with Staphylococcus aureus V8 protease. The digested polypeptides generated almost identical profiles. The localization of CW-AI was also studied and it was found to be an extracellular enzyme. The activities of S- and CW-AIs per gram fresh weight increased and reached a maximum on day after anthesis, and then they decreased rapidly during maturation of fruits. The relative levels of S- and CW-AI polypeptides were correlated with the levels of activities of S-and CW-AIs. These results show that the decrease in acitvity of AI during maturation of melon fruits is due to a decline in levels of AI polypeptides in the fruits. |
|
Palavras-Chave: |
Defecção; Defection. |
|
Thesagro: |
Fisiologia Vegetal; Melão. |
|
Thesaurus Nal: |
plant physiology. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 01927naa a2200229 a 4500
001 1073447
005 2009-08-26
008 1992 bl --- 0-- u #d
100 1 $aIWATSUBO, T.
245 $aAcid invertase of melon fruits$bimmunochemical detection of acid invertases.
260 $c1992
520 $aSoluble (S) and cell wall-bound (CW) forms of acid invertases (AIs) were partially purified from mesocarp tissue of melon (Cucumis melo L. cv. AMS) fruits and some properties of the enzymes were examined. The optimum pH values for activity were 5.5 and 4.5 for S- and CW-AI, respectively. Km values of S- and CW-AIs for sucrose were 4.2 and 1.7 mM, and those for raffinose were 20.0 and 10.5 mM, respectively. The band of a polypeptide od about 70 kDa was detected with antibodies against AI of tomato fruits (CW form) and of mung bean seedlings (S form) by immunoblot analysis. The 70-kDa polypeptide was isolated from each fraction and further analyzed by limited proteolytic digestion with Staphylococcus aureus V8 protease. The digested polypeptides generated almost identical profiles. The localization of CW-AI was also studied and it was found to be an extracellular enzyme. The activities of S- and CW-AIs per gram fresh weight increased and reached a maximum on day after anthesis, and then they decreased rapidly during maturation of fruits. The relative levels of S- and CW-AI polypeptides were correlated with the levels of activities of S-and CW-AIs. These results show that the decrease in acitvity of AI during maturation of melon fruits is due to a decline in levels of AI polypeptides in the fruits.
650 $aplant physiology
650 $aFisiologia Vegetal
650 $aMelão
653 $aDefecção
653 $aDefection
700 1 $aNAKAGAWA, H.
700 1 $aOGURA, N.
700 1 $aHIRABAYASHI, T.
700 1 $aSATO, T.
773 $tPlant Cell Physiology$gv. 33, n. 8, p. 1127-1133, 1992.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Meio Norte / UEP-Parnaíba (CPAMN-UEPP) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Café. |
|
Data corrente: |
09/01/2018 |
|
Data da última atualização: |
10/01/2018 |
|
Tipo da produção científica: |
Resumo em Anais de Congresso |
|
Autoria: |
MATTOS, N. G.; ANDRADE, A. C. |
|
Afiliação: |
Nathália Gomes Mattos, UFV; ALAN CARVALHO ANDRADE, SAPC. |
|
Título: |
Development of a molecular method for the identification of clone varieties of Coffea canephora via PCR. |
|
Ano de publicação: |
2017 |
|
Fonte/Imprenta: |
In: SIMPÓSIO INTERNACIONAL DE GENÉTICA E MELHORAMENTO, 8., 2017, Viçosa, MG. Ômicas: do gene ao fenótipo. [Proceedings...] Viçosa, MG: UFV, 2017. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Conilon coffee belongs to the Coffea canephora species, which is a diploid (2n = 2 × = 22), perennial, allogamous plant with a high genetic variability. Consequently, a plantation established from seeds will likely have plants with highly diversified characteristics. Accordingly, the production of cuttings is of great value for the success of crop implementation. Using clones produced from superior genotypes instead of seeds has some advantages such as lower implementation costs, higher productivity, higher uniformity of maturation, better grain quality, differentiated cycle of maturation and staggered harvest scheduling. Brazil is an important coffee producer, and the development of new varieties is important to maintain or even increase its production. |
|
Palavras-Chave: |
Embrapa; Genetic variability; New cultivar. |
|
Thesagro: |
Coffea Canephora. |
|
Thesaurus NAL: |
Brazil; Clones; Intellectual property rights. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/170612/1/Development-of-a-molecular-method-for-the-identification-of-clone.pdf
|
|
Marc: |
LEADER 01489nam a2200205 a 4500
001 2084631
005 2018-01-10
008 2017 bl uuuu u00u1 u #d
100 1 $aMATTOS, N. G.
245 $aDevelopment of a molecular method for the identification of clone varieties of Coffea canephora via PCR.$h[electronic resource]
260 $aIn: SIMPÓSIO INTERNACIONAL DE GENÉTICA E MELHORAMENTO, 8., 2017, Viçosa, MG. Ômicas: do gene ao fenótipo. [Proceedings...] Viçosa, MG: UFV$c2017
520 $aConilon coffee belongs to the Coffea canephora species, which is a diploid (2n = 2 × = 22), perennial, allogamous plant with a high genetic variability. Consequently, a plantation established from seeds will likely have plants with highly diversified characteristics. Accordingly, the production of cuttings is of great value for the success of crop implementation. Using clones produced from superior genotypes instead of seeds has some advantages such as lower implementation costs, higher productivity, higher uniformity of maturation, better grain quality, differentiated cycle of maturation and staggered harvest scheduling. Brazil is an important coffee producer, and the development of new varieties is important to maintain or even increase its production.
650 $aBrazil
650 $aClones
650 $aIntellectual property rights
650 $aCoffea Canephora
653 $aEmbrapa
653 $aGenetic variability
653 $aNew cultivar
700 1 $aANDRADE, A. C.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Café (CNPCa) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
