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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
28/09/2016 |
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Data da última atualização: |
24/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, C. R. C.; MONNERAT, R.; LIMA, L. M. de; MARTINS, E. S.; MELO FILHO, P. A.; PINHEIRO, M. P. N.; SANTOS, R. C. |
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Afiliação: |
CARLIANE R. C. SILVA, UFRPE; ROSE GOMES MONNERAT SOLON DE PONTES, CENARGEN; LIZIANE MARIA DE LIMA, CNPA; ÉRICA S. MARTINS, CENARGEN; PÉRICLES A. MELO FIILHO, UFRPE; MORGANNA P. N. PINHEIRO, UFRPE; ROSEANE CAVALCANTI DOS SANTOS, CNPA. |
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Título: |
Stable integration and expression of a cry1la gene conferring resistance to fall armyworm and boll weevil in cotton plants. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Pest Management Science, v. 72, p. 1549-1557, 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
BACKGROUND: Boll weevil is a serious pest of cotton crop. Effective control involves applications of chemical insecticides, increasing the cost of production and environmental pollution. The current genetically modified Bt crops have allowed great benefits to farmers but show activity limited to lepidopteran pests. This work reports on procedures adopted for integration and expression of a cry transgene conferring resistance to boll weevil and fall armyworm by usingmolecular tools. RESULTS: Four Brazilian cotton cultivars were microinjected with a minimal linear cassette generating 1248 putative lines. Complete gene integration was found in only one line (T0-34) containing one copy of cry1Ia detected by Southern blot. Protein was expressed in high concentration at 45 days after emergence (dae), decreasing by approximately 50% at 90 dae. Toxicity of the cry protein was demonstrated in feeding bioassays revealing 56.7% mortality to boll weevil fed buds and 88.1% mortality to fall armyworm fed leaves. A binding of cry1Ia antibody was found in the midgut of boll weevils fed on T0-34 buds in an immunodetection assay. CONCLUSION: The gene introduced into plants confers resistance to boll weevil and fall armyworm. Transmission of the transgene occurred normally to T1 progeny.All plants showed phenotypically normal growth,with fertile flowers and abundant seeds. |
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Palavras-Chave: |
Chemical inseticides. |
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Thesagro: |
Anthonomus grandis; Gossypium hirsutum; Spodoptera frugiperda. |
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Thesaurus Nal: |
cotton. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/183293/1/Silva-et-al-2016-Pest-Management-Science.pdf
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Marc: |
LEADER 02157naa a2200253 a 4500
001 2053604
005 2023-03-24
008 2016 bl uuuu u00u1 u #d
100 1 $aSILVA, C. R. C.
245 $aStable integration and expression of a cry1la gene conferring resistance to fall armyworm and boll weevil in cotton plants.$h[electronic resource]
260 $c2016
520 $aBACKGROUND: Boll weevil is a serious pest of cotton crop. Effective control involves applications of chemical insecticides, increasing the cost of production and environmental pollution. The current genetically modified Bt crops have allowed great benefits to farmers but show activity limited to lepidopteran pests. This work reports on procedures adopted for integration and expression of a cry transgene conferring resistance to boll weevil and fall armyworm by usingmolecular tools. RESULTS: Four Brazilian cotton cultivars were microinjected with a minimal linear cassette generating 1248 putative lines. Complete gene integration was found in only one line (T0-34) containing one copy of cry1Ia detected by Southern blot. Protein was expressed in high concentration at 45 days after emergence (dae), decreasing by approximately 50% at 90 dae. Toxicity of the cry protein was demonstrated in feeding bioassays revealing 56.7% mortality to boll weevil fed buds and 88.1% mortality to fall armyworm fed leaves. A binding of cry1Ia antibody was found in the midgut of boll weevils fed on T0-34 buds in an immunodetection assay. CONCLUSION: The gene introduced into plants confers resistance to boll weevil and fall armyworm. Transmission of the transgene occurred normally to T1 progeny.All plants showed phenotypically normal growth,with fertile flowers and abundant seeds.
650 $acotton
650 $aAnthonomus grandis
650 $aGossypium hirsutum
650 $aSpodoptera frugiperda
653 $aChemical inseticides
700 1 $aMONNERAT, R.
700 1 $aLIMA, L. M. de
700 1 $aMARTINS, E. S.
700 1 $aMELO FILHO, P. A.
700 1 $aPINHEIRO, M. P. N.
700 1 $aSANTOS, R. C.
773 $tPest Management Science$gv. 72, p. 1549-1557, 2016.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
12/09/2021 |
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Data da última atualização: |
07/12/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
BARROS, R. de A.; VITAL, C. E.; SILVA JÚNIOR, N. R.; VARGAS, M. A. S.; MONTEIRO, L. P.; FAUSTINO, V. A.; AUAD, A. M.; PEREIRA, J. F.; OLIVEIRA, E. E. de; RAMOS, H. J. O.; OLIVEIRA, M. G. de A. |
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Afiliação: |
RAFAEL DE A. BARROS, Universidade Federal de Viçosa; CAMILO E. VITAL, Universidade Federal de Viçosa; NEILIER R. SILVA JÚNIOR, Universidade Federal de Viçosa; MANUEL A. S. VARGAS, Universidade Federal de Viçosa; LUANA P. MONTEIRO, Universidade Federal de Viçosa; VERONICA A. FAUSTINO, Universidade Federal de Viçosa; ALEXANDER MACHADO AUAD, CNPGL; JORGE FERNANDO PEREIRA, CNPGL; EUGENIO E. DE OLIVEIRA, Universidade Federal de Viçosa; HUMBERTO J. O. RAMOS, Universidade Federal de Viçosa; MARIA GORETI DE A. OLIVEIRA, Universidade Federal de Viçosa. |
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Título: |
Differential defense responses of tropical grasses to Mahanarva spectabilis (Hemiptera: Cercopidae) infestation. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Anais da Academia Brasileira de Ciências, v. 93, n. 3, e20191456, 2021. |
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DOI: |
https://doi.org/10.1590/0001-3765202120191456 |
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Idioma: |
Inglês |
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Conteúdo: |
The spittlebugs Mahanarva spectabilis economically challenges cattle production of neotropical regions, due to its voracious feeding on tropical grasses. Here, we evaluated biochemical responses of the interaction between M. spectabilis and the widely cultivated tropical grasses Brachiaria spp. (i.e., brizantha and decumbens) and elephant grasses (cvs. Roxo de Botucatu and Pioneiro), regarding lipoxygenases, protease inhibitors, phytohormones, and proteolytic activities in the midgut of M. spectabilis. The M. spectabilis-infested grasses increased lipoxygenases activity, except for cv. Pioneiro. The levels of the phytohormones jasmonic and abscisic acids were similarly low in all genotypes and increased under herbivory. Furthermore, salicylic acid concentration was constitutively higher in Brachiaria sp., increasing only in spittlebug-infested B. decumbens. M. spectabilis infestations did not induce increases of protease inhibitors in any forage grass type. The trypsin activity remained unaltered, and the total proteolytic activity increased only in B decumbens-fed insects. Our findings revealed that most forage grasses exposed to spittlebugs activate the lipoxygenases pathway, resulting in increased abscisic and jasmonic acids. However, greater amounts of these hormones do not induce protease inhibitory activity in response to spittlebug attack. This knowledge certainly helps to guide future projects aiming at reducing the impact of spittlebugs on forage production. |
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Thesagro: |
Cigarrinha das Pastagens; Hormônio; Inseto; Intestino; Pastagem; Protease. |
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Thesaurus NAL: |
Hormones; Insects; Midgut. |
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Categoria do assunto: |
O Insetos e Entomologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/226029/1/Differential-defense.pdf
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Marc: |
LEADER 02557naa a2200361 a 4500
001 2134243
005 2021-12-07
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1590/0001-3765202120191456$2DOI
100 1 $aBARROS, R. de A.
245 $aDifferential defense responses of tropical grasses to Mahanarva spectabilis (Hemiptera$bCercopidae) infestation.$h[electronic resource]
260 $c2021
520 $aThe spittlebugs Mahanarva spectabilis economically challenges cattle production of neotropical regions, due to its voracious feeding on tropical grasses. Here, we evaluated biochemical responses of the interaction between M. spectabilis and the widely cultivated tropical grasses Brachiaria spp. (i.e., brizantha and decumbens) and elephant grasses (cvs. Roxo de Botucatu and Pioneiro), regarding lipoxygenases, protease inhibitors, phytohormones, and proteolytic activities in the midgut of M. spectabilis. The M. spectabilis-infested grasses increased lipoxygenases activity, except for cv. Pioneiro. The levels of the phytohormones jasmonic and abscisic acids were similarly low in all genotypes and increased under herbivory. Furthermore, salicylic acid concentration was constitutively higher in Brachiaria sp., increasing only in spittlebug-infested B. decumbens. M. spectabilis infestations did not induce increases of protease inhibitors in any forage grass type. The trypsin activity remained unaltered, and the total proteolytic activity increased only in B decumbens-fed insects. Our findings revealed that most forage grasses exposed to spittlebugs activate the lipoxygenases pathway, resulting in increased abscisic and jasmonic acids. However, greater amounts of these hormones do not induce protease inhibitory activity in response to spittlebug attack. This knowledge certainly helps to guide future projects aiming at reducing the impact of spittlebugs on forage production.
650 $aHormones
650 $aInsects
650 $aMidgut
650 $aCigarrinha das Pastagens
650 $aHormônio
650 $aInseto
650 $aIntestino
650 $aPastagem
650 $aProtease
700 1 $aVITAL, C. E.
700 1 $aSILVA JÚNIOR, N. R.
700 1 $aVARGAS, M. A. S.
700 1 $aMONTEIRO, L. P.
700 1 $aFAUSTINO, V. A.
700 1 $aAUAD, A. M.
700 1 $aPEREIRA, J. F.
700 1 $aOLIVEIRA, E. E. de
700 1 $aRAMOS, H. J. O.
700 1 $aOLIVEIRA, M. G. de A.
773 $tAnais da Academia Brasileira de Ciências$gv. 93, n. 3, e20191456, 2021.
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Embrapa Gado de Leite (CNPGL) |
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