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Registro Completo |
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
28/09/2016 |
Data da última atualização: |
24/03/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SILVA, C. R. C.; MONNERAT, R.; LIMA, L. M. de; MARTINS, E. S.; MELO FILHO, P. A.; PINHEIRO, M. P. N.; SANTOS, R. C. |
Afiliação: |
CARLIANE R. C. SILVA, UFRPE; ROSE GOMES MONNERAT SOLON DE PONTES, CENARGEN; LIZIANE MARIA DE LIMA, CNPA; ÉRICA S. MARTINS, CENARGEN; PÉRICLES A. MELO FIILHO, UFRPE; MORGANNA P. N. PINHEIRO, UFRPE; ROSEANE CAVALCANTI DOS SANTOS, CNPA. |
Título: |
Stable integration and expression of a cry1la gene conferring resistance to fall armyworm and boll weevil in cotton plants. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Pest Management Science, v. 72, p. 1549-1557, 2016. |
Idioma: |
Inglês |
Conteúdo: |
BACKGROUND: Boll weevil is a serious pest of cotton crop. Effective control involves applications of chemical insecticides, increasing the cost of production and environmental pollution. The current genetically modified Bt crops have allowed great benefits to farmers but show activity limited to lepidopteran pests. This work reports on procedures adopted for integration and expression of a cry transgene conferring resistance to boll weevil and fall armyworm by usingmolecular tools. RESULTS: Four Brazilian cotton cultivars were microinjected with a minimal linear cassette generating 1248 putative lines. Complete gene integration was found in only one line (T0-34) containing one copy of cry1Ia detected by Southern blot. Protein was expressed in high concentration at 45 days after emergence (dae), decreasing by approximately 50% at 90 dae. Toxicity of the cry protein was demonstrated in feeding bioassays revealing 56.7% mortality to boll weevil fed buds and 88.1% mortality to fall armyworm fed leaves. A binding of cry1Ia antibody was found in the midgut of boll weevils fed on T0-34 buds in an immunodetection assay. CONCLUSION: The gene introduced into plants confers resistance to boll weevil and fall armyworm. Transmission of the transgene occurred normally to T1 progeny.All plants showed phenotypically normal growth,with fertile flowers and abundant seeds. |
Palavras-Chave: |
Chemical inseticides. |
Thesagro: |
Anthonomus grandis; Gossypium hirsutum; Spodoptera frugiperda. |
Thesaurus Nal: |
cotton. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/183293/1/Silva-et-al-2016-Pest-Management-Science.pdf
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Marc: |
LEADER 02157naa a2200253 a 4500 001 2053604 005 2023-03-24 008 2016 bl uuuu u00u1 u #d 100 1 $aSILVA, C. R. C. 245 $aStable integration and expression of a cry1la gene conferring resistance to fall armyworm and boll weevil in cotton plants.$h[electronic resource] 260 $c2016 520 $aBACKGROUND: Boll weevil is a serious pest of cotton crop. Effective control involves applications of chemical insecticides, increasing the cost of production and environmental pollution. The current genetically modified Bt crops have allowed great benefits to farmers but show activity limited to lepidopteran pests. This work reports on procedures adopted for integration and expression of a cry transgene conferring resistance to boll weevil and fall armyworm by usingmolecular tools. RESULTS: Four Brazilian cotton cultivars were microinjected with a minimal linear cassette generating 1248 putative lines. Complete gene integration was found in only one line (T0-34) containing one copy of cry1Ia detected by Southern blot. Protein was expressed in high concentration at 45 days after emergence (dae), decreasing by approximately 50% at 90 dae. Toxicity of the cry protein was demonstrated in feeding bioassays revealing 56.7% mortality to boll weevil fed buds and 88.1% mortality to fall armyworm fed leaves. A binding of cry1Ia antibody was found in the midgut of boll weevils fed on T0-34 buds in an immunodetection assay. CONCLUSION: The gene introduced into plants confers resistance to boll weevil and fall armyworm. Transmission of the transgene occurred normally to T1 progeny.All plants showed phenotypically normal growth,with fertile flowers and abundant seeds. 650 $acotton 650 $aAnthonomus grandis 650 $aGossypium hirsutum 650 $aSpodoptera frugiperda 653 $aChemical inseticides 700 1 $aMONNERAT, R. 700 1 $aLIMA, L. M. de 700 1 $aMARTINS, E. S. 700 1 $aMELO FILHO, P. A. 700 1 $aPINHEIRO, M. P. N. 700 1 $aSANTOS, R. C. 773 $tPest Management Science$gv. 72, p. 1549-1557, 2016.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registros recuperados : 38 | |
4. | | MELO FILHO, P. A.; DUSI, A. N.; BUSO, J. A.; TORRES, A. C.; RESENDE, R. O. Degenerescence of seed garlic after seven field exposures to natural virus infection. Virus: Reviews & Research, Florianópolis, v. 8, p. 184, set. 2003. Suplemento 1. Trabalho apresentado no 14. National Meeting of Virology, 2003, Florianópolis, SC, Brasil. Resumo.Biblioteca(s): Embrapa Hortaliças. |
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6. | | SILVA, F. A. C.; SILVA, C. R. C.; MELO FILHO, P. A.; SANTOS, R. C. dos. Expressão da clorofilase III em plantas de algodão resistente a ramulose. In.: WORKSHOP INTERNACIONAL EM BIOTECNOLOGIA, 1.; ENCONTRO ALFA-VALNATURA, 3.; JORNADA CIENTÍFICA DO LIKA, 3., 2008, Recife. Resumos...Recife: Ed. Universitária da UFPE, 2008. p. 387-388Tipo: Artigo em Anais de Congresso / Nota Técnica |
Biblioteca(s): Embrapa Algodão. |
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9. | | MELO FILHO, P. A.; DUSI, A. N.; BUSO, J. A.; TORRES, A. C.; RESENDE, R. O. Diversity of allexiviruses naturally infecting garlic fields in Brasil. Virus: Reviews & Research, Florianópolis, v. 8, p. 176, set. 2003. Suplemento 1. Trabalho apresentado no 14. National Meeting of Virology, 2003, Florianópolis, SC, Brasil. Resumo.Biblioteca(s): Embrapa Hortaliças. |
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11. | | SILVA, C. R. C.; SANTOS, R. C.; LUCENA, W. A.; LIMA, L. M.; MELO FILHO, P. A.; MONNERAT, R. Engenharia de piramidização de dois genes com propriedade inseticida para controle de pragas do algodão. In: ENCONTRO DE PRODUÇÃO CIENTÍFICA DA EMBRAPA ALGODÃO EPC 2007, 2., 2007. Campina Grande. Resumos...Campina Grande: Embrapa Algodão, 2007. p. 19 (Documentos, 187).Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Algodão. |
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13. | | ALVES, G. M. R.; PEREIRA, J. W. L.; LIMA, L. M. de; MELO FILHO, P. A.; SANTOS, R. C. dos. Componentes bioquímicos em linhagens intra-específicas de amendoim submetidas a estresse hídrico. CONGRESSO BRASILEIRO DE MAMONA, 5.; SIMPÓSIO INTERNACIONAL DE OLEAGINOSAS ENERGÉTICAS, 2.; FÓRUM CAPIXABA DE PINHÃO-MANSO, 1., 2012, Guarapari. Desafios e Oportunidades: anais. Campina Grande: Embrapa Algodão, 2012. p. 360Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Algodão. |
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19. | | SOARES, T. C.; CARVALHO, J. M. F. C.; LIMA, L. M. de; FREIRE, R. M. M.; SILVA, C. R. C.; MELO FILHO, P. A.; SANTOS, R. C. dos. Investigation on possible occurrence of somaclonal effects in zygotic and embryonic cotton structures based on agronomical traits. Genetics and Molecular Research, v. 16, n. 3, July 2017Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Algodão. |
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20. | | SILVA, F. A. C.; SANTOS, R. C. dos; AZEVEDO NETO, A.; GRANJA, M. M. C.; SOUZA, C. C. F. de; MELO FILHO, P. A. Descritores bioquímicos em cultivares de algodoeiro em resposta à inoculação com Colletotrichum gossypii var. cephalosporioides. Tropical plant pathology, v. 35, n. 2., p. 114-118, mar./apr., 2010.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
Biblioteca(s): Embrapa Algodão. |
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Registros recuperados : 38 | |
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