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Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
18/11/2019 |
Data da última atualização: |
28/11/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SANTOS, K. F. dos; ELOY, A. M. X.; MATOS, M. N. C.; PEIXOTO, R. M.; ARAGÃO, P. de T. T. D. de; PINHEIRO, R. R.; CUNHA, R. M. S. da. |
Afiliação: |
KATIANNE FREITAS DOS SANTOS, Universidade Estadual Vale do Acaraú (UVA) - Sobral, CE, Brazil; ANGELA MARIA XAVIER ELOY, CNPC; MARIA NÁGILA CARNEIRO MATOS, Universidade Federal do Ceará (UFC) - Fortaleza, CE, Brazil; RENATO MESQUITA PEIXOTO, Universidade Estadual do Ceará (UECE) - Fortaleza, CE, Brazil; PAULO DE TARSO TELES DOURADO DE ARAGÃO, Universidade Federal do Ceará (UFC) - Fortaleza, CE, Brazil; RAYMUNDO RIZALDO PINHEIRO, CNPC; RODRIGO MARANGUAPE SILVA DA CUNHA, Universidade Estadual Vale do Acaraú (UVA) - Sobral, CE, Brazil. |
Título: |
Use of proteomics in the study of the acute phase of caprine arthritis encephalitis in seminal plasma. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Small Ruminant Research, v. 181, p. 39-44, Dec. 2019. |
DOI: |
https://doi.org/10.1016/j.smallrumres.2019.10.004 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: Caprine arthritis encephalitis (CAE) is a chronic viral disease spread among dairy goats, caused by the virus of the Lentivirus genus, Orthoretrovirinae subfamily, and Retroviridae family, whose diagnostic tests are ineffective in view of the latency of the virus, making it difficult to control and treat it. As the acute phase of infection has not been studied yet and considering that the innate immune system participates in the initial direct line of defense, during which the virus is transmitted with very low efficiency, the aim of this work was to use proteomics tools to identify proteins and enzymes that may be present at this stage and may serve as indicators of infection. Seminal plasma of 05 Saanen animals was used, before and after infection with the CAE virus, in which two-dimensional electrophoresis was performed, followed by MS mass spectrometry. It was identified in some animals, at the first seroconversion by Western blotting (WB), positive significant expression of the Clusterin (score 789), Zinc-alpha2-glycoprotein (ZAG-2) (score 364), Prostaglandin? H2 D-isomerase precursor (score 85), and Serum Albumin (score 314), exception to Cystatin C (score 58). Taking into account that semen Clusterin in human binds with a very high affinity to DC-SIGN, an important piece in the recognition of pathogens by dendritic cells (DCs), canceling the binding of HIV-1 to DC-SIGN suggesting this protein may be an indicator of the CAEV infection. |
Palavras-Chave: |
Artrite Encefalite Caprina; CAE; CAEV; Caprino leiteiro. |
Thesagro: |
Doença Animal. |
Thesaurus Nal: |
Caprine arthritis-encephalitis; Dairy goats; Disease diagnosis; Goat diseases; Immunology; Proteomics. |
Categoria do assunto: |
H Saúde e Patologia |
Marc: |
LEADER 02497naa a2200337 a 4500 001 2114432 005 2019-11-28 008 2019 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.smallrumres.2019.10.004$2DOI 100 1 $aSANTOS, K. F. dos 245 $aUse of proteomics in the study of the acute phase of caprine arthritis encephalitis in seminal plasma.$h[electronic resource] 260 $c2019 520 $aAbstract: Caprine arthritis encephalitis (CAE) is a chronic viral disease spread among dairy goats, caused by the virus of the Lentivirus genus, Orthoretrovirinae subfamily, and Retroviridae family, whose diagnostic tests are ineffective in view of the latency of the virus, making it difficult to control and treat it. As the acute phase of infection has not been studied yet and considering that the innate immune system participates in the initial direct line of defense, during which the virus is transmitted with very low efficiency, the aim of this work was to use proteomics tools to identify proteins and enzymes that may be present at this stage and may serve as indicators of infection. Seminal plasma of 05 Saanen animals was used, before and after infection with the CAE virus, in which two-dimensional electrophoresis was performed, followed by MS mass spectrometry. It was identified in some animals, at the first seroconversion by Western blotting (WB), positive significant expression of the Clusterin (score 789), Zinc-alpha2-glycoprotein (ZAG-2) (score 364), Prostaglandin? H2 D-isomerase precursor (score 85), and Serum Albumin (score 314), exception to Cystatin C (score 58). Taking into account that semen Clusterin in human binds with a very high affinity to DC-SIGN, an important piece in the recognition of pathogens by dendritic cells (DCs), canceling the binding of HIV-1 to DC-SIGN suggesting this protein may be an indicator of the CAEV infection. 650 $aCaprine arthritis-encephalitis 650 $aDairy goats 650 $aDisease diagnosis 650 $aGoat diseases 650 $aImmunology 650 $aProteomics 650 $aDoença Animal 653 $aArtrite Encefalite Caprina 653 $aCAE 653 $aCAEV 653 $aCaprino leiteiro 700 1 $aELOY, A. M. X. 700 1 $aMATOS, M. N. C. 700 1 $aPEIXOTO, R. M. 700 1 $aARAGÃO, P. de T. T. D. de 700 1 $aPINHEIRO, R. R. 700 1 $aCUNHA, R. M. S. da 773 $tSmall Ruminant Research$gv. 181, p. 39-44, Dec. 2019.
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Embrapa Caprinos e Ovinos (CNPC) |
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Registros recuperados : 3 | |
1. | | SANTOS, K. F. dos; ELOY, A. M. X.; MATOS, M. N. C.; PEIXOTO, R. M.; ARAGÃO, P. de T. T. D. de; PINHEIRO, R. R.; CUNHA, R. M. S. da. Use of proteomics in the study of the acute phase of caprine arthritis encephalitis in seminal plasma. Small Ruminant Research, v. 181, p. 39-44, Dec. 2019.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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2. | | PINTO, T. M. F.; MOREIRA, R. F.; MATOS, M. N. C.; SOARES, V. V. M.; AGUIAR, M. V. de A.; ARAGÃO, P. de T. T. D. de; ALVES FILHO, J. G.; MORENO, F. B. M. B.; MONTEIRO-MOREIRA, A. C. de O.; COSTA, C. R. R.; LIMA FILHO, J. L. de; ELOY, A. M. X.; CUNHA, R. M. S. da. Evaluation of the proteomic profiles of ejaculated spermatozoa from Saanen bucks (Capra hircus). Animal Reproduction, v. 16, n. 4, p. 902-913, 2019.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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3. | | MOREIRA, R. F.; MATOS, M. N. C.; ALVES FILHO, J. G.; VALLE, R. V. do; ELOY, A. M. X.; PINTO, T. M. F.; MACHADO, S. P.; COSTA, C. R. R.; LIMA FILHO, J. L. de; LIMA, J. P. M. S.; CUNHA, R. M. S. da. Diversity of ejaculated sperm proteins in Moxotó bucks (Capra hircus) evaluated by multiple extraction methods. Animal Reproduction, Belo Horizonte, v. 15, n. 1, p. 84-92, jan./mar. 2018.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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Registros recuperados : 3 | |
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