02497naa a2200337 a 450000100080000000500110000800800410001902400590006010000220011924501300014126000090027152014800028065000350176065000160179565000220181165000180183365000150185165000150186665000190188165300310190065300080193165300090193965300210194870000190196970000200198870000190200870000310202770000200205870000230207877300580210121144322019-11-28       2019    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1016/j.smallrumres.2019.10.0042DOI1 aSANTOS, K. F. dos  aUse of proteomics in the study of the acute phase of caprine arthritis encephalitis in seminal plasma.h[electronic resource]  c2019  aAbstract: Caprine arthritis encephalitis (CAE) is a chronic viral disease spread among dairy goats, caused by the virus of the Lentivirus genus, Orthoretrovirinae subfamily, and Retroviridae family, whose diagnostic tests are ineffective in view of the latency of the virus, making it difficult to control and treat it. As the acute phase of infection has not been studied yet and considering that the innate immune system participates in the initial direct line of defense, during which the virus is transmitted with very low efficiency, the aim of this work was to use proteomics tools to identify proteins and enzymes that may be present at this stage and may serve as indicators of infection. Seminal plasma of 05 Saanen animals was used, before and after infection with the CAE virus, in which two-dimensional electrophoresis was performed, followed by MS mass spectrometry. It was identified in some animals, at the first seroconversion by Western blotting (WB), positive significant expression of the Clusterin (score 789), Zinc-alpha2-glycoprotein (ZAG-2) (score 364), Prostaglandin? H2 D-isomerase precursor (score 85), and Serum Albumin (score 314), exception to Cystatin C (score 58). Taking into account that semen Clusterin in human binds with a very high affinity to DC-SIGN, an important piece in the recognition of pathogens by dendritic cells (DCs), canceling the binding of HIV-1 to DC-SIGN suggesting this protein may be an indicator of the CAEV infection.  aCaprine arthritis-encephalitis  aDairy goats  aDisease diagnosis  aGoat diseases  aImmunology  aProteomics  aDoença Animal  aArtrite Encefalite Caprina  aCAE  aCAEV  aCaprino leiteiro1 aELOY, A. M. X.1 aMATOS, M. N. C.1 aPEIXOTO, R. M.1 aARAGÃO, P. de T. T. D. de1 aPINHEIRO, R. R.1 aCUNHA, R. M. S. da  tSmall Ruminant Researchgv. 181, p. 39-44, Dec. 2019.