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Registro Completo |
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Biblioteca(s): |
Embrapa Agropecuária Oeste; Embrapa Amazônia Ocidental; Embrapa Arroz e Feijão; Embrapa Clima Temperado; Embrapa Gado de Corte; Embrapa Mandioca e Fruticultura; Embrapa Meio Ambiente; Embrapa Meio-Norte; Embrapa Semiárido; Embrapa Soja; Embrapa Trigo. MenosEmbrapa Agropecuária Oeste; Embrapa Amazônia Ocidental; Embrapa Arroz e Feijão; Embrapa Clima Temperado; Embrapa Gado de Corte; Embrapa Mandioca e Fruticultura; Embrapa Meio Ambiente; Embrapa Meio-Norte; Embrapa Semiárido; Embrapa Soja... Mostrar Todas |
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Data corrente: |
29/03/1993 |
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Data da última atualização: |
03/02/2004 |
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Autoria: |
MASCARENHAS, H. A. A. |
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Título: |
Calcio, enxofre e ferro no solo e na planta. |
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Ano de publicação: |
1977 |
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Fonte/Imprenta: |
Campinas: Fundacao Cargill, 1977. |
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Páginas: |
95 p. |
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Idioma: |
Português |
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Conteúdo: |
Cálcio na planta. Absorção de cálcio pela raiz. Sinergismos e antagonismos. Cálcio no solo. Cálcio como fertilizante. O cálcio e o solo na planta. Enxofre no solo e na planta. O enxofre como nutriente. Fontes de adubos com enxofre. Atmosfera. Chuva. Disponibilidade no solo. Ciclo do enxofre. Imobilização. Oxidação de compostos inorgânicos. Redução de compostos inorgânicos de enxofre. Adsorção. Relações fisiológicas. Sintomatologia da deficiência. |
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Palavras-Chave: |
Fertiizante; Fertilizacao; Fertilization; Fertilizer; Macronutrientes; Mineral nutrition; Nuticao mineral; Soils Plant; Sulphur; Uso na agricultura. |
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Thesagro: |
Absorção; Adsorção; Adubação; Agricultura; Cálcio; Deficiência; Enxofre; Ferro; Fertilidade; Fertilizante; Mineral; Nutrição; Nutrição Vegetal; Planta; Solo. |
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Thesaurus Nal: |
absorption; adsorption; calcium; iron; Soil. |
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Categoria do assunto: |
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F Plantas e Produtos de Origem Vegetal
P Recursos Naturais, Ciências Ambientais e da Terra |
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Marc: |
LEADER 01594nam a2200481 a 4500
001 1211757
005 2004-02-03
008 1977 bl uuuu 00u1 u #d
100 1 $aMASCARENHAS, H. A. A.
245 $aCalcio, enxofre e ferro no solo e na planta.
260 $aCampinas: Fundacao Cargill$c1977
300 $a95 p.
520 $aCálcio na planta. Absorção de cálcio pela raiz. Sinergismos e antagonismos. Cálcio no solo. Cálcio como fertilizante. O cálcio e o solo na planta. Enxofre no solo e na planta. O enxofre como nutriente. Fontes de adubos com enxofre. Atmosfera. Chuva. Disponibilidade no solo. Ciclo do enxofre. Imobilização. Oxidação de compostos inorgânicos. Redução de compostos inorgânicos de enxofre. Adsorção. Relações fisiológicas. Sintomatologia da deficiência.
650 $aabsorption
650 $aadsorption
650 $acalcium
650 $airon
650 $aSoil
650 $aAbsorção
650 $aAdsorção
650 $aAdubação
650 $aAgricultura
650 $aCálcio
650 $aDeficiência
650 $aEnxofre
650 $aFerro
650 $aFertilidade
650 $aFertilizante
650 $aMineral
650 $aNutrição
650 $aNutrição Vegetal
650 $aPlanta
650 $aSolo
653 $aFertiizante
653 $aFertilizacao
653 $aFertilization
653 $aFertilizer
653 $aMacronutrientes
653 $aMineral nutrition
653 $aNuticao mineral
653 $aSoils Plant
653 $aSulphur
653 $aUso na agricultura
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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Data corrente: |
18/09/2020 |
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Data da última atualização: |
17/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MELO, B. P. de; LOURENCO-TESSUTTI, I. T.; MORGANTE, C. V.; SANTOS, N. C.; PINHEIRO, L. B.; LINS, C. B. de J.; SILVA, M. C. M.; MACEDO, L. L. P.; FONTES, E. P. B.; GROSSI-DE-SA, M. F. |
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Afiliação: |
BRUNO PAES DE MELO, UFV; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; CAROLINA VIANNA MORGANTE, CPATSA; NAIARA CORDEIRO SANTOS; LUANNA BEZERRA PINHEIRO, UCB; CAMILA BARROZO DE JESUS LINS; MARIA CRISTINA MATTAR DA SILVA, Cenargen; LEONARDO LIMA PEPINO DE MACEDO, Cenargen; ELIZABETH PACHECO BATISTA FONTES, UFV; MARIA FATIMA GROSSI DE SA, Cenargen. |
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Título: |
Soybean embryonic axis transformation: combining biolistic and Agrobacterium-Mediated Protocols to overcome typical complications of in vitro plant regeneration. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Frontiers in Plant Science, v. 11, article 1228, 2020. |
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DOI: |
https://doi.org/10.3389/fpls.2020.01228 |
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Idioma: |
Inglês |
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Conteúdo: |
The first successful attempt to generate genetically modified plants expressing a transgene was preformed via T-DNA-based gene transfer employing Agrobacterium tumefaciens-mediated genetic transformation. Limitations over infectivity and in vitro tissue culture led to the development of other DNA delivery systems, such as the biolistic method. Herein, we developed a new one-step protocol for transgenic soybean recovery by combining the two different transformation methods. This protocol comprises the following steps: agrobacterial preparation, seed sterilization, soybean embryo excision, shoot-cell injury by tungsten-microparticle bombardment, A. tumefaciens-mediated transformation, embryo co-cultivation in vitro, and selection of transgenic plants. This protocol can be completed in approximately 30?40 weeks. The average efficiency of producing transgenic soybean germlines using this protocol was 9.84%, similar to other previously described protocols. However, we introduced a more cost-effective, more straightforward and shorter methodology for transgenic plant recovery, which allows co-cultivation and plant regeneration in a single step, decreasing the chances of contamination and making the manipulation easier. Finally, as a hallmark, our protocol does not generate plant chimeras, in contrast to traditional plant regeneration protocols applied in other Agrobacterium-mediated transformation methods. Therefore, this new approach of plant transformation is applicable for studies of gene function and the production of transgenic cultivars carrying different traits for precision-breeding programs. MenosThe first successful attempt to generate genetically modified plants expressing a transgene was preformed via T-DNA-based gene transfer employing Agrobacterium tumefaciens-mediated genetic transformation. Limitations over infectivity and in vitro tissue culture led to the development of other DNA delivery systems, such as the biolistic method. Herein, we developed a new one-step protocol for transgenic soybean recovery by combining the two different transformation methods. This protocol comprises the following steps: agrobacterial preparation, seed sterilization, soybean embryo excision, shoot-cell injury by tungsten-microparticle bombardment, A. tumefaciens-mediated transformation, embryo co-cultivation in vitro, and selection of transgenic plants. This protocol can be completed in approximately 30?40 weeks. The average efficiency of producing transgenic soybean germlines using this protocol was 9.84%, similar to other previously described protocols. However, we introduced a more cost-effective, more straightforward and shorter methodology for transgenic plant recovery, which allows co-cultivation and plant regeneration in a single step, decreasing the chances of contamination and making the manipulation easier. Finally, as a hallmark, our protocol does not generate plant chimeras, in contrast to traditional plant regeneration protocols applied in other Agrobacterium-mediated transformation methods. Therefore, this new approach of plant transformation is applicable for stud... Mostrar Tudo |
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Palavras-Chave: |
Agrobacterium-mediated transformation; Embryonic axis; High-efficiency plant transformation; Particle bombardment; Planta geneticamente modificada; Recuperação trangênica de soja. |
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Thesagro: |
Cultura de Tecido; Glycine Max; Soja. |
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Thesaurus NAL: |
Agrobacterium; Embryonic structures; Genetic transformation; Plant genetics. |
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Categoria do assunto: |
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G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/216085/1/fpls-11-01228.pdf
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Marc: |
LEADER 02941naa a2200397 a 4500
001 2125013
005 2020-11-17
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3389/fpls.2020.01228$2DOI
100 1 $aMELO, B. P. de
245 $aSoybean embryonic axis transformation$bcombining biolistic and Agrobacterium-Mediated Protocols to overcome typical complications of in vitro plant regeneration.$h[electronic resource]
260 $c2020
520 $aThe first successful attempt to generate genetically modified plants expressing a transgene was preformed via T-DNA-based gene transfer employing Agrobacterium tumefaciens-mediated genetic transformation. Limitations over infectivity and in vitro tissue culture led to the development of other DNA delivery systems, such as the biolistic method. Herein, we developed a new one-step protocol for transgenic soybean recovery by combining the two different transformation methods. This protocol comprises the following steps: agrobacterial preparation, seed sterilization, soybean embryo excision, shoot-cell injury by tungsten-microparticle bombardment, A. tumefaciens-mediated transformation, embryo co-cultivation in vitro, and selection of transgenic plants. This protocol can be completed in approximately 30?40 weeks. The average efficiency of producing transgenic soybean germlines using this protocol was 9.84%, similar to other previously described protocols. However, we introduced a more cost-effective, more straightforward and shorter methodology for transgenic plant recovery, which allows co-cultivation and plant regeneration in a single step, decreasing the chances of contamination and making the manipulation easier. Finally, as a hallmark, our protocol does not generate plant chimeras, in contrast to traditional plant regeneration protocols applied in other Agrobacterium-mediated transformation methods. Therefore, this new approach of plant transformation is applicable for studies of gene function and the production of transgenic cultivars carrying different traits for precision-breeding programs.
650 $aAgrobacterium
650 $aEmbryonic structures
650 $aGenetic transformation
650 $aPlant genetics
650 $aCultura de Tecido
650 $aGlycine Max
650 $aSoja
653 $aAgrobacterium-mediated transformation
653 $aEmbryonic axis
653 $aHigh-efficiency plant transformation
653 $aParticle bombardment
653 $aPlanta geneticamente modificada
653 $aRecuperação trangênica de soja
700 1 $aLOURENCO-TESSUTTI, I. T.
700 1 $aMORGANTE, C. V.
700 1 $aSANTOS, N. C.
700 1 $aPINHEIRO, L. B.
700 1 $aLINS, C. B. de J.
700 1 $aSILVA, M. C. M.
700 1 $aMACEDO, L. L. P.
700 1 $aFONTES, E. P. B.
700 1 $aGROSSI-DE-SA, M. F.
773 $tFrontiers in Plant Science$gv. 11, article 1228, 2020.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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