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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
03/05/2010 |
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Data da última atualização: |
02/08/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
OCHOA-CORONA, F. M.; LOCALI-FABRIS, E. C.; FREITAS-ASTÚA, J. |
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Afiliação: |
Oklahoma University; Eliane Cristina Locali-Fabris, APTA; JULIANA DE FREITAS ASTUA, CNPMF. |
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Título: |
An RT-PCR procedure for detection and surveillance of Citrus leprosis virus C (CiLV-C) in post-entry quarantine stocks of citrus. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Phytopathology, St. Paul, v. 99, n. 6, S28, 2009. Suplement |
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Idioma: |
Inglês |
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Notas: |
Abstracts of presentations, Annual Meeting of the APS 2009. p. S28. |
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Conteúdo: |
Citrus leprosis virus C (CiLV-C), which is transmitted both mechanically and by the mite Brevipalpus phoenicis (Geijskes) (Acari: Tenuipalpidae), causes significant disease damage in South and Central America. Citrus leprosis disease was first recorded in Florida in 1925, but is believed eradicated through improved mite control procedures. CiLV-C is a threat to citrus producing nations where it is not present, such as New Zealand and the USA, and a sensitive detection method is required for screening and biosecurity of suspect quarantine material. CiLV-C is mechanically transmitted, posses a bipartite RNA genome and was believed to be a rhabdovirus. After being sequenced, CiLV-C was proposed as the type member of a new genus, Cilevirus, related to several (+) ssRNA viruses. Of two known morphological types of CiLV particles, the cytoplasmic type (CiLV-C) is prevalent than the nuclear type (CiLV-N) in Brazil and elsewhere. A pair of diagnostic primers, amplifying a segment of 278 bp located at the RNA-2 p15 gene of CiLV-C was designed using the Web software pathway Primer3-mFOLD-BLASTn. A thermodynamically robust RT-PCR that performs well in a range of melting temperatures and specifically optimized for CiLV-C was developed, and is a feasible tool to be used in quarantine. |
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Thesagro: |
Brevipalpus Phoenicis; Fruta Cítrica. |
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Thesaurus Nal: |
Citrus. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/748515/1/An-RT-PCR-procedure-for-detection-and-surveillance-of-Citrus-leprosis-virus-C-CiLV-C-in-post-entry-quarantine-stocks-of-citrus.pdf
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Marc: |
LEADER 01966nam a2200181 a 4500
001 1748515
005 2023-08-02
008 2009 bl uuuu u00u1 u #d
100 1 $aOCHOA-CORONA, F. M.
245 $aAn RT-PCR procedure for detection and surveillance of Citrus leprosis virus C (CiLV-C) in post-entry quarantine stocks of citrus.$h[electronic resource]
260 $aPhytopathology, St. Paul, v. 99, n. 6, S28, 2009. Suplement$c2009
500 $aAbstracts of presentations, Annual Meeting of the APS 2009. p. S28.
520 $aCitrus leprosis virus C (CiLV-C), which is transmitted both mechanically and by the mite Brevipalpus phoenicis (Geijskes) (Acari: Tenuipalpidae), causes significant disease damage in South and Central America. Citrus leprosis disease was first recorded in Florida in 1925, but is believed eradicated through improved mite control procedures. CiLV-C is a threat to citrus producing nations where it is not present, such as New Zealand and the USA, and a sensitive detection method is required for screening and biosecurity of suspect quarantine material. CiLV-C is mechanically transmitted, posses a bipartite RNA genome and was believed to be a rhabdovirus. After being sequenced, CiLV-C was proposed as the type member of a new genus, Cilevirus, related to several (+) ssRNA viruses. Of two known morphological types of CiLV particles, the cytoplasmic type (CiLV-C) is prevalent than the nuclear type (CiLV-N) in Brazil and elsewhere. A pair of diagnostic primers, amplifying a segment of 278 bp located at the RNA-2 p15 gene of CiLV-C was designed using the Web software pathway Primer3-mFOLD-BLASTn. A thermodynamically robust RT-PCR that performs well in a range of melting temperatures and specifically optimized for CiLV-C was developed, and is a feasible tool to be used in quarantine.
650 $aCitrus
650 $aBrevipalpus Phoenicis
650 $aFruta Cítrica
700 1 $aLOCALI-FABRIS, E. C.
700 1 $aFREITAS-ASTÚA, J.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Algodão. Para informações adicionais entre em contato com cnpa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Algodão. |
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Data corrente: |
01/10/2009 |
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Data da última atualização: |
18/03/2010 |
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Tipo da produção científica: |
Documentos |
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Autoria: |
LINS, O. B. D. S. M. D. O.; ACCIOLY, A. M. D. A.; BORGES, A. L.; MELO, C. A. S. de; JUNGHANS, D. T.; MEDEIROS, E. P. de; SOUZA, F. V. D.; LORDELO, I. da R.; SOUZA, L. D.; SOUZA, L. da S.; SANTANA, M. do A.; REBOUCAS, M. C. M.; FANCELLI, M.; COELHO FILHO, M. A.; CASTELLEN, M. da S.; LINS, O. B. dos S. M. de O.; PASSOS, O. S.; CORDEIRO, Z. J. M. |
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Afiliação: |
OLGA BENICIO DOS SANTOS M DE O LINS, CNPMF; ADRIANA MARIA DE AGUIAR ACCIOLY, CNPMF; ANA LUCIA BORGES, CNPMF; CARLOS ALBERTO SANTOS DE MELO, CNPMF; DAVI THEODORO JUNGHANS, CNPMF; EVERALDO PAULO DE MEDEIROS, CNPA; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; IARA DA ROCHA LORDELO, CNPMF; LAERCIO DUARTE SOUZA, CNPMF; LUCIANO DA SILVA SOUZA, CNPMF; MARCELO DO AMARAL SANTANA, CNPMF; MARIA CELESTE MARQUES REBOUCAS, CNPMF; MARILENE FANCELLI, CNPMF; MAURICIO ANTONIO COELHO FILHO, CNPMF; MILENE DA SILVA CASTELLEN, DPD; OLGA BENICIO DOS SANTOS M DE O LINS, CNPMF; ORLANDO SAMPAIO PASSOS, CNPMF; ZILTON JOSE MACIEL CORDEIRO, CNPMF. |
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Título: |
Diagnóstico rápido participativo do meio ambiente do Centro Nacional de Pesquisa de Mandioca e Fruticultura Tropical/Embrapa. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Cruz das Almas : Embrapa Mandioca e Fruticultura Tropical, 2009. |
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Páginas: |
40 p. |
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Descrição Física: |
il. |
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Série: |
(Documentos / Embrapa Mandioca e Fruticultura Tropical, 177). |
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ISSN: |
1516-5728 |
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Idioma: |
Português |
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Thesagro: |
Impacto Ambiental; Meio Ambiente. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01158nam a2200361 a 4500
001 1512977
005 2010-03-18
008 2009 bl uuuu u0uu1 u #d
022 $a1516-5728
100 1 $aLINS, O. B. D. S. M. D. O.
245 $aDiagnóstico rápido participativo do meio ambiente do Centro Nacional de Pesquisa de Mandioca e Fruticultura Tropical/Embrapa.
260 $aCruz das Almas : Embrapa Mandioca e Fruticultura Tropical$c2009
300 $a40 p.$cil.
490 $a(Documentos / Embrapa Mandioca e Fruticultura Tropical, 177).
650 $aImpacto Ambiental
650 $aMeio Ambiente
700 1 $aACCIOLY, A. M. D. A.
700 1 $aBORGES, A. L.
700 1 $aMELO, C. A. S. de
700 1 $aJUNGHANS, D. T.
700 1 $aMEDEIROS, E. P. de
700 1 $aSOUZA, F. V. D.
700 1 $aLORDELO, I. da R.
700 1 $aSOUZA, L. D.
700 1 $aSOUZA, L. da S.
700 1 $aSANTANA, M. do A.
700 1 $aREBOUCAS, M. C. M.
700 1 $aFANCELLI, M.
700 1 $aCOELHO FILHO, M. A.
700 1 $aCASTELLEN, M. da S.
700 1 $aLINS, O. B. dos S. M. de O.
700 1 $aPASSOS, O. S.
700 1 $aCORDEIRO, Z. J. M.
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Registro original: |
Embrapa Algodão (CNPA) |
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