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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia; Embrapa Semiárido. |
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Data corrente: |
22/02/2013 |
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Data da última atualização: |
09/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FERNANDES JUNIOR, P. I.; LIMA, A. A. de; PASSOS, S. R.; GAVA, C. A. T.; OLIVEIRA, P. J. de; RUMJANEK, N. G.; XAVIER, G. R. |
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Afiliação: |
PAULO IVAN FERNANDES JUNIOR, CPATSA; ANDRÉA APARECIDA DE LIMA, UFRRJ; SAMUEL RIBEIRO PASSOS, UFRRJ; CARLOS ALBERTO TUAO GAVA, CPATSA; PAULO JANSEN DE OLIVEIRA, UFRRJ; NORMA GOUVEA RUMJANEK, CNPAB; GUSTAVO RIBEIRO XAVIER, CNPAB. |
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Título: |
Phenotypic diversity and amylolytic activity of fast growing rhizobia from pigeonpea [Cajanus cajan (L.) Millsp.]. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, São Paulo, v. 43, n. 4, p. 1604-1612, 2012. |
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ISSN: |
1517-8382 |
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Idioma: |
Inglês |
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Conteúdo: |
This study evaluated 26 pigeonpea rhizobial isolates according to their cultural characteristics, intrinsic antibiotic resistance, salt and temperature tolerance, carbon source utilization and amylolytic activity. The cultural characterization showed that the majority of them presented the ability to acidify the YMA. Among the 27 isolates evaluated, 25 were able to grow when incubated at 42° C and 11 showed tolerance to 3% (w/v) of NaCl in YMA medium. The patterns of carbon sources utilization was very diverse among the isolates. It was observed the capacity of three strains to metabolize all the carbon sources evaluated and a total of 42% of the bacterial isolates was able to grow in the culture medium supplemented with at least, six carbon sources. The carbon sources mannitol (control) and sucrose were metabilized by all isolates evaluated. The profile of intrinsic resistance to antibiotics showed that the isolates were mostly resistant to streptomycin and ampicillin, but susceptible to kanamycin and chloranphenicol. High amylolytic activity of, at least, four isolates was also demonstrated, especially for isolated 47.3b, which showed the highest enzymatic index. These results indicate the metabolic versatility of the pigeonpea rhizobia, and indicates the isolate 47.3b to further studies regarding the amylase production and characterization |
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Palavras-Chave: |
Biological nitrogen fixation; Diversidade fenotípica; FBN; Fixação biológica de nitrogênio; Rizóbio; Seleção de estirpe; Seleção de estirpes; Strain selection. |
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Thesagro: |
Feijão; Feijão de Corda; Melhoramento Vegetal. |
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Thesaurus Nal: |
biotechnology; Molecular genetics; Nitrogen fixation; Pigeon peas. |
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Categoria do assunto: |
--
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/78769/1/Paulo-Ivan-2012.pdf
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Marc: |
LEADER 02518naa a2200385 a 4500
001 1950566
005 2023-01-09
008 2012 bl uuuu u00u1 u #d
022 $a1517-8382
100 1 $aFERNANDES JUNIOR, P. I.
245 $aPhenotypic diversity and amylolytic activity of fast growing rhizobia from pigeonpea [Cajanus cajan (L.) Millsp.].
260 $c2012
520 $aThis study evaluated 26 pigeonpea rhizobial isolates according to their cultural characteristics, intrinsic antibiotic resistance, salt and temperature tolerance, carbon source utilization and amylolytic activity. The cultural characterization showed that the majority of them presented the ability to acidify the YMA. Among the 27 isolates evaluated, 25 were able to grow when incubated at 42° C and 11 showed tolerance to 3% (w/v) of NaCl in YMA medium. The patterns of carbon sources utilization was very diverse among the isolates. It was observed the capacity of three strains to metabolize all the carbon sources evaluated and a total of 42% of the bacterial isolates was able to grow in the culture medium supplemented with at least, six carbon sources. The carbon sources mannitol (control) and sucrose were metabilized by all isolates evaluated. The profile of intrinsic resistance to antibiotics showed that the isolates were mostly resistant to streptomycin and ampicillin, but susceptible to kanamycin and chloranphenicol. High amylolytic activity of, at least, four isolates was also demonstrated, especially for isolated 47.3b, which showed the highest enzymatic index. These results indicate the metabolic versatility of the pigeonpea rhizobia, and indicates the isolate 47.3b to further studies regarding the amylase production and characterization
650 $abiotechnology
650 $aMolecular genetics
650 $aNitrogen fixation
650 $aPigeon peas
650 $aFeijão
650 $aFeijão de Corda
650 $aMelhoramento Vegetal
653 $aBiological nitrogen fixation
653 $aDiversidade fenotípica
653 $aFBN
653 $aFixação biológica de nitrogênio
653 $aRizóbio
653 $aSeleção de estirpe
653 $aSeleção de estirpes
653 $aStrain selection
700 1 $aLIMA, A. A. de
700 1 $aPASSOS, S. R.
700 1 $aGAVA, C. A. T.
700 1 $aOLIVEIRA, P. J. de
700 1 $aRUMJANEK, N. G.
700 1 $aXAVIER, G. R.
773 $tBrazilian Journal of Microbiology, São Paulo$gv. 43, n. 4, p. 1604-1612, 2012.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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