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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
29/09/2023 |
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Data da última atualização: |
29/09/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BRAGA, J. G. B.; NOVAIS, C. B. de; DINIZ, P. P.; ARAGÃO, O. O. da S.; SAGGIN JUNIOR, O. J.; JESUS, E. da C. |
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Afiliação: |
JOÃO GABRIEL BARBOSA BRAGA, UFLA; CÂNDIDO BARRETO DE NOVAIS, UFRRJ; PRISCILA PEREIRA DINIZ, UFLA; OSNAR OBEDE DA SILVA ARAGÃO, UFLA; ORIVALDO JOSE SAGGIN JUNIOR, CNPAB; EDERSON DA CONCEICAO JESUS, CNPAB. |
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Título: |
Association of mycoheterotrophic Gentianaceae with specific Glomus lineages. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Mycorrhiza, v. 33, n. 4, p. 249 - 256, July 2023. |
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Idioma: |
Inglês |
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Conteúdo: |
Some plant species took an alternative evolutionary pathway in which they lost their photosynthetic capacity to depend exclusively on carbon supplied by arbuscular mycorrhizal fungi (AMF) in an association called mycoheterotrophy. Among them is Voyriella parviflora, a species of the family Gentianaceae, which is found in tropical regions such as the Amazon basin. Here, we assessed the identity of AMF symbionts associated with this species. DNA was isolated from eight Gentianaceae specimens and from litter and surrounding roots of photosynthetic plants. The atp1 gene was amplified by Sanger sequencing to determine the taxonomic affiliation of the mycoheterotrophic plants. A 280 bp region of the 18S rRNA gene of AMF was amplified with primers NS31/AML2 by high-throughput sequencing. The mycoheterotrophic specimens were assigned to V. parviflora with a bootstrap support of 72%. Glomus was the most abundant AMF genus, both in the mycoheterotrophic plants and in the litter and roots of photosynthetic plants. In addition, a few Glomus genotypes were abundantly enriched in the mycoheterotrophic plants, with only a few specimens colonized by Gigaspora, Acaulospora, and Scutellospora in a low proportion. These genotypes formed a cluster within a larger clade, suggesting that V. parviflora shows a preferential association with a narrow Glomus lineage which is not phylogenetically close to a previously identified V. parviflora?s associated lineage. Furthermore, detecting fungi from other families suggests that V. parviflora is colonized by other genera, although with low frequency. These findings provide new insights into the association between AMF and mycoheterotrophic species and highlight the importance of considering trap culture-independent approaches in understanding this symbiosis MenosSome plant species took an alternative evolutionary pathway in which they lost their photosynthetic capacity to depend exclusively on carbon supplied by arbuscular mycorrhizal fungi (AMF) in an association called mycoheterotrophy. Among them is Voyriella parviflora, a species of the family Gentianaceae, which is found in tropical regions such as the Amazon basin. Here, we assessed the identity of AMF symbionts associated with this species. DNA was isolated from eight Gentianaceae specimens and from litter and surrounding roots of photosynthetic plants. The atp1 gene was amplified by Sanger sequencing to determine the taxonomic affiliation of the mycoheterotrophic plants. A 280 bp region of the 18S rRNA gene of AMF was amplified with primers NS31/AML2 by high-throughput sequencing. The mycoheterotrophic specimens were assigned to V. parviflora with a bootstrap support of 72%. Glomus was the most abundant AMF genus, both in the mycoheterotrophic plants and in the litter and roots of photosynthetic plants. In addition, a few Glomus genotypes were abundantly enriched in the mycoheterotrophic plants, with only a few specimens colonized by Gigaspora, Acaulospora, and Scutellospora in a low proportion. These genotypes formed a cluster within a larger clade, suggesting that V. parviflora shows a preferential association with a narrow Glomus lineage which is not phylogenetically close to a previously identified V. parviflora?s associated lineage. Furthermore, detecting fungi from oth... Mostrar Tudo |
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Palavras-Chave: |
Amazon; Metabarcoding; Mycoheterotrophy; Rbuscular mycorrhizal fungi. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02480naa a2200229 a 4500
001 2156980
005 2023-09-29
008 2023 bl uuuu u00u1 u #d
100 1 $aBRAGA, J. G. B.
245 $aAssociation of mycoheterotrophic Gentianaceae with specific Glomus lineages.$h[electronic resource]
260 $c2023
520 $aSome plant species took an alternative evolutionary pathway in which they lost their photosynthetic capacity to depend exclusively on carbon supplied by arbuscular mycorrhizal fungi (AMF) in an association called mycoheterotrophy. Among them is Voyriella parviflora, a species of the family Gentianaceae, which is found in tropical regions such as the Amazon basin. Here, we assessed the identity of AMF symbionts associated with this species. DNA was isolated from eight Gentianaceae specimens and from litter and surrounding roots of photosynthetic plants. The atp1 gene was amplified by Sanger sequencing to determine the taxonomic affiliation of the mycoheterotrophic plants. A 280 bp region of the 18S rRNA gene of AMF was amplified with primers NS31/AML2 by high-throughput sequencing. The mycoheterotrophic specimens were assigned to V. parviflora with a bootstrap support of 72%. Glomus was the most abundant AMF genus, both in the mycoheterotrophic plants and in the litter and roots of photosynthetic plants. In addition, a few Glomus genotypes were abundantly enriched in the mycoheterotrophic plants, with only a few specimens colonized by Gigaspora, Acaulospora, and Scutellospora in a low proportion. These genotypes formed a cluster within a larger clade, suggesting that V. parviflora shows a preferential association with a narrow Glomus lineage which is not phylogenetically close to a previously identified V. parviflora?s associated lineage. Furthermore, detecting fungi from other families suggests that V. parviflora is colonized by other genera, although with low frequency. These findings provide new insights into the association between AMF and mycoheterotrophic species and highlight the importance of considering trap culture-independent approaches in understanding this symbiosis
653 $aAmazon
653 $aMetabarcoding
653 $aMycoheterotrophy
653 $aRbuscular mycorrhizal fungi
700 1 $aNOVAIS, C. B. de
700 1 $aDINIZ, P. P.
700 1 $aARAGÃO, O. O. da S.
700 1 $aSAGGIN JUNIOR, O. J.
700 1 $aJESUS, E. da C.
773 $tMycorrhiza$gv. 33, n. 4, p. 249 - 256, July 2023.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Pesca e Aquicultura. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pesca e Aquicultura. |
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Data corrente: |
02/06/2022 |
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Data da última atualização: |
02/06/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
RAMOS, A. M. J.; RODRIGUES, A. P. O.; FREITAS, L. E. L. de; SANTOS, V. R. V. dos; MATTIONI, B.; FRACALOSSI, D. M. |
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Afiliação: |
ANTÔNIO MARCOS JAQUES RAMOS, UFSC; ANA PAULA OEDA RODRIGUES, CNPASA; LUIZ EDUARDO LIMA DE FREITAS, CNPASA; VIVIANE RODRIGUES V DOS SANTOS, CNPASA; BRUNA MATTIONI, KANSAS STATE UNIVERSITY; DÉBORA MACHADO FRACALOSSI, UFSC. |
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Título: |
Starchy plant ingredients in pirarucu (Arapaima gigas) feeds: utilization potential based on apparent digestibility and starch microstructure. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Aquaculture Research, 2022. |
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DOI: |
https://doi.org/10.1111/are.15914 |
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Idioma: |
Inglês |
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Conteúdo: |
Pirarucu (Arapaima gigas) is an Amazonian carnivorous fish species with great aquaculture potential. The apparent digestibility coefficients (ADC) of corn, low-tannin sorghum, broken rice, defatted rice bran, and wheat bran were determined for juvenile pirarucu using a completely randomized block design (two time blocks with two replications per block). We also evaluated the influence of digestion on the structure of starch granules by scanning electron microscopy (SEM) of the feed and faeces. The ADC of nutrients and energy, except protein, differed among plant sources. Broken rice and corn were the most digestible starch sources for dry matter (81.14 +- 8201;1.52) and energy (77.66 +-2.36) respectively. Starch digestibility was inversely related to the starch content and directly related to the fibre content of the tested ingredients. The highest and lowest starch ADC were detected for wheat bran (103.44 +- 5.41) and low-tannin sorghum (53.35 +- 5.18). Structures similar to spherulites were detected in the faecal samples of corn-fed fish, possibly owing to lower starch digestibility. Considering the energetic purpose of the tested ingredients, corn and broken rice were the most suitable energetic sources because they provided higher digestible starch and energy values. |
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Thesagro: |
Nutrição Animal; Peixe; Pirarucu. |
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Thesaurus NAL: |
Arapaima gigas; Digestibility; Fish; Microstructure; Proteins; Starch granules; Starch products. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02220naa a2200313 a 4500
001 2143635
005 2022-06-02
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1111/are.15914$2DOI
100 1 $aRAMOS, A. M. J.
245 $aStarchy plant ingredients in pirarucu (Arapaima gigas) feeds$butilization potential based on apparent digestibility and starch microstructure.$h[electronic resource]
260 $c2022
520 $aPirarucu (Arapaima gigas) is an Amazonian carnivorous fish species with great aquaculture potential. The apparent digestibility coefficients (ADC) of corn, low-tannin sorghum, broken rice, defatted rice bran, and wheat bran were determined for juvenile pirarucu using a completely randomized block design (two time blocks with two replications per block). We also evaluated the influence of digestion on the structure of starch granules by scanning electron microscopy (SEM) of the feed and faeces. The ADC of nutrients and energy, except protein, differed among plant sources. Broken rice and corn were the most digestible starch sources for dry matter (81.14 +- 8201;1.52) and energy (77.66 +-2.36) respectively. Starch digestibility was inversely related to the starch content and directly related to the fibre content of the tested ingredients. The highest and lowest starch ADC were detected for wheat bran (103.44 +- 5.41) and low-tannin sorghum (53.35 +- 5.18). Structures similar to spherulites were detected in the faecal samples of corn-fed fish, possibly owing to lower starch digestibility. Considering the energetic purpose of the tested ingredients, corn and broken rice were the most suitable energetic sources because they provided higher digestible starch and energy values.
650 $aArapaima gigas
650 $aDigestibility
650 $aFish
650 $aMicrostructure
650 $aProteins
650 $aStarch granules
650 $aStarch products
650 $aNutrição Animal
650 $aPeixe
650 $aPirarucu
700 1 $aRODRIGUES, A. P. O.
700 1 $aFREITAS, L. E. L. de
700 1 $aSANTOS, V. R. V. dos
700 1 $aMATTIONI, B.
700 1 $aFRACALOSSI, D. M.
773 $tAquaculture Research, 2022.
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Embrapa Pesca e Aquicultura (CNPASA) |
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