02480naa a2200229 a 450000100080000000500110000800800410001910000200006024501040008026000090018452018130019365300110200665300180201765300210203565300320205670000210208870000170210970000250212670000250215170000200217677300540219621569802023-09-29       2023    bl uuuu     u00u1 u    #d1 aBRAGA, J. G. B.  aAssociation of mycoheterotrophic Gentianaceae with specific Glomus lineages.h[electronic resource]  c2023  aSome plant species took an alternative evolutionary pathway in which they lost their photosynthetic capacity to depend exclusively on carbon supplied by arbuscular mycorrhizal fungi (AMF) in an association called mycoheterotrophy. Among them is Voyriella parviflora, a species of the family Gentianaceae, which is found in tropical regions such as the Amazon basin. Here, we assessed the identity of AMF symbionts associated with this species. DNA was isolated from eight Gentianaceae specimens and from litter and surrounding roots of photosynthetic plants. The atp1 gene was amplified by Sanger sequencing to determine the taxonomic affiliation of the mycoheterotrophic plants. A 280 bp region of the 18S rRNA gene of AMF was amplified with primers NS31/AML2 by high-throughput sequencing. The mycoheterotrophic specimens were assigned to V. parviflora with a bootstrap support of 72%. Glomus was the most abundant AMF genus, both in the mycoheterotrophic plants and in the litter and roots of photosynthetic plants. In addition, a few Glomus genotypes were abundantly enriched in the mycoheterotrophic plants, with only a few specimens colonized by Gigaspora, Acaulospora, and Scutellospora in a low proportion. These genotypes formed a cluster within a larger clade, suggesting that V. parviflora shows a preferential association with a narrow Glomus lineage which is not phylogenetically close to a previously identified V. parviflora?s associated lineage. Furthermore, detecting fungi from other families suggests that V. parviflora is colonized by other genera, although with low frequency. These findings provide new insights into the association between AMF and mycoheterotrophic species and highlight the importance of considering trap culture-independent approaches in understanding this symbiosis  aAmazon  aMetabarcoding  aMycoheterotrophy  aRbuscular mycorrhizal fungi1 aNOVAIS, C. B. de1 aDINIZ, P. P.1 aARAGÃO, O. O. da S.1 aSAGGIN JUNIOR, O. J.1 aJESUS, E. da C.  tMycorrhizagv. 33, n. 4, p. 249 - 256, July 2023.