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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
30/12/2019 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PILLAI, V. V.; SIQUEIRA, L. G. B.; DAS, M.; KEI, T. G.; TU, L. N.; HERREN, A. W.; PHINNEY, B. S.; CHEONG, S. H.; HANSEN, P. J.; SELVARAJ, V. |
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Afiliação: |
VIJU VIJAYAN PILLAI, Cornell University, Ithaca, NY; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; MOUBANI DAS, Cornell University, Ithaca, NY; TIFFANY G. KEI, Cornell University, Ithaca, NY; LAN N. TU, Cornell University, Ithaca, NY; ANTHONY W. HERREN, University of California; BRETT S. PHINNEY, University of California; SOON HON CHEONG, Cornell University, Ithaca, NY; PETER J. HANSEN, University of Florida, Gainesville, FL; VIMAL SELVARAJ, Cornell University, Ithaca, NY. |
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Título: |
Physiological profile of undifferentiated bovine blastocyst-derived trophoblasts. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Biology Open, v. 8, n. 5, article bio037937, 2019. |
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DOI: |
https://doi.org/10.1242/bio.037937 |
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Idioma: |
Inglês |
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Conteúdo: |
Trophectoderm of blastocysts mediate early events in fetal-maternal communication, enabling implantation and establishment of a functional placenta. Inadequate or impaired developmental events linked to trophoblasts directly impact early embryo survival and successful implantation during a crucial period that corresponds with high incidence of pregnancy losses in dairy cows. As yet, the molecular basis of bovine trophectoderm development and signaling towards initiation of implantation remains poorly understood. In this study, we developed methods for culturing undifferentiated bovine blastocyst-derived trophoblasts and used both transcriptomics and proteomics in early colonies to categorize and elucidate their functional characteristics. A total of 9270 transcripts and 1418 proteins were identified and analyzed based on absolute abundance. We profiled an extensive list of growth factors, cytokines and other relevant factors that can effectively influence paracrine communication in the uterine microenvironment. Functional categorization and analysis revealed novel information on structural organization, extracellular matrix composition, cell junction and adhesion components, transcription networks, and metabolic preferences. Our data showcase the fundamental physiology of bovine trophectoderm and indicate hallmarks of the self-renewing undifferentiated state akin to trophoblast stem cells described in other species. Functional features uncovered are essential for understanding early events in bovine pregnancy towards initiation of implantation. MenosTrophectoderm of blastocysts mediate early events in fetal-maternal communication, enabling implantation and establishment of a functional placenta. Inadequate or impaired developmental events linked to trophoblasts directly impact early embryo survival and successful implantation during a crucial period that corresponds with high incidence of pregnancy losses in dairy cows. As yet, the molecular basis of bovine trophectoderm development and signaling towards initiation of implantation remains poorly understood. In this study, we developed methods for culturing undifferentiated bovine blastocyst-derived trophoblasts and used both transcriptomics and proteomics in early colonies to categorize and elucidate their functional characteristics. A total of 9270 transcripts and 1418 proteins were identified and analyzed based on absolute abundance. We profiled an extensive list of growth factors, cytokines and other relevant factors that can effectively influence paracrine communication in the uterine microenvironment. Functional categorization and analysis revealed novel information on structural organization, extracellular matrix composition, cell junction and adhesion components, transcription networks, and metabolic preferences. Our data showcase the fundamental physiology of bovine trophectoderm and indicate hallmarks of the self-renewing undifferentiated state akin to trophoblast stem cells described in other species. Functional features uncovered are essential for understandi... Mostrar Tudo |
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Thesagro: |
Placenta. |
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Thesaurus Nal: |
Blastocyst; Embryo implantation; Pregnancy; Stem cells; Trophoblast. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/207915/1/BiolOpen-LGustavoSiq-Physiological-bio037937.full.pdf
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Marc: |
LEADER 02435naa a2200313 a 4500
001 2117829
005 2024-02-06
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1242/bio.037937$2DOI
100 1 $aPILLAI, V. V.
245 $aPhysiological profile of undifferentiated bovine blastocyst-derived trophoblasts.$h[electronic resource]
260 $c2019
520 $aTrophectoderm of blastocysts mediate early events in fetal-maternal communication, enabling implantation and establishment of a functional placenta. Inadequate or impaired developmental events linked to trophoblasts directly impact early embryo survival and successful implantation during a crucial period that corresponds with high incidence of pregnancy losses in dairy cows. As yet, the molecular basis of bovine trophectoderm development and signaling towards initiation of implantation remains poorly understood. In this study, we developed methods for culturing undifferentiated bovine blastocyst-derived trophoblasts and used both transcriptomics and proteomics in early colonies to categorize and elucidate their functional characteristics. A total of 9270 transcripts and 1418 proteins were identified and analyzed based on absolute abundance. We profiled an extensive list of growth factors, cytokines and other relevant factors that can effectively influence paracrine communication in the uterine microenvironment. Functional categorization and analysis revealed novel information on structural organization, extracellular matrix composition, cell junction and adhesion components, transcription networks, and metabolic preferences. Our data showcase the fundamental physiology of bovine trophectoderm and indicate hallmarks of the self-renewing undifferentiated state akin to trophoblast stem cells described in other species. Functional features uncovered are essential for understanding early events in bovine pregnancy towards initiation of implantation.
650 $aBlastocyst
650 $aEmbryo implantation
650 $aPregnancy
650 $aStem cells
650 $aTrophoblast
650 $aPlacenta
700 1 $aSIQUEIRA, L. G. B.
700 1 $aDAS, M.
700 1 $aKEI, T. G.
700 1 $aTU, L. N.
700 1 $aHERREN, A. W.
700 1 $aPHINNEY, B. S.
700 1 $aCHEONG, S. H.
700 1 $aHANSEN, P. J.
700 1 $aSELVARAJ, V.
773 $tBiology Open$gv. 8, n. 5, article bio037937, 2019.
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Embrapa Gado de Leite (CNPGL) |
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| 14. |  | BELLAVER, C.; FIALHO, E. T.; PROTAS, J. F.; GRUMANN, A. Cama de aviário em rações para suínos em terminação: desempenho, digestibilidade e economia. Pesquisa Agropecuária Brasileira, Brasília, v.18, n.7, p. 797-804, jul. 1983. Título em inglês: Effects of feeding poutry beds on the performance, digestibility and economics in finishing pigs.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 20. | | FIALHO, E. T.; SOBESTIANSKY, J.; WENTZ, IVO; BELLAVER, C. Levantamento de composicao quimica de racoes, concentrados e ingredientes utilizados em granjas de reprodutores, em Santa Catarina. In: CONGRESSO BRASILEIRO DE MEDICINA VETERINARIA, 18., 1982, Balneario Camboriu, SC. Resumos... Florianopolis: SBMV / SOMEVESC, 1982. p.333 CONGRESSO INTERNACIONAL DE VETERINARIA EM LINGUA PORTUGUESA, 3.; SEMINARIO BRASILEIRO DE PARASITOLOGIA VETERINARIA, 3., 1982, Balneario Camboriu, SC.| Biblioteca(s): Embrapa Suínos e Aves. |
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