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Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
03/05/2010 |
Data da última atualização: |
02/08/2023 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
OCHOA-CORONA, F. M.; LOCALI-FABRIS, E. C.; FREITAS-ASTÚA, J. |
Afiliação: |
Oklahoma University; Eliane Cristina Locali-Fabris, APTA; JULIANA DE FREITAS ASTUA, CNPMF. |
Título: |
An RT-PCR procedure for detection and surveillance of Citrus leprosis virus C (CiLV-C) in post-entry quarantine stocks of citrus. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
Phytopathology, St. Paul, v. 99, n. 6, S28, 2009. Suplement |
Idioma: |
Inglês |
Notas: |
Abstracts of presentations, Annual Meeting of the APS 2009. p. S28. |
Conteúdo: |
Citrus leprosis virus C (CiLV-C), which is transmitted both mechanically and by the mite Brevipalpus phoenicis (Geijskes) (Acari: Tenuipalpidae), causes significant disease damage in South and Central America. Citrus leprosis disease was first recorded in Florida in 1925, but is believed eradicated through improved mite control procedures. CiLV-C is a threat to citrus producing nations where it is not present, such as New Zealand and the USA, and a sensitive detection method is required for screening and biosecurity of suspect quarantine material. CiLV-C is mechanically transmitted, posses a bipartite RNA genome and was believed to be a rhabdovirus. After being sequenced, CiLV-C was proposed as the type member of a new genus, Cilevirus, related to several (+) ssRNA viruses. Of two known morphological types of CiLV particles, the cytoplasmic type (CiLV-C) is prevalent than the nuclear type (CiLV-N) in Brazil and elsewhere. A pair of diagnostic primers, amplifying a segment of 278 bp located at the RNA-2 p15 gene of CiLV-C was designed using the Web software pathway Primer3-mFOLD-BLASTn. A thermodynamically robust RT-PCR that performs well in a range of melting temperatures and specifically optimized for CiLV-C was developed, and is a feasible tool to be used in quarantine. |
Thesagro: |
Brevipalpus Phoenicis; Fruta Cítrica. |
Thesaurus Nal: |
Citrus. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/748515/1/An-RT-PCR-procedure-for-detection-and-surveillance-of-Citrus-leprosis-virus-C-CiLV-C-in-post-entry-quarantine-stocks-of-citrus.pdf
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Marc: |
LEADER 01966nam a2200181 a 4500 001 1748515 005 2023-08-02 008 2009 bl uuuu u00u1 u #d 100 1 $aOCHOA-CORONA, F. M. 245 $aAn RT-PCR procedure for detection and surveillance of Citrus leprosis virus C (CiLV-C) in post-entry quarantine stocks of citrus.$h[electronic resource] 260 $aPhytopathology, St. Paul, v. 99, n. 6, S28, 2009. Suplement$c2009 500 $aAbstracts of presentations, Annual Meeting of the APS 2009. p. S28. 520 $aCitrus leprosis virus C (CiLV-C), which is transmitted both mechanically and by the mite Brevipalpus phoenicis (Geijskes) (Acari: Tenuipalpidae), causes significant disease damage in South and Central America. Citrus leprosis disease was first recorded in Florida in 1925, but is believed eradicated through improved mite control procedures. CiLV-C is a threat to citrus producing nations where it is not present, such as New Zealand and the USA, and a sensitive detection method is required for screening and biosecurity of suspect quarantine material. CiLV-C is mechanically transmitted, posses a bipartite RNA genome and was believed to be a rhabdovirus. After being sequenced, CiLV-C was proposed as the type member of a new genus, Cilevirus, related to several (+) ssRNA viruses. Of two known morphological types of CiLV particles, the cytoplasmic type (CiLV-C) is prevalent than the nuclear type (CiLV-N) in Brazil and elsewhere. A pair of diagnostic primers, amplifying a segment of 278 bp located at the RNA-2 p15 gene of CiLV-C was designed using the Web software pathway Primer3-mFOLD-BLASTn. A thermodynamically robust RT-PCR that performs well in a range of melting temperatures and specifically optimized for CiLV-C was developed, and is a feasible tool to be used in quarantine. 650 $aCitrus 650 $aBrevipalpus Phoenicis 650 $aFruta Cítrica 700 1 $aLOCALI-FABRIS, E. C. 700 1 $aFREITAS-ASTÚA, J.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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Registros recuperados : 111 | |
3. | | KUBO, K. S.; FREITAS-ÁSTUA, J.; KITAGIMA, E. W. Detecção do Clerodendron chlorotic sport virus in planta e no ácaro vetor. Summa Phytopathologica, Botucatu, v. 34, , p. 97, fev. 2008. Suplemento. Edição dos Resumos do XXXI Congresso Paulista de Fitopatologia, Campinas, fev. 2008.
R 301Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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5. | | FREITAS-ÁSTUA, J.; NICOLINI, F.; BASTIANEL, M.; KITAJIMA, E. W. Interações entre o vírus da leprose dos citros e seu vetor Brevipalpus phoenicis. Summa Phytopathologica, Botucatu, v. 34, , p. 128, fev. 2008. Suplemento. Edição dos Resumos do XXXI Congresso Paulista de Fitopatologia, Campinas, fev. 2008.Tipo: Artigo em Anais de Congresso / Nota Técnica |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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7. | | BASTIANEL, M.; NOVELLI, V. M.; FREITAS-ASTUA, J. de. Field assessments reveal different levels of susceptibility among mandarins and hybrids to Citrus Leprosis Virus c. In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. 129.PS2
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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8. | | MASCARENHAS, G.; BARBOSA, C. de J.; KITAJIMA, E. W.; FREITAS-ÁSTUA, J. Alta eficiência de transmissão mecânica e por vetor do vírus da pinta verde (PFGSV) para maracujazeiro. Tropical Plant Pathology, Brasília, DF, v. 34, ago. 2009. Suplemento. Edição dos Resumos do XLII Congresso Brasileiro de Fitopatologia, Rio de Janeiro, ago. 2009. Suplemento. Resumo 911.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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9. | | RODRIGUES, A. S.; BARBOSA, C. de J.; SOARES FILHO, W. dos S.; FREITAS-ASTUA, J. de. Citrus rootstock hybrids tolerants to citrus tristeza virus and Bahia bark scaling disease. In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. 106_PS2
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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10. | | KITAJIMA, E. W.; CHAGAS, C. M.; HARAKAWA, R.; FREITAS-ASTÚA, J.; RODRIGUES, J. C. V. Citrus leprosis in Florida may have been of the nuclear type. Virus Reviews & Research, Rio de Janeiro, v. 14, p. 90, 2009. Suplemento 1. Trabalho apresentado no XX Encontro Nacional de Virologia, Brasília, DF. Resumo 098.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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11. | | TASSI, A. D.; FREITAS-ASTÚA, J.; JADÃO, A.; KITAJIMA, E. W. Citrus leprosis virus (CILV-C) vector relationship assessed by bean as test plant. Virus Reviews & Research, Rio de Janeiro, v. 14, p. 90, 2009. Suplplement 1. Trabalho apresentado no XX Encontro Nacional de Virologia, Brasília, DF. Resumo 097Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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14. | | BASTIANEL, M.; FREITAS-ÁSTUA, J.; NICOLINI, F.; CRISTOFAI-YALY, M.; NOVELLI, V. M.; MACHADO, M. A. Differential gene response of citrus to CiLV. In: INTERNATIONAL CITRUS CONGRESS, 11., 2008, Wuhan, China. Program and abstracts... Wuhan: The International Society of Citriculture, 2008. p. 64. 115Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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15. | | ANTONIOLI-LUIZON, R.; FREITAS-ÁSTUA, J.; REZENDE, J. A. M.; MACHADO, M. A.; KITAJIMA, E. W. Detecção específica do vírus da pinta verde do maracujazeiro por RT-PCR. Tropical Plant Pathology, Brasília, DF, v. 34, ago. 2009. Suplemento. Edição dos Resumos do XLII Congresso Brasileiro de Fitopatologia, Rio de Janeiro, ago. 2009. Suplemento. Resumo 907.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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20. | | FREITAS-ÁSTUA, J.; BASTIANEL, M.; NICOLINI, F.; SCHONS, J.; KITAJIMA, E. W.; MACHADO, M. A. Evidences to support that Citrus leprosis virus and its mite vector interact in a circulative - but not propagative - manner. In: INTERNATIONAL CITRUS CONGRESS, 11., 2008, Wuhan, China. Program and abstracts... Wuhan: The International Society of Citriculture, 2008. p. 237-238. P349Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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Registros recuperados : 111 | |
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