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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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Data corrente: |
18/09/2020 |
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Data da última atualização: |
17/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MELO, B. P. de; LOURENCO-TESSUTTI, I. T.; MORGANTE, C. V.; SANTOS, N. C.; PINHEIRO, L. B.; LINS, C. B. de J.; SILVA, M. C. M.; MACEDO, L. L. P.; FONTES, E. P. B.; GROSSI-DE-SA, M. F. |
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Afiliação: |
BRUNO PAES DE MELO, UFV; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; CAROLINA VIANNA MORGANTE, CPATSA; NAIARA CORDEIRO SANTOS; LUANNA BEZERRA PINHEIRO, UCB; CAMILA BARROZO DE JESUS LINS; MARIA CRISTINA MATTAR DA SILVA, Cenargen; LEONARDO LIMA PEPINO DE MACEDO, Cenargen; ELIZABETH PACHECO BATISTA FONTES, UFV; MARIA FATIMA GROSSI DE SA, Cenargen. |
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Título: |
Soybean embryonic axis transformation: combining biolistic and Agrobacterium-Mediated Protocols to overcome typical complications of in vitro plant regeneration. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Frontiers in Plant Science, v. 11, article 1228, 2020. |
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DOI: |
https://doi.org/10.3389/fpls.2020.01228 |
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Idioma: |
Inglês |
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Conteúdo: |
The first successful attempt to generate genetically modified plants expressing a transgene was preformed via T-DNA-based gene transfer employing Agrobacterium tumefaciens-mediated genetic transformation. Limitations over infectivity and in vitro tissue culture led to the development of other DNA delivery systems, such as the biolistic method. Herein, we developed a new one-step protocol for transgenic soybean recovery by combining the two different transformation methods. This protocol comprises the following steps: agrobacterial preparation, seed sterilization, soybean embryo excision, shoot-cell injury by tungsten-microparticle bombardment, A. tumefaciens-mediated transformation, embryo co-cultivation in vitro, and selection of transgenic plants. This protocol can be completed in approximately 30?40 weeks. The average efficiency of producing transgenic soybean germlines using this protocol was 9.84%, similar to other previously described protocols. However, we introduced a more cost-effective, more straightforward and shorter methodology for transgenic plant recovery, which allows co-cultivation and plant regeneration in a single step, decreasing the chances of contamination and making the manipulation easier. Finally, as a hallmark, our protocol does not generate plant chimeras, in contrast to traditional plant regeneration protocols applied in other Agrobacterium-mediated transformation methods. Therefore, this new approach of plant transformation is applicable for studies of gene function and the production of transgenic cultivars carrying different traits for precision-breeding programs. MenosThe first successful attempt to generate genetically modified plants expressing a transgene was preformed via T-DNA-based gene transfer employing Agrobacterium tumefaciens-mediated genetic transformation. Limitations over infectivity and in vitro tissue culture led to the development of other DNA delivery systems, such as the biolistic method. Herein, we developed a new one-step protocol for transgenic soybean recovery by combining the two different transformation methods. This protocol comprises the following steps: agrobacterial preparation, seed sterilization, soybean embryo excision, shoot-cell injury by tungsten-microparticle bombardment, A. tumefaciens-mediated transformation, embryo co-cultivation in vitro, and selection of transgenic plants. This protocol can be completed in approximately 30?40 weeks. The average efficiency of producing transgenic soybean germlines using this protocol was 9.84%, similar to other previously described protocols. However, we introduced a more cost-effective, more straightforward and shorter methodology for transgenic plant recovery, which allows co-cultivation and plant regeneration in a single step, decreasing the chances of contamination and making the manipulation easier. Finally, as a hallmark, our protocol does not generate plant chimeras, in contrast to traditional plant regeneration protocols applied in other Agrobacterium-mediated transformation methods. Therefore, this new approach of plant transformation is applicable for stud... Mostrar Tudo |
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Palavras-Chave: |
Agrobacterium-mediated transformation; Embryonic axis; High-efficiency plant transformation; Particle bombardment; Planta geneticamente modificada; Recuperação trangênica de soja. |
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Thesagro: |
Cultura de Tecido; Glycine Max; Soja. |
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Thesaurus Nal: |
Agrobacterium; Embryonic structures; Genetic transformation; Plant genetics. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/216085/1/fpls-11-01228.pdf
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Marc: |
LEADER 02941naa a2200397 a 4500
001 2125013
005 2020-11-17
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3389/fpls.2020.01228$2DOI
100 1 $aMELO, B. P. de
245 $aSoybean embryonic axis transformation$bcombining biolistic and Agrobacterium-Mediated Protocols to overcome typical complications of in vitro plant regeneration.$h[electronic resource]
260 $c2020
520 $aThe first successful attempt to generate genetically modified plants expressing a transgene was preformed via T-DNA-based gene transfer employing Agrobacterium tumefaciens-mediated genetic transformation. Limitations over infectivity and in vitro tissue culture led to the development of other DNA delivery systems, such as the biolistic method. Herein, we developed a new one-step protocol for transgenic soybean recovery by combining the two different transformation methods. This protocol comprises the following steps: agrobacterial preparation, seed sterilization, soybean embryo excision, shoot-cell injury by tungsten-microparticle bombardment, A. tumefaciens-mediated transformation, embryo co-cultivation in vitro, and selection of transgenic plants. This protocol can be completed in approximately 30?40 weeks. The average efficiency of producing transgenic soybean germlines using this protocol was 9.84%, similar to other previously described protocols. However, we introduced a more cost-effective, more straightforward and shorter methodology for transgenic plant recovery, which allows co-cultivation and plant regeneration in a single step, decreasing the chances of contamination and making the manipulation easier. Finally, as a hallmark, our protocol does not generate plant chimeras, in contrast to traditional plant regeneration protocols applied in other Agrobacterium-mediated transformation methods. Therefore, this new approach of plant transformation is applicable for studies of gene function and the production of transgenic cultivars carrying different traits for precision-breeding programs.
650 $aAgrobacterium
650 $aEmbryonic structures
650 $aGenetic transformation
650 $aPlant genetics
650 $aCultura de Tecido
650 $aGlycine Max
650 $aSoja
653 $aAgrobacterium-mediated transformation
653 $aEmbryonic axis
653 $aHigh-efficiency plant transformation
653 $aParticle bombardment
653 $aPlanta geneticamente modificada
653 $aRecuperação trangênica de soja
700 1 $aLOURENCO-TESSUTTI, I. T.
700 1 $aMORGANTE, C. V.
700 1 $aSANTOS, N. C.
700 1 $aPINHEIRO, L. B.
700 1 $aLINS, C. B. de J.
700 1 $aSILVA, M. C. M.
700 1 $aMACEDO, L. L. P.
700 1 $aFONTES, E. P. B.
700 1 $aGROSSI-DE-SA, M. F.
773 $tFrontiers in Plant Science$gv. 11, article 1228, 2020.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Biblioteca |
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| Registros recuperados : 34 | |
| 1. |  | ANDRADE, E. C. de; ALVES JÚNIOR, M.; MANHANI, G. G.; FONTES, E. P. B.; ZERBINI, M. Identificação de determinantes genéticos envolvidos na indução de sintomas por vírus. Fitopatologia Brasileira, Brasília, DF, v. 32, p. 93-94, ago. 2007. Disponível para consulta.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 3. | | VALENTE, M. A. S.; FARIA, J. A. Q. A.; ARAGÃO, F.; FIETTO, L. G.; FONTES, E. P. B. Binding protein (Bip)-mediated increases in water deficit tolerance: the soybean case. In: ANNUAL MEETING OF THE SBBq, 36.; IUBMB CONFERENCE, 10., 2007, Salvador, BA. Infectious diseases: biochemistry of parasites, vectors and hosts: program and abstracts. São Paulo, SP: Brazilian Society for Biochemistry and Molecular Biology, 2007.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 4. | | BASSO, M. F.; SILVA, J. C. F.; FAJARDO, T. V. M.; FONTES, E. P. B.; ZERBINI, F. M. A novel, highly divergent ssDNA virus identified in Brazil infecting apple, pear and grapevine. Virus Research, v. 14, n. 210, p. 27-33, July 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Uva e Vinho. |
|  |
| 5. | | VALENTE, M. A. S.; FARIA, J. A. A. F.; FIETTO, L. G.; ARAGÃO, F. J. L.; FONTES, E. P. B. Ectopic expression of Bip in soybean enhances tolerance to drought. In: ANNUAL MEETING OF SBBQ, 37.; CONGRESS OF THE PABMB, 11., 2008, Águas de Lindóia, SP. Abstracts... Águas de Lindóia: SBBq, 2008. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 6. | | FERNANDES, J. J.; BRAZ, A. S. K.; KRAUSE, R.; FONTES, E. P. B.; AGASIE, I. C. B.; ZERBINI, F. M. Partial host range of TGV-Ub: a new tomato-infecting geminivirus from Uberlandia-MG. Fitopatologia Brasileira, Brasilia, DF, v. 22, p. 334, ago. 1997. Suplemento. Resumo 597. Congresso Brasileiro de Fitopatologia, 30., 1997.| Biblioteca(s): Embrapa Hortaliças. |
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| 7. | | FONTES, E. P. B.; SILVA, C. J.; CAROLINO, S. M. B.; FIGUEREDO, J. D. F.; BATISTA, D. P. O. A soybean binding protein (BiP) homolog is temporally regulated in soybean seeds and associates detectably with normal storage proteins in vitro. Brazilian Journal of Genetics, Ribeirão Preto, v. 19, n. 2, p. 305-312, 1996.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 8. | | COCO, D.; CALIL, I. P.; BRUSTOLINI, O. J. B.; SANTOS, A. A.; INOUE-NAGATA, A. K.; FONTES, E. P. B. Soybean chloritic spot virus, a novel begomovirus infeeting soybean in Brazil. Archives of Virology, New York, v. 158, n. 2, p. 457-462 Feb. 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Hortaliças. |
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| 9. | | COCO, D.; CALIL, I. P.; BRUSTOLINI, O. J. B.; SANTOS, A. A.; NAGATA, A. K. I.; FONTES, E. P. B. Soybean chlorotic spot virus, a novel begomovírus infecting soybean in Brazil. Archives of Virology, v. 158, p. 457-462, Feb. 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Hortaliças. |
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| 11. | | MORAIS, A. T.; MENEZES, C. C.; VIANNA, G. R.; FONTES, E. P. B.; VAEZ, J. R.; ARAGÃO, F. J. L. Transformação genética de soja [Glycine max L. (MERRIL)] visando tolerância à seca. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 9., 2004, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2004. p. 78.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 12. | | VALENTE, M. A. S.; FARIA, J. A. Q. A.; CAROLINO, S. M. B.; ARAGÃO, F. J. L.; OLIVEIRA, M. L.; FONTES, E. P. B. Binding protein (Bip) ectopic expression in soybean: correlation with storage protein syntheis and water stress tolerance. In: REUNIÃO ANUAL: SOCIEDADE BRASILEIRA DE BIOQUÍMICA E BIOLOGIA MOLECULAR, 34., 2005, Águas de Lindóia. Programas e resumos... São Paulo: Sociedade Brasileira de Bioquímica e Biologia Molecular, 2005.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 13. | | MENDES, G. C.; REIS, P. A. B.; CALIL, I. P.; CARVALHO, H. H.; ARAGAO, F. J. L.; FONTES, E. P. B. GmNAC30 and GmNAC81 integrate the endoplasmic reticulum stress- and osmotic stress-induced cell death responses through a vacuolar processing enzyme. PNAS, v. 110, n. 48, p. 19627-19632, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 14. | | MELLO, R. N.; COTRIM, M. A. A.; LOPES, E. F.; MOREIRA, A. G.; CONTIN, F. S.; FONTES, E. P. B.; ALMEIDA, A. M. R.; ZERBINI, F. M. Survey of begomoviruses associated with soybean and identification of Sida mottle virus (SiMov) infecting this crop in Brazil. Virus Reviews & Research, São Paulo, v. 7, n. 1, p. 157, 2002.| Biblioteca(s): Embrapa Soja. |
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| 15. | | KRAUSE, R.; FERNANDES, J. J.; AVILA, A. C.; BEZERRA, I. C.; RIBEIRO, S. G.; RESENDE, R. O.; LIMA, M. F.; FONTES, E. P. B.; ZERBINI, F. M. Widespread occurrence of tomato geminiviruses in Brazil, associated with new biotype of the whitefly vector. In: INTERNATIONAL WORKSHOP ON BEMISIA AND GEMINIVIRAL DISEASES, 2., 1998, San Juan, Puerto Rico. Program & abstracts... San Juan, 1998. p.P-46.| Biblioteca(s): Embrapa Hortaliças. |
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| 16. | | RODRIGUES, J. M.; COUTINHO, F. S.; SANTOS, D. S. dos; VITAL, C. E.; RAMOS, J. R. L. S.; REIS, P. B.; OLIVEIRA, M. G. A.; REIS, A. M. dos; FONTES, E. P. B.; RAMOS, H. J. O. BiP-overexpressing soybean plants display accelerated hypersensitivity response (HR) affecting the SA-dependent sphingolipid and flavonoid pathways. Phytochemistry (Oxford), v. 185, article 112704, 2021. Na publicação: Angela Mehta.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 17. | | MELO, B. P.; LOURENCO, I. T.; FRAGA, O. T.; PINHEIRO, L. B.; LINS, C. B. de J.; MORGANTE, C. V.; ENGLER, J. A.; REIS, P. A. B.; SA, M. F. G. de; FONTES, E. P. B. Contrasting roles of GmNAC065 and GmNAC085 in natural senescence, plant development, multiple stresses and cell death responses. Scientific Reports, v. 11, 11178, 2021. Na publicação: Isabela Tristan Lourenço-Tessutti; Maria Fátima Grossi-de-Sá.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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| 18. | | LIMA, L. L.; BALBI, B. P.; MESQUITA, R. O.; SILVA, J. C. F. da; COUTINHO, F. S.; CARMO, F. M. S.; VITAL, C. E.; MEHTA, A.; LOUREIRO, M. E.; FONTES, E. P. B.; BARROS, E. G.; RAMOS, H. J. O. Proteomic and metabolomic analysis of a drought tolerant soybean cultivar from Brazilian savanna. Crop Breeding, Genetics and Genomics, v. 1, article e190022, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: C - 0 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 19. | | ZERBINI, F. M.; FONTES, E. P. B.; AVILA, A. C. de; BEZERRA I. C.; RIBEIRO, S. G.; RESENDE, R. O.; LIMA, M. F.; FARIA, J. C.; FERNANDES, J. J.; GOULART FILHO, L. R. The emergence of tomato geminiviruses in Brazil: the need for a collaborative research effort. IN: INTERNATIONAL WORKSHOP ON BEMISIA AND GEMINIVIRAL DISEASES, 2., 1998, San Juan, Puerto Rico. Program & abstracts... San Juan, 1998. p.L-106.| Biblioteca(s): Embrapa Hortaliças. |
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| 20. | | ZERBINI, F. M.; FONTES, E. P. B.; AVILA, A. C. de; BEZERRA, I. C.; RIBEIRO, S. G.; RESENDE, R. O.; LIMA, M. F.; FARIA, J. C.; FERNANDES, J. J.; GOULART FILHO, L. R. The emergence of tomato geminiviruses in Brazil: the need for a collaborative research effort. IN: INTERNATIONAL WORKSHOP ON BEMISIA AND GEMINIVIRAL DISEASES, 2., 1998, San Juan, Puerto Rico. Program & abstracts... San Juan, 1998. p. L-106.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| Registros recuperados : 34 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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