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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia; Embrapa Meio-Norte. |
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Data corrente: |
01/11/2022 |
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Data da última atualização: |
01/11/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FERREIRA, T. M. M.; FERREIRA FILHO, J. A.; LEAO, A. P.; SOUSA, C. A. F. de; SOUZA JUNIOR, M. T. |
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Afiliação: |
THALITA MASSARO MALHEIROS FERREIRA; JAIRE ALVES FERREIRA FILHO; ANDRE PEREIRA LEAO, CNPAE; CARLOS ANTONIO FERREIRA DE SOUSA, CPAMN; MANOEL TEIXEIRA SOUZA JUNIOR, CNPAE. |
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Título: |
Structural and functional analysis of stressinducible genes and their promoters selected from young oil palm (Elaeis guineensis) under salt stress. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
BMC Genomics, v. 23, n. 735, p. 1-13, 2022. |
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DOI: |
https://doi.org/10.1186/s12864-022-08926-6 |
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Idioma: |
Inglês |
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Conteúdo: |
Background Soil salinity is a problem in more than 100 countries across all continents. It is one of the abiotic stress that threatens agriculture the most, negatively affecting crops and reducing productivity. Transcriptomics is a technology applied to characterize the transcriptome in a cell, tissue, or organism at a given time via RNA-Seq, also known as full-transcriptome shotgun sequencing. This technology allows the identification of most genes expressed at a particular stage, and different isoforms are separated and transcript expression levels measured. Once determined by this technology, the expression profile of a gene must undergo validation by another, such as quantitative realtime PCR (qRT-PCR). This study aimed to select, annotate, and validate stress-inducible genes?and their promoters?differentially expressed in the leaves of oil palm (Elaeis guineensis) plants under saline stress. Results The transcriptome analysis led to the selection of 14 genes that underwent structural and functional annotation, besides having their expression validated using the qRT-PCR technique. When compared, the RNA-Seq and qRT-PCR profiles of those genes resulted in some inconsistencies. The structural and functional annotation analysis of proteins coded by the selected genes showed that some of them are orthologs of genes reported as conferring resistance to salinity in other species. There were those coding for proteins related to the transport of salt into and out of cells, transcriptional regulatory activity, and opening and closing of stomata. The annotation analysis performed on the promoter sequence revealed 22 distinct types of cis-acting elements, and 14 of them are known to be involved in abiotic stress. Conclusion This study has helped validate the process of an accurate selection of genes responsive to salt stress with a specific and predefined expression profile and their promoter sequence. Its results also can be used in moleculargenetics-assisted breeding programs. In addition, using the identified genes is a window of opportunity for strategies trying to relieve the damages arising from the salt stress in many glycophyte crops with economic importance. MenosBackground Soil salinity is a problem in more than 100 countries across all continents. It is one of the abiotic stress that threatens agriculture the most, negatively affecting crops and reducing productivity. Transcriptomics is a technology applied to characterize the transcriptome in a cell, tissue, or organism at a given time via RNA-Seq, also known as full-transcriptome shotgun sequencing. This technology allows the identification of most genes expressed at a particular stage, and different isoforms are separated and transcript expression levels measured. Once determined by this technology, the expression profile of a gene must undergo validation by another, such as quantitative realtime PCR (qRT-PCR). This study aimed to select, annotate, and validate stress-inducible genes?and their promoters?differentially expressed in the leaves of oil palm (Elaeis guineensis) plants under saline stress. Results The transcriptome analysis led to the selection of 14 genes that underwent structural and functional annotation, besides having their expression validated using the qRT-PCR technique. When compared, the RNA-Seq and qRT-PCR profiles of those genes resulted in some inconsistencies. The structural and functional annotation analysis of proteins coded by the selected genes showed that some of them are orthologs of genes reported as conferring resistance to salinity in other species. There were those coding for proteins related to the transport of salt into and out of cells, trans... Mostrar Tudo |
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Palavras-Chave: |
QRT-PCR; RNA-Seq. |
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Thesaurus Nal: |
Abiotic stress; Salinity; Transcriptomics. |
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Categoria do assunto: |
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X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02977naa a2200241 a 4500
001 2147944
005 2022-11-01
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1186/s12864-022-08926-6$2DOI
100 1 $aFERREIRA, T. M. M.
245 $aStructural and functional analysis of stressinducible genes and their promoters selected from young oil palm (Elaeis guineensis) under salt stress.$h[electronic resource]
260 $c2022
520 $aBackground Soil salinity is a problem in more than 100 countries across all continents. It is one of the abiotic stress that threatens agriculture the most, negatively affecting crops and reducing productivity. Transcriptomics is a technology applied to characterize the transcriptome in a cell, tissue, or organism at a given time via RNA-Seq, also known as full-transcriptome shotgun sequencing. This technology allows the identification of most genes expressed at a particular stage, and different isoforms are separated and transcript expression levels measured. Once determined by this technology, the expression profile of a gene must undergo validation by another, such as quantitative realtime PCR (qRT-PCR). This study aimed to select, annotate, and validate stress-inducible genes?and their promoters?differentially expressed in the leaves of oil palm (Elaeis guineensis) plants under saline stress. Results The transcriptome analysis led to the selection of 14 genes that underwent structural and functional annotation, besides having their expression validated using the qRT-PCR technique. When compared, the RNA-Seq and qRT-PCR profiles of those genes resulted in some inconsistencies. The structural and functional annotation analysis of proteins coded by the selected genes showed that some of them are orthologs of genes reported as conferring resistance to salinity in other species. There were those coding for proteins related to the transport of salt into and out of cells, transcriptional regulatory activity, and opening and closing of stomata. The annotation analysis performed on the promoter sequence revealed 22 distinct types of cis-acting elements, and 14 of them are known to be involved in abiotic stress. Conclusion This study has helped validate the process of an accurate selection of genes responsive to salt stress with a specific and predefined expression profile and their promoter sequence. Its results also can be used in moleculargenetics-assisted breeding programs. In addition, using the identified genes is a window of opportunity for strategies trying to relieve the damages arising from the salt stress in many glycophyte crops with economic importance.
650 $aAbiotic stress
650 $aSalinity
650 $aTranscriptomics
653 $aQRT-PCR
653 $aRNA-Seq
700 1 $aFERREIRA FILHO, J. A.
700 1 $aLEAO, A. P.
700 1 $aSOUSA, C. A. F. de
700 1 $aSOUZA JUNIOR, M. T.
773 $tBMC Genomics$gv. 23, n. 735, p. 1-13, 2022.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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Biblioteca |
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Origem |
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Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
29/02/2024 |
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Data da última atualização: |
04/03/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
CHIARAVALLOTI, R. M.; TOMAS, W. M.; AKRE, T.; MORATO, R. G.; CAMILO, A. R.; GIORDANO, A. J.; LEIMGRUBER, P. |
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Afiliação: |
RAFAEL MORAIS CHIARAVALLOTI, UNIVERSITY COLLEGE LONDON; WALFRIDO MORAES TOMAS, CPAP; TOM AKRE, Conservation Biology Institute; RONALDO GONÇALVES MORATO, INSTITUTO CHICO MENDES DE CONSERVAÇAO DA BIODIVERSIDADE; ANDRE RESTEL CAMILO, INSTITUTO DE PESQUISAS ECOLOGICAS; ANTHONY J. GIORDANO, S.P.E.C.I.E.S; PETER LEIMGRUBER, CONSERVATION ECOLOGY CENTER. |
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Título: |
Achieving conservation through cattle ranching: the case of the Brazilian Pantanal. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Conservation Science and Practice, p. 1–11, 2023. |
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DOI: |
https://doi.org/10.1111/csp2.13006 |
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Idioma: |
Inglês |
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Notas: |
Online First. |
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Conteúdo: |
Cattle ranching in the 140,000 km 2 Brazilian Pantanal is considered one of the most important cases of sustainable use of natural resources in the global south. The region has had a successful history of balancing environmental protection with the production of >3.8 million cattle. However, global change, infrastructure projects, and deforestation, threaten the sustainable use of the Pantanal. Here, using Ostrom's design principles as a framework, we interviewed 49 local stakeholders and conducted a review of secondary information aiming to evaluate the sustainability of cattle ranching practices across the region and the threats to it. We show that well-defined property boundaries, congruence between appropriation and provision rules through low-intensity cattle ranching, and co-management of resources, are all key components for achieving sustainability in the Pantanal. However, we documented shortcomings in satisfying critical aspects of Orstrom's design principles. Specifically, we argue that the Pantanal needs better biodiversity and behavior monitoring, the creation of platforms or mechanisms to solve local conflicts around resource access and use, recognition by governments and international bodies of the local efforts to promote local sustainability, and the creation of networks effectively connecting existing sustainability initiatives. |
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Thesagro: |
Desenvolvimento Sustentável; Pecuária. |
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Thesaurus NAL: |
Cattle production; Sustainable development; Sustainable land management; Wetlands. |
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Categoria do assunto: |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1162390/1/Conservat-Sci-and-Prac-2023-Chiaravalloti-1.pdf
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Marc: |
LEADER 02235naa a2200289 a 4500
001 2162390
005 2024-03-04
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1111/csp2.13006$2DOI
100 1 $aCHIARAVALLOTI, R. M.
245 $aAchieving conservation through cattle ranching$bthe case of the Brazilian Pantanal.$h[electronic resource]
260 $c2023
500 $aOnline First.
520 $aCattle ranching in the 140,000 km 2 Brazilian Pantanal is considered one of the most important cases of sustainable use of natural resources in the global south. The region has had a successful history of balancing environmental protection with the production of >3.8 million cattle. However, global change, infrastructure projects, and deforestation, threaten the sustainable use of the Pantanal. Here, using Ostrom's design principles as a framework, we interviewed 49 local stakeholders and conducted a review of secondary information aiming to evaluate the sustainability of cattle ranching practices across the region and the threats to it. We show that well-defined property boundaries, congruence between appropriation and provision rules through low-intensity cattle ranching, and co-management of resources, are all key components for achieving sustainability in the Pantanal. However, we documented shortcomings in satisfying critical aspects of Orstrom's design principles. Specifically, we argue that the Pantanal needs better biodiversity and behavior monitoring, the creation of platforms or mechanisms to solve local conflicts around resource access and use, recognition by governments and international bodies of the local efforts to promote local sustainability, and the creation of networks effectively connecting existing sustainability initiatives.
650 $aCattle production
650 $aSustainable development
650 $aSustainable land management
650 $aWetlands
650 $aDesenvolvimento Sustentável
650 $aPecuária
700 1 $aTOMAS, W. M.
700 1 $aAKRE, T.
700 1 $aMORATO, R. G.
700 1 $aCAMILO, A. R.
700 1 $aGIORDANO, A. J.
700 1 $aLEIMGRUBER, P.
773 $tConservation Science and Practice, p. 1–11, 2023.
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Embrapa Pantanal (CPAP) |
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