| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Arroz e Feijão. |
|
Data corrente: |
20/11/2019 |
|
Data da última atualização: |
24/04/2020 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
PAIVA, B. A. R. de; WENDLAND, A.; TEIXEIRA, N. C.; FERREIRA, M. A. S. V. |
|
Afiliação: |
BRUNA ALICIA RAFAEL DE PAIVA, UNB; ADRIANE WENDLAND FERREIRA, CNPAF; NARA CRISTINA TEIXEIRA, bolsista CNPAF; MARISA A. S. V. FERREIRA, UNB. |
|
Título: |
Rapid detection of Xanthomonas citri pv. fuscans and Xanthomonas phaseoli pv. phaseoli in common bean by loop-mediated isothermal amplification. |
|
Ano de publicação: |
2020 |
|
Fonte/Imprenta: |
Plant Disease, v. 104, n. 1, p. 198-203, Jan. 2020. |
|
ISSN: |
0191-2917 |
|
DOI: |
10.1094/PDIS-02-19-0325-RE |
|
Idioma: |
Inglês |
|
Conteúdo: |
A single loop-mediated isothermal amplification (LAMP) assay was developed for specific detection of both pathogens that cause bacterial blight in common bean, Xanthomonas phaseoli pv. phaseoli (Xpp) and Xanthomonas citri pv. fuscans (Xcf). The objective was to provide a simple, easy-to-use, specific, and sensitive method to investigate the presence of one or both pathogens in plant material and seeds for routine diagnosis. The detection limits for both pathogens were 10 CFU/ml for cell suspensions and 1 fg of DNA, whereas in conventional PCR, the primers detected up to 105 CFU/ml and 1 ng of DNA. Specificity was confirmed by testing DNA from bean leaves, other Xanthomonas species, common fungal and bacterial bean pathogens, and bacteria from the leaf microbiota. The method was tested with bean leaves inoculated with Xpp, and the pathogen could be detected from 4 h up to 15 days postinoculation, even before disease symptoms were visible. When the method was applied to bacterium detection (Xpp or Xcf) in seed lots from infected plants, the bacterium detection rate was 100% (24 of 24). The pathogens were detected in seeds incubated for just 1 h in saline solution (0.85%), reducing the time needed for bacterium detection. The LAMP assay could be useful as a tool in bean bacterial blight management. Rapid and sensitive detection of bacteria in bean seed lots would reduce the risks of planting highly contaminated seeds in environments favorable to blight multiplication. |
|
Thesagro: |
Bactéria; Doença de Planta; Feijão; Phaseolus Vulgaris; Xanthomonas Phaseoli. |
|
Thesaurus Nal: |
Bacterial diseases of plants; Beans; Fields; Loop-mediated isothermal amplification; Oilseeds; Pathogen identification; Plant diseases and disorders. |
|
Categoria do assunto: |
H Saúde e Patologia |
|
Marc: |
LEADER 02520naa a2200325 a 4500
001 2114711
005 2020-04-24
008 2020 bl uuuu u00u1 u #d
022 $a0191-2917
024 7 $a10.1094/PDIS-02-19-0325-RE$2DOI
100 1 $aPAIVA, B. A. R. de
245 $aRapid detection of Xanthomonas citri pv. fuscans and Xanthomonas phaseoli pv. phaseoli in common bean by loop-mediated isothermal amplification.$h[electronic resource]
260 $c2020
520 $aA single loop-mediated isothermal amplification (LAMP) assay was developed for specific detection of both pathogens that cause bacterial blight in common bean, Xanthomonas phaseoli pv. phaseoli (Xpp) and Xanthomonas citri pv. fuscans (Xcf). The objective was to provide a simple, easy-to-use, specific, and sensitive method to investigate the presence of one or both pathogens in plant material and seeds for routine diagnosis. The detection limits for both pathogens were 10 CFU/ml for cell suspensions and 1 fg of DNA, whereas in conventional PCR, the primers detected up to 105 CFU/ml and 1 ng of DNA. Specificity was confirmed by testing DNA from bean leaves, other Xanthomonas species, common fungal and bacterial bean pathogens, and bacteria from the leaf microbiota. The method was tested with bean leaves inoculated with Xpp, and the pathogen could be detected from 4 h up to 15 days postinoculation, even before disease symptoms were visible. When the method was applied to bacterium detection (Xpp or Xcf) in seed lots from infected plants, the bacterium detection rate was 100% (24 of 24). The pathogens were detected in seeds incubated for just 1 h in saline solution (0.85%), reducing the time needed for bacterium detection. The LAMP assay could be useful as a tool in bean bacterial blight management. Rapid and sensitive detection of bacteria in bean seed lots would reduce the risks of planting highly contaminated seeds in environments favorable to blight multiplication.
650 $aBacterial diseases of plants
650 $aBeans
650 $aFields
650 $aLoop-mediated isothermal amplification
650 $aOilseeds
650 $aPathogen identification
650 $aPlant diseases and disorders
650 $aBactéria
650 $aDoença de Planta
650 $aFeijão
650 $aPhaseolus Vulgaris
650 $aXanthomonas Phaseoli
700 1 $aWENDLAND, A.
700 1 $aTEIXEIRA, N. C.
700 1 $aFERREIRA, M. A. S. V.
773 $tPlant Disease$gv. 104, n. 1, p. 198-203, Jan. 2020.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 55 | |
| 3. |  | LIMA, M. F.; FERREIRA, M. A. S. V.; MOREIRA, W. A.; DIANESE, J. C. Bacterial canker of grapevine in Brazil. Fitopatologia Brasileira, Brasília, DF, v. 24, n. 3, p. 440-443, set. 1999.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - B |
| Biblioteca(s): Embrapa Semiárido. |
|    |
| 4. | | FERREIRA, M. A. S. V.; TOOLEY, P. W.; HATZILOUKAS, E.; CASTRO, C.; SHAAD, N. W. Isolation of a species-specific mitochondrial DNA sequence for identification of Tilletia indica, the Karnal bunt of wheat fungus. Applied and Environmental Microbiology, Washington, v.62, n.1, p.87-93, 1996.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 5. | | FREITAS, A. C.; MIRANDA, T. D.; BARBOSA, M. A. G.; FERREIRA, M. A. S. V. Limite de detecção de Xanthomonas campestris pv. viticola por nested-PCR em frutos assintomáticos de videiras. Tropical Plant Pathology, Brasília, DF, v. 36, 2011. p. 1039. 1 CD-ROM. Suplemento. Edição dos Resumos do 44 Congresso Brasileiro de Fitopatologia, Bento Gonçalves, ago. 2011.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
| |
| 6. | | COSTA, S. B.; FERREIRA, M. A. S. V.; BOITEUX, L. S.; LOPES, C. A. Caracterização molecular de isolados de Ralstonia solanacearum da região Amazônica através de BOX-PCR. Fitopatologia Brasileira, Brasília, v. 28, p. 237, ago. 2003. Suplemento. Trabalho apresentado no 36º Congresso Brasileiro de Fitopatologia, 2003, Brasília. Resumo.| Biblioteca(s): Embrapa Hortaliças. |
| |
| 7. | | ANDRADE, E. M.; UESUGI, C. H.; UENO, B.; FERREIRA, M. A. S. V. Caracterização morfocultural e molecular de isolados de Colletotrichum gloeosporioides patogênicos ao mamoeiro. Fitopatologia Brasileira, Brasília, DF, v. 32, n.1, p. 21-31, jan./fev, 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - A |
| Biblioteca(s): Embrapa Clima Temperado. |
| |
| 8. | | TEIXEIRA, A. C. O.; MARQUES, A. S. A.; FERREIRA, M. A. S. V. Caracterização de isolados de Erwinia psidii do Distrito Federal. Fitopatologia Brasileira, v. 31, suppl., ago. 2006. Anais do: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 39.; ANNUAL MEETING OF THE BRAZILIAN PHYTOPATHOLOGICAL SOCIETY, 39., 2006, Salvador, BA. p. S185.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
|  |
| 10. | | PEREIRA, R. C.; ARÚJO, E. R.; QUEZADO-DUVAL, A. M.; FERREIRA, M. A. S. V. Caracterização de isolados de Xanthomonas spp. oriundos de lavouras de tomate para consumo in natura quanto aos perfis de BOX-PCR. Tropical Plant Pathology, Brasília, DF, v. 34, p. S9, ago. 2009. Suplemento. Resumo nº. 30. Trabalho apresentado no 42. Congresso Brasileiro de Fitopatologia, Rio de Janeiro.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Hortaliças. |
| |
| 12. | | MENDES, M. A. S.; FERREIRA, M. A. S. V.; ALMEIDA, M. F. Podridão de frutos de romã (Punica Gramatum) associado a Coniella gramati. Fitopatologia Brasileira, Brasília, DF, v. 18, p. 332, ago. 1993. Edição dos Resumos do XXVI Congresso Brasileiro de Fitopatologia, Aracajú, ago. 1993.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 15. | | ARAÚJO, E. R.; PEREIRA, R. C.; QUEZADO-DUVAL, A. M.; FERREIRA, M. A. S. V. Sensibilidade in vitro de isolados de Xanthomonas perforans a cobre e estreptomicina. Tropical plant Pathology, Brasília, DF, v. 34, p. S94, ago. 2009. Suplemento. Resumo 342. Trabalho apresentado no 42. Congresso Brasileiro de Fitopatologia, Rio de Janeiro.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Hortaliças. |
| |
| Registros recuperados : 55 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
