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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
02/10/2012 |
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Data da última atualização: |
25/02/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DOMINGUES, L. N.; BRASIL, B. dos S. A. F.; BELLO, A. C. P. de P.; CUNHA, A. P. da; BARROS, A. T. M. de; LEITE, R. C.; SILAGHI, C.; PFISTER, K.; PASSOS, L. M. F. |
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Afiliação: |
LUÍSA NOGUEIRA DOMINGUES, UFMG; BRUNO DOS SANTOS ALVES FIGUEIREDO BRASIL, UFMG; ANA CRISTINA PASSOS DE PAIVA BELLO, UFMG; ARILDO PINTO DA CUNHA, UFMG; ANTONIO THADEU MEDEIROS DE BARROS, CPAP; ROMÁRIO CERQUEIRA LEITE, UFMG; CORNELIA SILAGHI, UNIVERSITAT MUNCHEN; KURT PFISTER, UNIVERSITAT MUNCHEN; LYGIA MARIA FRICHE PASSOS, UFMG - UNIVERSITAT MUNCHEN. |
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Título: |
Survey of pyrethroid and organophosphate resistance in Brazilian field populations of Rhipicephalus (Boophilus) microplus: Detection of C190A mutation in domain II of the para-type sodium channel gene. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Veterinary Parasitology, v. 189, n.2-4, p. 327-332, 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
The cattle tick Rhipicephalus (Boophilus) microplus causes expressive damage to livestock in Brazil and other countries. Its control is becoming more difficult due to the development of resistance in populations. Early detection of resistance can help in developing effective control strategies. This study evaluated the susceptibility of R. microplus to cypermethrin and chlorpyriphos and was the first attempt to identify the mechanism of resistance (target site insensitivity) in cattle tick populations from Minas Gerais state (Southeastern Brazil). Engorged female ticks were collected from 10 ranches within the state of Minas Gerais, and susceptibility was evaluated with the larval packet test (LPT) using technical grade cypermethrin and chlorpyriphos. It was possible to analyze LPT results of seven populations. Target site insensitivity was investigated in all 10 isolates by using molecular approaches for detection of the T2134A substitution within the domain III S6 segment and the C190A in the domain II S4-5 linker from the para-type sodium channel gene. LPT showed that all seven populations were resistant to cypermethrin with resistance ratio (RR) ranging from 16.0 to 25.0 and 85.7% were resistant to chlorpyriphos (RR = 2.2?15.6). Although the T2134A mutation was not detected, the C190A mutation was highly prevalent, being present in 82?100% of the alleles sampled in field populations. A significant correlation was found between the LC50 values for cypermethrin and the frequency of the C190A mutation suggesting that it might be responsible for the phenotypic resistance detected. MenosThe cattle tick Rhipicephalus (Boophilus) microplus causes expressive damage to livestock in Brazil and other countries. Its control is becoming more difficult due to the development of resistance in populations. Early detection of resistance can help in developing effective control strategies. This study evaluated the susceptibility of R. microplus to cypermethrin and chlorpyriphos and was the first attempt to identify the mechanism of resistance (target site insensitivity) in cattle tick populations from Minas Gerais state (Southeastern Brazil). Engorged female ticks were collected from 10 ranches within the state of Minas Gerais, and susceptibility was evaluated with the larval packet test (LPT) using technical grade cypermethrin and chlorpyriphos. It was possible to analyze LPT results of seven populations. Target site insensitivity was investigated in all 10 isolates by using molecular approaches for detection of the T2134A substitution within the domain III S6 segment and the C190A in the domain II S4-5 linker from the para-type sodium channel gene. LPT showed that all seven populations were resistant to cypermethrin with resistance ratio (RR) ranging from 16.0 to 25.0 and 85.7% were resistant to chlorpyriphos (RR = 2.2?15.6). Although the T2134A mutation was not detected, the C190A mutation was highly prevalent, being present in 82?100% of the alleles sampled in field populations. A significant correlation was found between the LC50 values for cypermethrin and the fre... Mostrar Tudo |
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Palavras-Chave: |
Allele-specific PCR; Cattle tick; Chlorpyriphos; Larval packet test; Target site insensitivity. |
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Thesaurus Nal: |
cypermethrin. |
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Categoria do assunto: |
O Insetos e Entomologia |
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Marc: |
LEADER 02556naa a2200289 a 4500
001 1935239
005 2013-02-25
008 2012 bl --- 0-- u #d
100 1 $aDOMINGUES, L. N.
245 $aSurvey of pyrethroid and organophosphate resistance in Brazilian field populations of Rhipicephalus (Boophilus) microplus$bDetection of C190A mutation in domain II of the para-type sodium channel gene.
260 $c2012
520 $aThe cattle tick Rhipicephalus (Boophilus) microplus causes expressive damage to livestock in Brazil and other countries. Its control is becoming more difficult due to the development of resistance in populations. Early detection of resistance can help in developing effective control strategies. This study evaluated the susceptibility of R. microplus to cypermethrin and chlorpyriphos and was the first attempt to identify the mechanism of resistance (target site insensitivity) in cattle tick populations from Minas Gerais state (Southeastern Brazil). Engorged female ticks were collected from 10 ranches within the state of Minas Gerais, and susceptibility was evaluated with the larval packet test (LPT) using technical grade cypermethrin and chlorpyriphos. It was possible to analyze LPT results of seven populations. Target site insensitivity was investigated in all 10 isolates by using molecular approaches for detection of the T2134A substitution within the domain III S6 segment and the C190A in the domain II S4-5 linker from the para-type sodium channel gene. LPT showed that all seven populations were resistant to cypermethrin with resistance ratio (RR) ranging from 16.0 to 25.0 and 85.7% were resistant to chlorpyriphos (RR = 2.2?15.6). Although the T2134A mutation was not detected, the C190A mutation was highly prevalent, being present in 82?100% of the alleles sampled in field populations. A significant correlation was found between the LC50 values for cypermethrin and the frequency of the C190A mutation suggesting that it might be responsible for the phenotypic resistance detected.
650 $acypermethrin
653 $aAllele-specific PCR
653 $aCattle tick
653 $aChlorpyriphos
653 $aLarval packet test
653 $aTarget site insensitivity
700 1 $aBRASIL, B. dos S. A. F.
700 1 $aBELLO, A. C. P. de P.
700 1 $aCUNHA, A. P. da
700 1 $aBARROS, A. T. M. de
700 1 $aLEITE, R. C.
700 1 $aSILAGHI, C.
700 1 $aPFISTER, K.
700 1 $aPASSOS, L. M. F.
773 $tVeterinary Parasitology$gv. 189, n.2-4, p. 327-332, 2012.
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Registro original: |
Embrapa Pantanal (CPAP) |
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| Registros recuperados : 9 | |
| 2. |  | MENDES, M. C.; CALVO, F. D.; MARTINS, J, R.; DOMINGUES, L. N.; KLAFKE, G. M.; BARROS, A. T. M. de. Caracterización poblacional de la resistencia de la garrapata Rhipicephalus (Boophilus) microplus a la cipermetrina, deltametrina y flumetrina en los estados de Rio Grande do Sul, Minas Gerais y Mato Grosso do Sul, Brasil. In: CONGRESO LATINOAMERICANO DE ACAROLOGÍA, 1, 2012, Puebla. Anais... Puebla: Sociedad Mexicana de Entomologia, 2012. p.264-273.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Pantanal. |
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| 4. | | DOMINGUES, L. N.; BELLO, A. C. P. P.; CUNHA, A. P.; LEITE, P. V. B.; BARROS, A. T. M. de; LEITE, R. C. Caracterização do controle de Haematobia irritans e Rhipicephalus (Boophilus) microplus no Triângulo Mineiro e Alto Paranaíba, Minas Gerais. Pesquisa Veterinária Brasileira, v. 32, n. 12, p. 1246-1252, dezembro, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Pantanal. |
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| 5. | | WANDERLEY, R. P. B.; RIBEIRO, A. C. de C. L.; RODRIGUES, D. S.; BARROS, A. B.; LEITE, P. V. B.; DOMINGUES, L. N.; CUNHA, A. P.; BELLO, A. C. P. P.; LEITE, R. C. Adubação com ureia em pastejo rotacionado e seu efeito no controle do Rhipicephalus (Boophilus) microplus. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 17., 2012, São Luis. Parasitologia veterinária, bem estar e produção animal: anais. São Luis: Colégio Brasileiro de Parasitologia Veterinária, 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 6. | | DOMINGUES, L. N.; CUNHA, A. C. P.; BELLO, A. C. P. P.; DALLA ROSA, R. C.; LEITE, P. V. B.; LEITE, L. B.; RESENDE, T. P.; RODRIGUES, D. S.; BARROS, A. T. M. de; LEITE, R. C. Insecticide susceptibility of Haematobia irritans (Diptera: muscidae) in the western region of Minas Gerais, Brazil. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 16., Campo Grande. Anais... Campo Grande: CBPV, 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pantanal. |
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| 7. | | DOMINGUES, L. N.; BRASIL, B. dos S. A. F.; BELLO, A. C. P. de P.; CUNHA, A. P. da; BARROS, A. T. M. de; LEITE, R. C.; SILAGHI, C.; PFISTER, K.; PASSOS, L. M. F. Survey of pyrethroid and organophosphate resistance in Brazilian field populations of Rhipicephalus (Boophilus) microplus: Detection of C190A mutation in domain II of the para-type sodium channel gene. Veterinary Parasitology, v. 189, n.2-4, p. 327-332, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pantanal. |
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| 8. | | DOMINGUES, L. N.; BELLO, A. C. P. P.; CUNHA, A. C. P.; DALLA ROSA, R. C.; LEITE, P. V. B.; LEITE, L. B.; RESENDE, T. P.; RODRIGUES, D. S.; WANDERLEY, R. P. B.; BARROS, A. B.; BARROS, A. T. M. de; LEITE, R. C. Caracterização do controle de Rhipicephalus (Boophilus) Microplus (Acari: ixodidae) e Haematobia irritans (Diptera: muscidae) na região do Triângulo Mineiro e Alto Paranaíba, Minas Gerais. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 16., Campo Grande. Anais... Campo Grande: CBPV, 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pantanal. |
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| 9. | | RODRIGUES, D. S.; MORAIS, O. R. de; BASTIANETTO, O.; WANDERLEY, R. P. B.; LEITE, P. V. B.; DOMINGUES, L. N.; CUNHA, A. P. da; BELLO, A. C. P. de P.; SILVA, M. X.; SALIBA, E. de O. S.; LEITE, R. C. The use of Stylosanthes guianensis cv. Mineirão and Medicago sativa hays to control gastrintestinal nematodes in feedlot lambs. In: INTERNATIONAL CONFERENCE OF THE WORLD ASSOCIATION FOR THE ADVANCEMENT OF VETERINARY PARASITOLOGY, 23., 2011, Buenos Aires. Proceedings... Buenos Aires: WAAVP, 2011. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| Registros recuperados : 9 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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