02556naa a2200289 a 450000100080000000500110000800800410001910000210006024502060008126000090028752016120029665000170190865300240192565300160194965300180196565300230198365300300200670000280203670000260206470000200209070000240211070000170213470000160215170000160216770000210218377300620220419352392013-02-25       2012    bl ---       0--  u    #d1 aDOMINGUES, L. N.  aSurvey of pyrethroid and organophosphate resistance in Brazilian field populations of Rhipicephalus (Boophilus) microplusbDetection of C190A mutation in domain II of the para-type sodium channel gene.  c2012  aThe cattle tick Rhipicephalus (Boophilus) microplus causes expressive damage to livestock in Brazil and other countries. Its control is becoming more difficult due to the development of resistance in populations. Early detection of resistance can help in developing effective control strategies. This study evaluated the susceptibility of R. microplus to cypermethrin and chlorpyriphos and was the first attempt to identify the mechanism of resistance (target site insensitivity) in cattle tick populations from Minas Gerais state (Southeastern Brazil). Engorged female ticks were collected from 10 ranches within the state of Minas Gerais, and susceptibility was evaluated with the larval packet test (LPT) using technical grade cypermethrin and chlorpyriphos. It was possible to analyze LPT results of seven populations. Target site insensitivity was investigated in all 10 isolates by using molecular approaches for detection of the T2134A substitution within the domain III S6 segment and the C190A in the domain II S4-5 linker from the para-type sodium channel gene. LPT showed that all seven populations were resistant to cypermethrin with resistance ratio (RR) ranging from 16.0 to 25.0 and 85.7% were resistant to chlorpyriphos (RR = 2.2?15.6). Although the T2134A mutation was not detected, the C190A mutation was highly prevalent, being present in 82?100% of the alleles sampled in field populations. A significant correlation was found between the LC50 values for cypermethrin and the frequency of the C190A mutation suggesting that it might be responsible for the phenotypic resistance detected.  acypermethrin  aAllele-specific PCR  aCattle tick  aChlorpyriphos  aLarval packet test  aTarget site insensitivity1 aBRASIL, B. dos S. A. F.1 aBELLO, A. C. P. de P.1 aCUNHA, A. P. da1 aBARROS, A. T. M. de1 aLEITE, R. C.1 aSILAGHI, C.1 aPFISTER, K.1 aPASSOS, L. M. F.  tVeterinary Parasitologygv. 189, n.2-4, p. 327-332, 2012.