| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Semiárido. |
|
Data corrente: |
11/01/2013 |
|
Data da última atualização: |
11/01/2013 |
|
Tipo da produção científica: |
Artigo em Anais de Congresso |
|
Autoria: |
COSTA, D. C. C. da; SANTOS, C. A. F.; SOUZA, S. S. da S.; VITORINO, W. R. da S. |
|
Afiliação: |
DANIELLE CAROLINA CAMPOS DA COSTA; CARLOS ANTONIO FERNANDES SANTOS, CPATSA; SAMILA SAMEA DA SILVA SOUZA; WESLANY ROBERTO DA SILVA VITORINO, CPATSA. |
|
Título: |
Interação genótipo x ambiente para teor de proteína total em linhagens de feijão-caupi |
|
Ano de publicação: |
2012 |
|
Fonte/Imprenta: |
In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMIÁRIDO, 7.; JORNADA DE INICIAÇÃO CIENTÍFICA DA FACEPE/UNIVASF, 1., 2012, Petrolina. Anais... Petrolina: Embrapa Semiárido, 2012. |
|
Páginas: |
p. 123-129. |
|
Descrição Física: |
1 CD-ROM |
|
Série: |
(Embrapa Semiárido. Documentos, 248). |
|
Idioma: |
Português |
|
Conteúdo: |
O objetivo deste trabalho foi avaliar a interação genótipo x ambiente para o teor total de proteínas em linhagens de feijão-caupi, avaliadas em sete ambientes diferentes para possibilitar a seleção, registro e recomendação de novas cultivares para a região do Vale do São Francisco. Foram analisadas amostras de feijão-caupi de experimentos conduzidos em três ambientes irrigados e em quatro ambientes de sequeiro no Nordeste brasileiro. A quantificação de proteínas totais em feijão seco foi realizada pelo método químico de Kjeldhal. Nos dois experimentos foi observada interação significativa genótipo x ambiente (p<0,01) nas linhagens avaliadas. Foram identificadas cinco linhagens com valores médios de proteínas em torno de 30% no grão. Os resultados indicam a real possibilidade da recomendação de cultivares de feijão-caupi com teor de proteína superior a 20% em relação a algumas cultivares comerciais disponíveis para o Vale do São Francisco. |
|
Palavras-Chave: |
Ambiente; Bean; Feijão caupi; Proteínas. |
|
Thesagro: |
Feijão; Genótipo; Vigna Unguiculata. |
|
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/73909/1/carlos-fernandes.pdf
|
|
Marc: |
LEADER 01857nam a2200253 a 4500
001 1944820
005 2013-01-11
008 2012 bl uuuu u00u1 u #d
100 1 $aCOSTA, D. C. C. da
245 $aInteração genótipo x ambiente para teor de proteína total em linhagens de feijão-caupi
260 $aIn: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMIÁRIDO, 7.; JORNADA DE INICIAÇÃO CIENTÍFICA DA FACEPE/UNIVASF, 1., 2012, Petrolina. Anais... Petrolina: Embrapa Semiárido$c2012
300 $ap. 123-129.$c1 CD-ROM
490 $a(Embrapa Semiárido. Documentos, 248).
520 $aO objetivo deste trabalho foi avaliar a interação genótipo x ambiente para o teor total de proteínas em linhagens de feijão-caupi, avaliadas em sete ambientes diferentes para possibilitar a seleção, registro e recomendação de novas cultivares para a região do Vale do São Francisco. Foram analisadas amostras de feijão-caupi de experimentos conduzidos em três ambientes irrigados e em quatro ambientes de sequeiro no Nordeste brasileiro. A quantificação de proteínas totais em feijão seco foi realizada pelo método químico de Kjeldhal. Nos dois experimentos foi observada interação significativa genótipo x ambiente (p<0,01) nas linhagens avaliadas. Foram identificadas cinco linhagens com valores médios de proteínas em torno de 30% no grão. Os resultados indicam a real possibilidade da recomendação de cultivares de feijão-caupi com teor de proteína superior a 20% em relação a algumas cultivares comerciais disponíveis para o Vale do São Francisco.
650 $aFeijão
650 $aGenótipo
650 $aVigna Unguiculata
653 $aAmbiente
653 $aBean
653 $aFeijão caupi
653 $aProteínas
700 1 $aSANTOS, C. A. F.
700 1 $aSOUZA, S. S. da S.
700 1 $aVITORINO, W. R. da S.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Semiárido (CPATSA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Meio Ambiente. |
|
Data corrente: |
04/04/2017 |
|
Data da última atualização: |
29/06/2017 |
|
Tipo da produção científica: |
Resumo em Anais de Congresso |
|
Autoria: |
PETRARCA, M. H.; ROSA, M. A.; QUEIROZ, S. C. do N. de; GODOY, H. T. |
|
Afiliação: |
M. H. PETRARCA, FEA/Unicamp; MARIA APARECIDA ROSA, CNPMA; SONIA CLAUDIA DO N DE QUEIROZ, CNPMA; H. T. GODOY. |
|
Título: |
Development, uncertainty estimation and application of a LC-MS/MS method for simultaneous determination of acrylamide and 4hydroxy2,5-dimethyl-3(2H)-furanone in baby food. |
|
Ano de publicação: |
2016 |
|
Fonte/Imprenta: |
In: IBERO-AMERICAN, 1.; BrMASS CONFERENCE ON MASS SPECTROMETRY, 6., 2016, Rio de Janeiro. Resumos Eletrônicos... São Paulo: Sociedade Brasileira de Espectrometria de Massas, 2016. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Acrylamide, a heatgenerated food contaminant, has been associated with neurotoxic effects in humans and was classified under group 2A as a probable human carcinogen by the International Agency Research on Cancer (IARC) [1]. Additionally, hydroxy2,5dimethyl3(2H)furanone (DMHF), a furanic compound, has demonstrated genotoxic effects in vitro as also in vivo [2]. In this study, a new accurate liquid chromatographytandem mass spectrometry method was developed for simultaneous analysis of acrylamide and DMHF in baby food. To the best of our knowledge, this is the first study on DMHF in baby foods as well as pioneer in acrylamide monitoring in baby foods from Brazil. For this, a sample preparation method was optimized, which involves acetonitrile-based extraction, followed by dispersive solidphase extraction (dSPE) using primary and secondary amine sorbent (PSA) and then, a final cleanup of extract by solid phase extraction (SPE). An ACQUITY ultraperformance liquid chromatography (UPLC) system interfaced to a Quattro Premier XE triple quadrupole mass spectrometer with electrospray ionization (ESI) source (WATERS) was employed for analysis. The chromatographic separation was achieved on a C8 column (150 mm x 2.1 mm i.d., 5?m particle size) at 30ºC with a flow rate of 0.2 ml/min, and the injection volume was 20 ?l. The mobile phases consisted of MilliQ water (A) and acetonitrile (B), both containing 0.01% of formic acid, and the elution gradient employed was as follows: 0 min, 5% B? 5 min, 50% B? 5.1 min, 100% B? 6?10 min 5% B, resulting in a total run time of 10 min. Data acquisition was carried out in selective reaction monitoring (SRM) mode employing ESI in positive mode, whose conditions were: capillary voltage 3 KV? temperatures of source at 120°C and desolvation at 400°C and gas flow 500 L/h. The collision energy and cone voltage were 10 and 25 V. The m/z 55 and 44 (acrylamide) and m/z 101 and 83 (DMHF) were used respectively for quantitative and confirmative purposes. As a great result, the optimized sample preparation method provided sufficient removal of potential analytical interfering commonly present in fruitbased matrices, the amino acid valine, without affecting the sensitivity, trueness and precision of the method. Limits of detection (LOD) and quantification (LOQ) of 10 and 20 ?g/kg were obtained for both analytes. Linearity in solventonly and matrixmatched calibration curves was achieved in the range between 10 and 300 ?g/kg, with determination coefficients higher than 0.99. Matrix effects, calculated from slopes obtained for solvent and matrixmatched calibrations, were observed for acrylamide (?45%) and DMHF(?11%). Recoveries between 75 and 115% were obtained for the two studied compounds from blank baby foods spiked at 20 (LOQ), 100 and 200 ?g/kg. Precision, under repeatability and withinlaboratory reproducibility conditions, was verified with RSD values < 20%. In addition, by using data obtained from inhouse validation, was possible to estimate the expanded measurement uncertainty for the levels of acrylamide and DMHF detected in the samples, whose values were lower than 25%. The applicability of proposed analytical method was evaluated by analyzing 20 baby food samples available in Brazilian markets. MenosAcrylamide, a heatgenerated food contaminant, has been associated with neurotoxic effects in humans and was classified under group 2A as a probable human carcinogen by the International Agency Research on Cancer (IARC) [1]. Additionally, hydroxy2,5dimethyl3(2H)furanone (DMHF), a furanic compound, has demonstrated genotoxic effects in vitro as also in vivo [2]. In this study, a new accurate liquid chromatographytandem mass spectrometry method was developed for simultaneous analysis of acrylamide and DMHF in baby food. To the best of our knowledge, this is the first study on DMHF in baby foods as well as pioneer in acrylamide monitoring in baby foods from Brazil. For this, a sample preparation method was optimized, which involves acetonitrile-based extraction, followed by dispersive solidphase extraction (dSPE) using primary and secondary amine sorbent (PSA) and then, a final cleanup of extract by solid phase extraction (SPE). An ACQUITY ultraperformance liquid chromatography (UPLC) system interfaced to a Quattro Premier XE triple quadrupole mass spectrometer with electrospray ionization (ESI) source (WATERS) was employed for analysis. The chromatographic separation was achieved on a C8 column (150 mm x 2.1 mm i.d., 5?m particle size) at 30ºC with a flow rate of 0.2 ml/min, and the injection volume was 20 ?l. The mobile phases consisted of MilliQ water (A) and acetonitrile (B), both containing 0.01% of formic acid, and the elution gradient employed was as follows: 0 min, 5% B?... Mostrar Tudo |
|
Palavras-Chave: |
Acrylamide metabolite; Baby Food; Contaminante; Food contaminant; Food contaminants; LCMS/MS. |
|
Thesagro: |
Poluente. |
|
Thesaurus NAL: |
Acrylamides; Infant foods. |
|
Categoria do assunto: |
W Química e Física |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/161184/1/2016RA-027.pdf
|
|
Marc: |
LEADER 04219nam a2200253 a 4500
001 2068083
005 2017-06-29
008 2016 bl uuuu u00u1 u #d
100 1 $aPETRARCA, M. H.
245 $aDevelopment, uncertainty estimation and application of a LC-MS/MS method for simultaneous determination of acrylamide and 4hydroxy2,5-dimethyl-3(2H)-furanone in baby food.$h[electronic resource]
260 $aIn: IBERO-AMERICAN, 1.; BrMASS CONFERENCE ON MASS SPECTROMETRY, 6., 2016, Rio de Janeiro. Resumos Eletrônicos... São Paulo: Sociedade Brasileira de Espectrometria de Massas$c2016
520 $aAcrylamide, a heatgenerated food contaminant, has been associated with neurotoxic effects in humans and was classified under group 2A as a probable human carcinogen by the International Agency Research on Cancer (IARC) [1]. Additionally, hydroxy2,5dimethyl3(2H)furanone (DMHF), a furanic compound, has demonstrated genotoxic effects in vitro as also in vivo [2]. In this study, a new accurate liquid chromatographytandem mass spectrometry method was developed for simultaneous analysis of acrylamide and DMHF in baby food. To the best of our knowledge, this is the first study on DMHF in baby foods as well as pioneer in acrylamide monitoring in baby foods from Brazil. For this, a sample preparation method was optimized, which involves acetonitrile-based extraction, followed by dispersive solidphase extraction (dSPE) using primary and secondary amine sorbent (PSA) and then, a final cleanup of extract by solid phase extraction (SPE). An ACQUITY ultraperformance liquid chromatography (UPLC) system interfaced to a Quattro Premier XE triple quadrupole mass spectrometer with electrospray ionization (ESI) source (WATERS) was employed for analysis. The chromatographic separation was achieved on a C8 column (150 mm x 2.1 mm i.d., 5?m particle size) at 30ºC with a flow rate of 0.2 ml/min, and the injection volume was 20 ?l. The mobile phases consisted of MilliQ water (A) and acetonitrile (B), both containing 0.01% of formic acid, and the elution gradient employed was as follows: 0 min, 5% B? 5 min, 50% B? 5.1 min, 100% B? 6?10 min 5% B, resulting in a total run time of 10 min. Data acquisition was carried out in selective reaction monitoring (SRM) mode employing ESI in positive mode, whose conditions were: capillary voltage 3 KV? temperatures of source at 120°C and desolvation at 400°C and gas flow 500 L/h. The collision energy and cone voltage were 10 and 25 V. The m/z 55 and 44 (acrylamide) and m/z 101 and 83 (DMHF) were used respectively for quantitative and confirmative purposes. As a great result, the optimized sample preparation method provided sufficient removal of potential analytical interfering commonly present in fruitbased matrices, the amino acid valine, without affecting the sensitivity, trueness and precision of the method. Limits of detection (LOD) and quantification (LOQ) of 10 and 20 ?g/kg were obtained for both analytes. Linearity in solventonly and matrixmatched calibration curves was achieved in the range between 10 and 300 ?g/kg, with determination coefficients higher than 0.99. Matrix effects, calculated from slopes obtained for solvent and matrixmatched calibrations, were observed for acrylamide (?45%) and DMHF(?11%). Recoveries between 75 and 115% were obtained for the two studied compounds from blank baby foods spiked at 20 (LOQ), 100 and 200 ?g/kg. Precision, under repeatability and withinlaboratory reproducibility conditions, was verified with RSD values < 20%. In addition, by using data obtained from inhouse validation, was possible to estimate the expanded measurement uncertainty for the levels of acrylamide and DMHF detected in the samples, whose values were lower than 25%. The applicability of proposed analytical method was evaluated by analyzing 20 baby food samples available in Brazilian markets.
650 $aAcrylamides
650 $aInfant foods
650 $aPoluente
653 $aAcrylamide metabolite
653 $aBaby Food
653 $aContaminante
653 $aFood contaminant
653 $aFood contaminants
653 $aLCMS/MS
700 1 $aROSA, M. A.
700 1 $aQUEIROZ, S. C. do N. de
700 1 $aGODOY, H. T.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Meio Ambiente (CNPMA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
