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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
16/11/2021 |
Data da última atualização: |
16/11/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
CHABI-JESUS, C.; RAMOS-GONZÁLEZ, P. L.; POSTCLAM-BARRO, M.; FONTENELE, RA. S.; HARAKAVA, R.; BASSANEZI, R. B.; MOREIRA, A. S.; KITAJIMA, E. W.; VARSANI, A.; ASTUA, J. de F. |
Afiliação: |
CAMILA CHABI-JESUS, ESALQ; PEDRO L. RAMOS-GONZÁLEZ, Instituto Biológico/IB; MATHEUS POSTCLAM-BARRO, Instituto Biológico/IB; RAFAELA SALGADO FONTENELE, Arizona State University; RICARDO HARAKAVA, Instituto Biológico/IB; RENATO B. BASSANEZI, Fundo de Defesa da Citricultura; ALECIO SOUZA MOREIRA, CNPMF; ELLIOT W. KITAJIMA, Instituto Biológico/IB; ARVIND VARSANI, Arizona State University; JULIANA DE FREITAS ASTUA, CNPMF. |
Título: |
Molecular epidemiology of Citrus Leprosis Virus C: a new viral lineage and phylodynamic of the main viral subpopulations in the Americas. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Frontiers in Microbiology, V. 12, 2021. |
Idioma: |
Inglês |
Conteúdo: |
Despite the importance of viral strains/variants as agents of emerging diseases, genetic and evolutionary processes affecting their ecology are not fully understood. To get insight into this topic, we assessed the population and spatial dynamic parameters of citrus leprosis virus C (CiLV-C, genus Cilevirus, family Kitaviridae). CiLV-C is the etiological agent of citrus leprosis disease, a non-systemic infection considered the main viral disorder affecting citrus orchards in Brazil. Overall, we obtained 18 complete or near-complete viral genomes, 123 complete nucleotide sequences of the open reading frame (ORF) encoding the putative coat protein, and 204 partial nucleotide sequences of the ORF encoding the movement protein, from 430 infected Citrus spp. samples collected between 1932 and 2020. A thorough examination of the collected dataset suggested that the CiLV-C population consists of the major lineages CRD and SJP, unevenly distributed, plus a third one called ASU identified in this work, which is represented by a single isolate found in an herbarium sample collected in Asuncion, Paraguay, in 1937. Viruses from the three lineages share about 85% nucleotide sequence identity and show signs of inter-clade recombination events. Members of the lineage CRD were identified both in commercial and non-commercial citrus orchards. However, those of the lineages SJP were exclusively detected in samples collected in the citrus belt of São Paulo and Minas Gerais, the leading Brazilian citrus production region, after 2015. The most recent common ancestor of viruses of the three lineages dates back to, at least, ?1500 years ago. Since citrus plants were introduced in the Americas by the Portuguese around the 1520s, the Bayesian phylodynamic analysis suggested that the ancestors of the main CiLV-C lineages likely originated in contact with native vegetation of South America. The intensive expansion of CRD and SJP lineages in Brazil started probably linked to the beginning of the local citrus industry. The high prevalence of CiLV-C in the citrus belt of Brazil likely ensues from the intensive connectivity between orchards, which represents a potential risk toward pathogen saturation across the region. MenosDespite the importance of viral strains/variants as agents of emerging diseases, genetic and evolutionary processes affecting their ecology are not fully understood. To get insight into this topic, we assessed the population and spatial dynamic parameters of citrus leprosis virus C (CiLV-C, genus Cilevirus, family Kitaviridae). CiLV-C is the etiological agent of citrus leprosis disease, a non-systemic infection considered the main viral disorder affecting citrus orchards in Brazil. Overall, we obtained 18 complete or near-complete viral genomes, 123 complete nucleotide sequences of the open reading frame (ORF) encoding the putative coat protein, and 204 partial nucleotide sequences of the ORF encoding the movement protein, from 430 infected Citrus spp. samples collected between 1932 and 2020. A thorough examination of the collected dataset suggested that the CiLV-C population consists of the major lineages CRD and SJP, unevenly distributed, plus a third one called ASU identified in this work, which is represented by a single isolate found in an herbarium sample collected in Asuncion, Paraguay, in 1937. Viruses from the three lineages share about 85% nucleotide sequence identity and show signs of inter-clade recombination events. Members of the lineage CRD were identified both in commercial and non-commercial citrus orchards. However, those of the lineages SJP were exclusively detected in samples collected in the citrus belt of São Paulo and Minas Gerais, the leading Brazilia... Mostrar Tudo |
Thesaurus Nal: |
Cilevirus; Citrus leprosis virus C; Plant diseases and disorders. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/227810/1/fmicb-12-641252.pdf
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Marc: |
LEADER 03057naa a2200265 a 4500 001 2136190 005 2021-11-16 008 2021 bl uuuu u00u1 u #d 100 1 $aCHABI-JESUS, C. 245 $aMolecular epidemiology of Citrus Leprosis Virus C$ba new viral lineage and phylodynamic of the main viral subpopulations in the Americas.$h[electronic resource] 260 $c2021 520 $aDespite the importance of viral strains/variants as agents of emerging diseases, genetic and evolutionary processes affecting their ecology are not fully understood. To get insight into this topic, we assessed the population and spatial dynamic parameters of citrus leprosis virus C (CiLV-C, genus Cilevirus, family Kitaviridae). CiLV-C is the etiological agent of citrus leprosis disease, a non-systemic infection considered the main viral disorder affecting citrus orchards in Brazil. Overall, we obtained 18 complete or near-complete viral genomes, 123 complete nucleotide sequences of the open reading frame (ORF) encoding the putative coat protein, and 204 partial nucleotide sequences of the ORF encoding the movement protein, from 430 infected Citrus spp. samples collected between 1932 and 2020. A thorough examination of the collected dataset suggested that the CiLV-C population consists of the major lineages CRD and SJP, unevenly distributed, plus a third one called ASU identified in this work, which is represented by a single isolate found in an herbarium sample collected in Asuncion, Paraguay, in 1937. Viruses from the three lineages share about 85% nucleotide sequence identity and show signs of inter-clade recombination events. Members of the lineage CRD were identified both in commercial and non-commercial citrus orchards. However, those of the lineages SJP were exclusively detected in samples collected in the citrus belt of São Paulo and Minas Gerais, the leading Brazilian citrus production region, after 2015. The most recent common ancestor of viruses of the three lineages dates back to, at least, ?1500 years ago. Since citrus plants were introduced in the Americas by the Portuguese around the 1520s, the Bayesian phylodynamic analysis suggested that the ancestors of the main CiLV-C lineages likely originated in contact with native vegetation of South America. The intensive expansion of CRD and SJP lineages in Brazil started probably linked to the beginning of the local citrus industry. The high prevalence of CiLV-C in the citrus belt of Brazil likely ensues from the intensive connectivity between orchards, which represents a potential risk toward pathogen saturation across the region. 650 $aCilevirus 650 $aCitrus leprosis virus C 650 $aPlant diseases and disorders 700 1 $aRAMOS-GONZÁLEZ, P. L. 700 1 $aPOSTCLAM-BARRO, M. 700 1 $aFONTENELE, RA. S. 700 1 $aHARAKAVA, R. 700 1 $aBASSANEZI, R. B. 700 1 $aMOREIRA, A. S. 700 1 $aKITAJIMA, E. W. 700 1 $aVARSANI, A. 700 1 $aASTUA, J. de F. 773 $tFrontiers in Microbiology, V. 12, 2021.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
28/04/2020 |
Data da última atualização: |
10/08/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
PINTO, P. H. N.; BALARO, M. F. A.; SARAIVA, H. F. R. de A.; BRAIR, V. L.; ALFRADIQUE, V. A. P.; CÔRTESA, L. R.; COSENTINO, I. O.; SOUZA-FABJANA, J. M. G.; FONSECA, J. F. da; BRANDÃO, F. Z. |
Afiliação: |
PEDRO HENRIQUE NICOLAU PINTO, Universidade Federal Fluminense; MARIO FELIPE ALVAREZ BALARO, Universidade Federal Fluminense; HELENA FABIANA REIS DE ALMEIDA SARAIVA, Universidade Federal Fluminense; VIVIANE LOPES BRAIR, Universidade Federal Fluminense; VIVIAN ANGÉLICO PEREIRA ALFRADIQUE, Universidade Federal Fluminense; LUANA RANGEL CÔRTESA, Universidade Federal Fluminense; ISABEL OLIVEIRA COSENTINO, Universidade Federal Fluminense; JOANNA MARIA GONCALVES SOUZA-FABJANA; JEFERSON FERREIRA DA FONSECA, CNPC; FELIPE ZANDONADI BRANDÃO, Universidade Federal Fluminense. |
Título: |
Successive in vivo embryo production in Santa Inês sheep. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Animal Production Science, v. 60, n. 4, p. 497-502, Dec. 2020. |
DOI: |
https://doi.org/10.1071/AN18740 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: Context. In vivoembryo production, also called multiple ovulation and embryo transfer, can accelerate genetic gain, andthus improve animal production. However, there are issues limiting a wider use of this biotechnology in sheep livestock.Aims.This study aimed to determine (1) whether a previous response to superovulation (SOV) can be used as a criterionto select ewes forin vivoembryo production, (2) whether the intensity of the SOV response (number of corpora lutea, CL)can affect the embryo recovery rate, and (3) whether the number of CL quantified by colour Doppler ultrasonography can beused to calculate the recovery rate.Methods.Twenty-five Santa Inês ewes underwent SOV three times (SOV1, SOV2 and SOV3), with 200 mg FSH andnatural mating. The number of CL after each SOV was determined by laparoscopy and by colour Doppler ultrasonography.Key results.The number of CL significantly decreased (P<0.05) after SOV1 (7.54.8) to 3.05.0 at SOV 2 and2.23.5 at SOV3. Strong correlations were observed between SOV2 and SOV3 in terms of numbers of CL (r= 0.86,r2= 0.74;P<0.0001) and viable embryos (r= 0.79,r2= 0.63;P<00001). However, no correlations were observed betweenSOV1 and SOV2 or between SOV1 and SOV3. Recovery rate did not differ with the intensity of the SOV response(6, 7?10,>10 CL) or between the methods used to quantify CL.Conclusions.Ewes did not show the same pattern of response when submitted to successive FSH-based SOV. Theintensity of the SOV response did not affect the recovery rate, and the number of CL estimated by colour Dopplerultrasonography can be used to calculate the recovery rate.Implications.Selecting sheep embryo donors by a previous SOV response is not always feasible. The recovery rate ishomogeneous and it is not affected by the intensity of the SOV response. A nonsurgical technique can be used to assess therecovery rate, improving animal welfare in MOET programs. MenosAbstract: Context. In vivoembryo production, also called multiple ovulation and embryo transfer, can accelerate genetic gain, andthus improve animal production. However, there are issues limiting a wider use of this biotechnology in sheep livestock.Aims.This study aimed to determine (1) whether a previous response to superovulation (SOV) can be used as a criterionto select ewes forin vivoembryo production, (2) whether the intensity of the SOV response (number of corpora lutea, CL)can affect the embryo recovery rate, and (3) whether the number of CL quantified by colour Doppler ultrasonography can beused to calculate the recovery rate.Methods.Twenty-five Santa Inês ewes underwent SOV three times (SOV1, SOV2 and SOV3), with 200 mg FSH andnatural mating. The number of CL after each SOV was determined by laparoscopy and by colour Doppler ultrasonography.Key results.The number of CL significantly decreased (P<0.05) after SOV1 (7.54.8) to 3.05.0 at SOV 2 and2.23.5 at SOV3. Strong correlations were observed between SOV2 and SOV3 in terms of numbers of CL (r= 0.86,r2= 0.74;P<0.0001) and viable embryos (r= 0.79,r2= 0.63;P<00001). However, no correlations were observed betweenSOV1 and SOV2 or between SOV1 and SOV3. Recovery rate did not differ with the intensity of the SOV response(6, 7?10,>10 CL) or between the methods used to quantify CL.Conclusions.Ewes did not show the same pattern of response when submitted to successive FSH-based SOV. Theintensity of the SOV response did not aff... Mostrar Tudo |
Palavras-Chave: |
MOET; Ovarian superstimulation. |
Thesagro: |
Corpo Lúteo; Melhoramento Genético Animal; Ovino; Reprodução Animal; Superovulação; Transferência de Embrião. |
Thesaurus NAL: |
Animal reproduction; Breeding and Genetic Improvement; Corpus luteum; Embryo transfer; Ewes; Superovulation. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 03132naa a2200409 a 4500 001 2121864 005 2021-08-10 008 2020 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1071/AN18740$2DOI 100 1 $aPINTO, P. H. N. 245 $aSuccessive in vivo embryo production in Santa Inês sheep.$h[electronic resource] 260 $c2020 520 $aAbstract: Context. In vivoembryo production, also called multiple ovulation and embryo transfer, can accelerate genetic gain, andthus improve animal production. However, there are issues limiting a wider use of this biotechnology in sheep livestock.Aims.This study aimed to determine (1) whether a previous response to superovulation (SOV) can be used as a criterionto select ewes forin vivoembryo production, (2) whether the intensity of the SOV response (number of corpora lutea, CL)can affect the embryo recovery rate, and (3) whether the number of CL quantified by colour Doppler ultrasonography can beused to calculate the recovery rate.Methods.Twenty-five Santa Inês ewes underwent SOV three times (SOV1, SOV2 and SOV3), with 200 mg FSH andnatural mating. The number of CL after each SOV was determined by laparoscopy and by colour Doppler ultrasonography.Key results.The number of CL significantly decreased (P<0.05) after SOV1 (7.54.8) to 3.05.0 at SOV 2 and2.23.5 at SOV3. Strong correlations were observed between SOV2 and SOV3 in terms of numbers of CL (r= 0.86,r2= 0.74;P<0.0001) and viable embryos (r= 0.79,r2= 0.63;P<00001). However, no correlations were observed betweenSOV1 and SOV2 or between SOV1 and SOV3. Recovery rate did not differ with the intensity of the SOV response(6, 7?10,>10 CL) or between the methods used to quantify CL.Conclusions.Ewes did not show the same pattern of response when submitted to successive FSH-based SOV. Theintensity of the SOV response did not affect the recovery rate, and the number of CL estimated by colour Dopplerultrasonography can be used to calculate the recovery rate.Implications.Selecting sheep embryo donors by a previous SOV response is not always feasible. The recovery rate ishomogeneous and it is not affected by the intensity of the SOV response. A nonsurgical technique can be used to assess therecovery rate, improving animal welfare in MOET programs. 650 $aAnimal reproduction 650 $aBreeding and Genetic Improvement 650 $aCorpus luteum 650 $aEmbryo transfer 650 $aEwes 650 $aSuperovulation 650 $aCorpo Lúteo 650 $aMelhoramento Genético Animal 650 $aOvino 650 $aReprodução Animal 650 $aSuperovulação 650 $aTransferência de Embrião 653 $aMOET 653 $aOvarian superstimulation 700 1 $aBALARO, M. F. A. 700 1 $aSARAIVA, H. F. R. de A. 700 1 $aBRAIR, V. L. 700 1 $aALFRADIQUE, V. A. P. 700 1 $aCÔRTESA, L. R. 700 1 $aCOSENTINO, I. O. 700 1 $aSOUZA-FABJANA, J. M. G. 700 1 $aFONSECA, J. F. da 700 1 $aBRANDÃO, F. Z. 773 $tAnimal Production Science$gv. 60, n. 4, p. 497-502, Dec. 2020.
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