03132naa a2200409 a 450000100080000000500110000800800410001902400410006010000200010124500860012126000090020752019360021665000240215265000370217665000180221365000200223165000090225165000190226065000170227965000340229665000100233065000240234065000200236465000310238465300090241565300290242470000210245370000280247470000170250270000250251970000200254470000210256470000280258570000220261370000200263577300670265521218642021-08-10       2020    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1071/AN187402DOI1 aPINTO, P. H. N.  aSuccessive in vivo embryo production in Santa Inês sheep.h[electronic resource]  c2020  aAbstract: Context. In vivoembryo production, also called multiple ovulation and embryo transfer, can accelerate genetic gain, andthus improve animal production. However, there are issues limiting a wider use of this biotechnology in sheep livestock.Aims.This study aimed to determine (1) whether a previous response to superovulation (SOV) can be used as a criterionto select ewes forin vivoembryo production, (2) whether the intensity of the SOV response (number of corpora lutea, CL)can affect the embryo recovery rate, and (3) whether the number of CL quantified by colour Doppler ultrasonography can beused to calculate the recovery rate.Methods.Twenty-five Santa Inês ewes underwent SOV three times (SOV1, SOV2 and SOV3), with 200 mg FSH andnatural mating. The number of CL after each SOV was determined by laparoscopy and by colour Doppler ultrasonography.Key results.The number of CL significantly decreased (P&lt0.05) after SOV1 (7.54.8) to 3.05.0 at SOV 2 and2.23.5 at SOV3. Strong correlations were observed between SOV2 and SOV3 in terms of numbers of CL (r= 0.86,r2= 0.74;P&lt0.0001) and viable embryos (r= 0.79,r2= 0.63;P&lt00001). However, no correlations were observed betweenSOV1 and SOV2 or between SOV1 and SOV3. Recovery rate did not differ with the intensity of the SOV response(6, 7?10,&gt10 CL) or between the methods used to quantify CL.Conclusions.Ewes did not show the same pattern of response when submitted to successive FSH-based SOV. Theintensity of the SOV response did not affect the recovery rate, and the number of CL estimated by colour Dopplerultrasonography can be used to calculate the recovery rate.Implications.Selecting sheep embryo donors by a previous SOV response is not always feasible. The recovery rate ishomogeneous and it is not affected by the intensity of the SOV response. A nonsurgical technique can be used to assess therecovery rate, improving animal welfare in MOET programs.  aAnimal reproduction  aBreeding and Genetic Improvement  aCorpus luteum  aEmbryo transfer  aEwes  aSuperovulation  aCorpo Lúteo  aMelhoramento Genético Animal  aOvino  aReprodução Animal  aSuperovulação  aTransferência de Embrião  aMOET  aOvarian superstimulation1 aBALARO, M. F. A.1 aSARAIVA, H. F. R. de A.1 aBRAIR, V. L.1 aALFRADIQUE, V. A. P.1 aCÔRTESA, L. R.1 aCOSENTINO, I. O.1 aSOUZA-FABJANA, J. M. G.1 aFONSECA, J. F. da1 aBRANDÃO, F. Z.  tAnimal Production Sciencegv. 60, n. 4, p. 497-502, Dec. 2020.