|
|
Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
14/01/2020 |
Data da última atualização: |
06/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
FONSECA, J. F. da; BATISTA, R. I. T. P.; TREVIZAN, J. T.; SOUZA-FABJAN, J. M. G.; BRANDÃO, F. Z.; CAMARGO, L. S. de A. |
Afiliação: |
JEFERSON FERREIRA DA FONSECA, CNPC; Ribrio Ivan Tavares Pereira Batista; Juliane Teramachi Trevizan; Joanna Maria Gonçalves Souza-Fabjan; Felipe Zandonadi Brandão; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
Título: |
Goat incubator: can bovine oocytes be matured in the uterine horn of a goat? |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Semina: Ciências Agrárias, Londrina, v. 40, n. 6, supp. 3, p. 3789-3796, 2019. |
DOI: |
10.5433/1679-0359.2019v40n6Supl3p3789 |
Idioma: |
Inglês |
Conteúdo: |
Abstract We used a goat as a live incubator, along with associated nonsurgical embryo transfer techniques, to perform ex situ (in vivo) maturation of bovine oocytes. Immature bovine cumulus-oocyte complexes (COCs) aspirated from 3-8 mm follicles from slaughterhouse ovaries were randomly split into two groups for in vitro (IVM; n = 38) and ex situ maturation (ESM; n = 40). IVM was performed for a period of 24 h at 38.5 ºC and with 5% CO2 in the air of maximum humidity. For ESM, a presynchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apiece, via the transcervical route. After 24 h the structures were retrieved through uterine flushing. Analyses of nuclear maturation and lipid quantification were performed on oocytes from both groups. Fluorescent intensity was compared using the Student?s t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for the ESM and IVM groups, respectively. In vitro-matured COCs contained more lipid droplets, expressed as a higher amount (p < 0.05) of emitted fluorescent light than ex situ-matured COCs (858 ± 73 vs. 550 ± 64 arbitrary fluorescence units, respectively). This is the first report to associate nonsurgical embryo transfer techniques and a goat as a live incubator for the maturation of bovine oocytes. We conclude that bovine oocytes can progress meiotically in the uterus horn of a goat and that transcervical transfer of bovine oocytes to a goat?s uterus could present an alternative to nuclear maturation. MenosAbstract We used a goat as a live incubator, along with associated nonsurgical embryo transfer techniques, to perform ex situ (in vivo) maturation of bovine oocytes. Immature bovine cumulus-oocyte complexes (COCs) aspirated from 3-8 mm follicles from slaughterhouse ovaries were randomly split into two groups for in vitro (IVM; n = 38) and ex situ maturation (ESM; n = 40). IVM was performed for a period of 24 h at 38.5 ºC and with 5% CO2 in the air of maximum humidity. For ESM, a presynchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apiece, via the transcervical route. After 24 h the structures were retrieved through uterine flushing. Analyses of nuclear maturation and lipid quantification were performed on oocytes from both groups. Fluorescent intensity was compared using the Student?s t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for the ESM and IVM groups, respectively. In vitro-matured COCs contained more lipid droplets, expressed as a higher amount (p < 0.05) of emitted fluorescent light than ex situ-matured COCs (858 ± 73 vs. 550 ± 64 arbitrary fluorescence units, respectively). This is the first report to associate nonsurgical embryo transfer techniques and a goat as a live incubator for the maturation of bovine oocytes. We conclude that bovine oocytes can progress meiotically in the uterus horn of a goat and that trans... Mostrar Tudo |
Palavras-Chave: |
Bovine oocytes; COCs; Ex-situ maturation; In vitro maturation; Nonsurgical embryo transfer technique. |
Thesaurus Nal: |
Goats. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/208736/1/Goat-incubator-can-bovine-oocytes.pdf
|
Marc: |
LEADER 02452naa a2200265 a 4500 001 2118709 005 2024-02-06 008 2019 bl uuuu u00u1 u #d 024 7 $a10.5433/1679-0359.2019v40n6Supl3p3789$2DOI 100 1 $aFONSECA, J. F. da 245 $aGoat incubator$bcan bovine oocytes be matured in the uterine horn of a goat?$h[electronic resource] 260 $c2019 520 $aAbstract We used a goat as a live incubator, along with associated nonsurgical embryo transfer techniques, to perform ex situ (in vivo) maturation of bovine oocytes. Immature bovine cumulus-oocyte complexes (COCs) aspirated from 3-8 mm follicles from slaughterhouse ovaries were randomly split into two groups for in vitro (IVM; n = 38) and ex situ maturation (ESM; n = 40). IVM was performed for a period of 24 h at 38.5 ºC and with 5% CO2 in the air of maximum humidity. For ESM, a presynchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apiece, via the transcervical route. After 24 h the structures were retrieved through uterine flushing. Analyses of nuclear maturation and lipid quantification were performed on oocytes from both groups. Fluorescent intensity was compared using the Student?s t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for the ESM and IVM groups, respectively. In vitro-matured COCs contained more lipid droplets, expressed as a higher amount (p < 0.05) of emitted fluorescent light than ex situ-matured COCs (858 ± 73 vs. 550 ± 64 arbitrary fluorescence units, respectively). This is the first report to associate nonsurgical embryo transfer techniques and a goat as a live incubator for the maturation of bovine oocytes. We conclude that bovine oocytes can progress meiotically in the uterus horn of a goat and that transcervical transfer of bovine oocytes to a goat?s uterus could present an alternative to nuclear maturation. 650 $aGoats 653 $aBovine oocytes 653 $aCOCs 653 $aEx-situ maturation 653 $aIn vitro maturation 653 $aNonsurgical embryo transfer technique 700 1 $aBATISTA, R. I. T. P. 700 1 $aTREVIZAN, J. T. 700 1 $aSOUZA-FABJAN, J. M. G. 700 1 $aBRANDÃO, F. Z. 700 1 $aCAMARGO, L. S. de A. 773 $tSemina: Ciências Agrárias, Londrina$gv. 40, n. 6, supp. 3, p. 3789-3796, 2019.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
01/09/2003 |
Data da última atualização: |
10/04/2008 |
Autoria: |
CARNEIRO, R. M. D. G.; MARTINS, I.; NEVES, D. I.; SILVA, S. A.; CORDEIRO, C. M. T. |
Título: |
Preservação eficiente dos nematóides de galhas em nitrogênio líquido. |
Ano de publicação: |
2003 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE NEMATOLOGIA=MEETING OF THE BRAZILIAN SOCIETY OF NEMATOLOGY, 24., 2003, Petrolina. [Anais...]. Petrolina: Sociedade Brasileira de Nematologia: Embrapa Semi-Árido, 2003. |
Páginas: |
p. 119. |
Idioma: |
Português |
Palavras-Chave: |
Nematóides de galhas; Nitrogênio líquido. |
Thesaurus NAL: |
Meloidogyne. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00711nam a2200193 a 4500 001 1184760 005 2008-04-10 008 2003 bl uuuu u01u1 u #d 100 1 $aCARNEIRO, R. M. D. G. 245 $aPreservação eficiente dos nematóides de galhas em nitrogênio líquido. 260 $aIn: CONGRESSO BRASILEIRO DE NEMATOLOGIA=MEETING OF THE BRAZILIAN SOCIETY OF NEMATOLOGY, 24., 2003, Petrolina. [Anais...]. Petrolina: Sociedade Brasileira de Nematologia: Embrapa Semi-Árido$c2003 300 $ap. 119. 650 $aMeloidogyne 653 $aNematóides de galhas 653 $aNitrogênio líquido 700 1 $aMARTINS, I. 700 1 $aNEVES, D. I. 700 1 $aSILVA, S. A. 700 1 $aCORDEIRO, C. M. T.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|